Abstract
Effects of calcium channel blocker verapamil on spinal reflexes were studied. Verapamil 5, 50, (iM locally. 10,20 mg/kg was administradet intraperitoneally and 10, 50, 100 (iM localyy.
Experiments on adult spinal cats (n=10) were conducted. Animals weighing 1,5-3 kg were anesthetized with ketamine (45 mg/kg intramuscular) and artiflcally ventilated. Animals were spinalized at Cj level and a laminectomy was performed in the lumbosacral region. The ventral and dorsal roots of segment Lg were isolated. A polyethylene cannule was int¬roduced into the carotid artery to monitor blood pressure, which was kept above 100 mmHg. The dorsal root of segment L5 placed on a silver-silver chloride wire electrod for electrical stimulation. The reflex potentials were recorded from the ipsilateral L5 ventral root, mounted on a silver-silver chloride wire electrode. Both systemic and local applica¬tion of verapamil inhibited reflexes (p<0.05). The latency of reflexes was considerably inc¬reased as a results of systemic 20 mg doses (p<0.05).
These results show the possible presence of voltage-dependent calcium channels in the spinal cord and these channels may play an important role to regulate reflex responds.
Kalsiyum kanal blokerlerinden verapamilin omurilik reflekslerine olan etkilerini araştırdık. Verapamil, 10, 50, 100 |iM lokal ve 5, 10, 20 mg/kg intraperitonal (i.p.) olarak verildi. Yetişkin kediler (n=10) 1,5 - 3 kg ağırlığında intramüsküler (i.m.) ketaminle (50 mg/kg, i.m.) anestezi edildi ve suni ventilasyon uygulandı. Hayvanlar servikal seviyeden spinalize edildi. Lumbosakral bölgeden laminektomi yapıldı. L5 segmentin dorsal ve ventral kökleri izole edildi.
Stimülasyon için L5 segmentin dorsal köküne Ag-AgCI tel elektrot yerleştirildi. Refleks po-tansiyeller ipsilateral L5 ventral kökten kaydedildi, bu kayıtta da Ag-AgCI tel elektrot kul¬lanıldı.
Verapamilin sistemikve lokal dozları refleks cevap amplitüdünü azalttı (p<0.05). 20 mg/ kg'lık sistemik doz latenste anlamlı uzama oluşturdu (p<0.05).
Bu sonuçlar bize omurilikte voltaja bağımlı kalsiyum kanallarının olabileceğini ve refleks cevabın düzenlenmesinde voltaj kapılı kalsiyum kanallarının rol oynayabileceğini düşündürmektedir.
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| Primary Language | English |
|---|---|
| Journal Section | Basic Medical Sciences |
| Authors | |
| Publication Date | December 23, 2009 |
| Submission Date | December 13, 2009 |
| Published in Issue | Year 1996 Volume: 13 Issue: 2 |
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