The effect of the antioxidant drug “U-74389G” on alkaline phosphatase levels during ischemia reperfusion injury in rats

Year 2014, Volume: 31 Issue: 2, 0 - , 10.09.2014

Constantinos Tsompos , Constantinos Panoulis Konstantinos Toutouzas George Zografos Apostolos Papalois

Abstract

The aim of this experimental study was to examine the effect of the antioxidant drug “U-74389G”, on rat model and particularly in a liver ischemia reperfusion (IR) protocol. The beneficial effect or non effectiveness of that molecule were studied biochemically using mean blood alkaline phosphatase levels (ALP). Forty rats of mean weight 231.875 gr were used in the study. ALP levels were measured 60 min after reperfusion (groups A and C) and 120 min after reperfusion (groups B and D) with administration of the drug U-74389G in groups C and D. U-74389G administration non significantly increased the ALP levels by 19.76%±15.06% (p=0.1784), but by 31.91%±7.69% (p=0.0001) for predicted values. Reperfusion time non-significantly decreased the ALP levels by 25.21%±14.83% (p=0.0675), also by 6.38%±9.30% (p=0.3650) for predicted values. However, U-74389G administration and reperfusion time together non-significantly increased the ALP levels by 8.08%±9.19% (p=0.3696), but also by 17.75%±4.79% (p=0.0005) for predicted values. This study indicates that U-74389G administration and its interaction with reperfusion time non significantly increased short-term effects on ALP levels. However, the predicted ALP values adjusted for rats weight, kept significantly increased ones, which stand for the restorating effects of U-74389G, that are slower than it seems.

Keywords

Alkaline phosphatase levels; ischemia; reperfusion; U-74389G

References

• Ansermet, F., Mieville, C., Diezi, J., 1980. Urinary enzyme excretion and changes in renal functions induced by toxic substances or by renal ischemia in rats. Arch. Toxicol. 4, 201-207.
• Burlacu, A., Rosca, A.M., Maniu, H., Titorencu, I., Dragan, E., Jinga, V., Simionescu, M., 2008. Promoting effect of 5-azacytidine on the myoge- nic differentiation of bone marrow stromal cells. Eur. J. Cell. Biol. 87, 173-184.
• Coux, G., Trumper, L., Elías, M.M., 2002. Renal function and cortical (Na(+)+K(+))-ATPase activity, abundance and distribution after ischae- mia-reperfusion in rats. Biochim. Biophys. Acta. 2, 71-80.
• Doty, S.B., Morey-Holton, E.R., Durnova, G.N., Kaplansky, A.S., 1990. Cosmos 1887: Morphology, histochemistry, and vasculature of the growing rat tibia. FASEB J. 4, 16-23.
• Fenglin, S., Jennifer C., Kenneth L.A., 1995. 21-aminosteroid and 2-(aminomethyl) chromans inhibition of arachidonic acid-induced lipid peroxidation and permeability enhancement in bovine brain microvessel endothelial cell monolayers. Free Radic. Biol. Med. 19, 349-357.
• Hahn, O., Szijártó, A., Lotz, G., Schaff, Z., Vígváry, Z., Váli, L., Kupcsulik, P.K., 2007. The effect of ischemic preconditioning prior to intrao- perative radiotherapy on ischemic and on reperfused rat liver. J. Surg. Res. 142, 32-44.
• Koyama, T., Xie, Z., Gao, M., Suzuki, J., Batra, S., 1998. Adaptive changes in the capillary network in the left ventricle of rat heart. Jpn J. Phy- siol. 48, 229-241.
• Seifert, J., Frelich, A., Shecterle, L., St Cyr, J., 2008. Assessment of hematological and biochemical parameters with extended D-Ribose inges- tion. J. Int. Soc. Sports Nutr. 15, 5, 13. doi: 10.1186/1550-2783-5-13.