The Importance of Aminoguanidine and Methylprednisolone Administration in Lung Contusion after Chest Trauma

Year 2021, Volume: 38 Issue: 4, 504 - 510, 09.10.2021

Fatih Çalışkan , Hızır Ufuk Akdemir , Celal Katı , Latif Duran , Tolga Güvenç

Abstract

ABSTRACT
Background: This study aims to evaluate the effect of the antioxidant and antiinflammatory properties of aminoguanidine and metylprednisolone (MP) on lung tissue in a pulmonary contusion model of rats and evaluate whether their combined use improves treatment efficacy.
Methods: This study included 35 female Sprague Dawley rats weighing 250-300 grams. The rats were divided into five groups as following: Sham; Pulmonary Contusion (PC); PC+MP, PC group treated with i.p methylprednisolone; PC+AG, PC group treated with i.p Aminoguanidine; and PC+AG+MP, PC group treated with Aminoguanidine and methylprednisolone. Each group had seven animals. Blood and lung tissues were studied biochemically and histopathologically.
Results: When compared groups according to serum levels of biomarkers, serum YKL-40, nitrate-nitrite, catalase, and TBARS levels were significant different. Serum YKL-40 levels were decreased after treatments in three groups. The serum YKL-40 levels in PC+AG group were lower than the other treatment groups, especially compared to PC + MP (p=0,028). Serum nitrate-nitrite levels were decreased in all treatment groups (PC+MP, PC+AG and PC+MP+AG). The lowest levels were measured in PC+MP+AG; but there was no statistically significant difference compared to PC group (p>0.05). Serum catalase levels were increased in all treatment groups. The higher levels were measured in PC+MP+AG than the other single treatment groups; however PC+MP+AG and PC+MP were statistically significant different compared to PC group (p=0,001 and p=0,002 respectively). Serum TBARS levels were decreased in all treatment groups compared to Sham group (p<0.001) and PC group (p<0.001). The lowest levels were measured in PC+MP+AG compared to PC group (p<0.001). Histopatologic and immunohistochemical staining scores were decreased at all the treatment groups, especially PC+MP+AG.
Conclusions: We suggest the use of combined treatment of methylprednisolone and aminoguanidine for the treatment of pulmonary contusion.

Keywords

oxidative stress, lung contusion, aminoguanidine, methylprednisolone

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