Astrositik Neoplazilerde Fascin Ekspresyonu‡

Year 2008, Volume: 25 Issue: 2, 45 - 49, 29.12.2009

Levent Yıldız Mehmet Kefeli Oğuz Aydın Bedri Kandemir

Abstract

Fascin 55 kD ağırlığında aktin bağlayan bir proteindir. Hücre membranlarında bulunur ve hücresel motiliteyi başlatır. Fascin bazı malign tümörlerin biyolojik davranışı ile bağlantılıdır. Biz astrositom ve gliozis olgularında immünohistokimyasal olarak fascin ekspresyonunu araştırdık. İmmünohistokimyasal çalışma 28 astrositik tümör ve 6 gliozis olgusunda gerçekleştirildi. Glial hücreler ve tümör hücrelerinde fascin pozitifliği şu şekilde derecelendirildi; boyanma: %0-4 (-), %5-25 (1+), %26-50 (2+), %51-75 (3+) ve %75’in üzeri 4(+).Yirmi sekiz hastanın 17’si fascin pozitif boyandı (%60.7). Yedi pilositik astrositom (WHO grade I) olgusunun 2’si (%28.5), 6 diffüz astrositom (WHO grade II) olgusunun 4’ü (%66.6), 8 anaplastik astrositom (WHO grade III) olgusunun 4’ü (%50) fascin pozitif boyandı. Glioblastom olgularının ise tamamı (7 olgu) (%100) fascin ile pozitif boyandı. Gliozis olgularının tamamında da (6 olgu) (%100) fascin boyanması gözlendi. Tümör grubunda 14 tümör olgusu 4 (+), 3 tümör olgusu 3 (+) düzeyinde boyanma gösteriyordu. Karşılaştırıldığında 13 pilositik ve diffüz astrositom (WHO grade I ve II) olgusunun 6’sı (%46.1) fascin ile pozitif boyanma gösterirken, 15 anaplastik astrositom ve glioblastom (WHO grade III-I ve IV) olgusunun 11’inin (%73.3) fascin ile pozitif boyandığı gözlendi. Daha yüksek dereceli astrositomlar genel olarak daha yüksek derecelerde fascin boyanması gösterdiler. Astrositik tümörlerin malign biyolojik davranışında fascin ekspresyonu rol oynuyor olabilir.



Fascin Expression in Astrocytic Neoplasms
Fascin is a 55-kd actin binding protein. It is found in cell membranes and promoting cellular motility. Fascin has been linked to the malignant biological behavior of some tumors. We investigated immunuhistochemically fascin expression in astrocytomas and gliosis cases. Immunohistochemical study was performed in 28 astrocytic tumors and 6 gliosis. Fascin positivity of tumor cells and glial cells was graded as follows: Staining: 0% to 4%, (-), 5% to 25%, (1+), 26% to 50%, (2+), 51% to 75%, (3+), more than 75%, (4+). Seventeen of 28 cases showed positive fascin staining (60.7%). Two of 7 (28.5%) pilocytic astrocytoma (WHO grade I) cases, 4 of 6 (66.6%) diffuse astrocytoma (WHO grade II) cases and 4 of 8 (50%) anaplastic astrocytoma (WHO grade III) cases showed positive fascin staining. All of glioblastoma cases (7 cases) (100%) was positive. All of gliosis cases (6 cases) (100%) was positive. In tumor group, 14 tumors showed 4 (+) positive staining and 3 tumors had 3 (+) staining. In comparison, 6 of 13 pilocytic and diffuse astrocytoma (WHO grade I and II) cases had fascin positivity (46.1%), 11 of 15 anaplastic astrocytoma and glioblastoma (WHO grade III and IV) cases had fascin positivity (73.3%). Higher grade astrocytomas generally expressed a greater degree of fascin staining. Fascin expression may play a role in malignant biological behavior of astrocytic neoplasms.

Keywords

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References

• Hashimoto Y, Skacel M, Adams JC. Roles of fascin in human carcinoma motility and signaling: Prospect for a novel biomarker? Int J Biochem Cell Biol 2005; 37: 1787–1804.
• Adams JC. Roles of fascin in cell adhesion and motility. Curr Opin Cell Biol 2004; 16: 590–596.
• Goncharuk VN, Ross JS, Carlson JA. Actin-binding protein fascin expression in skin neoplasia. J Cutan Pathol 2002; 29: 430–438.
• Ross R, Jonuleit H, Bros M, et al. Expression of the actin-bundling protein fascin in cultured human dentritic cells correlates with dentritic morphology and cell differentiation. J Invest Dermatol 2000; 115: 658–663.
• Pelosi G, Pastorino U, Pasini F, et al. Independent prognostic value of fascin immunoreactivitiy in stage I non-small cell lung cancer. Br J Cancer 2003; 88: 537–547.
• Hashimoto Y, Ito T, Inoue H, et al. Prognostic signifi- cance of fascin overexpression in human esophageal squamous cell carcinoma. Clin Cancer Res 2005, 11: 2597–2605.
• Hashimoto Y, Shimada Y, Kawamura J, et al. The prognostic relevance of fascin expression in human gastric carcinoma. Oncology 2004; 67: 262–270.
• Yoder BJ, Tso E, Skacel M, et al. The expression of fascin, an actin-bundling motility protein, correlates with hormone receptor-negative cancer and a more aggressive clinical course. Clin Cancer Res 2005; 11: 186–192.
• Peraud A, Mondal S, Hawkins C, et al. Expression of fascin, an actin-bundling protein, in astrocytoma varying grades. Brain Tumor Pathol 2003; 20: 53–58.
• Roma AA, Prayson RA. Fascin expression in 90
• patients with glioblastoma multiforme. Ann Diagn
• Pathol 2005; 9: 307–311.