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Association of CYP24A1 gene, Vitamin D deficiency and heart diseases in Pakistani patients

Yıl 2022, Cilt: 39 Sayı: 2, 497 - 502, 18.03.2022

Öz

Background &Aims
To analyze the association of CYP24A1 gene polymorphism with vitamin D deficiency and its related cardiovascular disorders in the Pakistani population.
Materials and Methods
The present prospective cohort study was carried out at KRL and Rawalpindi General Hospital from January 2017 to December 2018. A total of 110 subjects suffering from heart diseases like hypertension, myocardial infarction, or congestive cardiac failure (age range 12-90 years) were enrolled in this study with informed consent. Sampling was done by non-probability convenience sampling. Blood 24Hydroxyvitmin D levels were assessed in the participants by using an electrochemiluminescence system. Genetic polymorphism in the CYP24A1 gene was screened in selected patients (n=15) through PCR-RFLP, after genomic DNA extraction from the whole blood.
Data were analyzed using SPSS version 24 for Mac. Fisher’s exact test and chi-square tests were applied for qualitative variables. The risk of polymorphism in CYP24A1(SNP rs6013897) genotypes TT, AT, and AA was determined by calculating the odds ratio and confidence interval. P-value <0.05 was considered statistically significant.
Results
We observed the highest level of vitamin D deficiency in patients of age group 18-45 years and insufficiency in age group 50-60years. A greater percentage of female patients (34.19%) were deficient in Vitamin D as compared to males (25.72%). Vitamin D deficiency was found to be associated with cardiovascular diseases like hypertension (P=0.000*), myocardial infarction(P=0.334), and heart failure (0.001*). CYP24A1 (SNP rs6013897) was significantly associated (P=0.000*) with vitamin D deficiency and heart diseases (P=0.004*). Moreover, significant polymorphism of genotype AT was observed in our subjects. {P=0.007*, Cramer’s V =.003 and 95% CI (0.44-17.27)}
Conclusion
There is an association of CYP24A1 gene polymorphism with vitamin D deficiency and heart diseases. Prevention of low vitamin D levels in individuals with this known genetic marker may help to avert the development of heart diseases in Pakistan.

Destekleyen Kurum

KRL & RGH

Proje Numarası

12/2016

Kaynakça

  • 1. Jorge A, Cordeiro J, Rosa M, Bianchi D. Vitamin D Deficiency and Cardiovascular Diseases. International Journal of Cardiovascular Sciences. 2018;31(4):2
  • 2. Latic N, Erben RG. Vitamin D and cardiovascular disease, with emphasis on hypertension, atherosclerosis, and heart failure. International Journal of Molecular Sciences. 2020 Jan;21(18):6483.
  • 3. Riaz H, Finlayson A, Bashir S, Hussain S, Mahmood S, Malik F et al. Prevalence of Vitamin D deficiency in Pakistan and implications for the future. Expert Review of Clinical Pharmacology. 2016;9(2):329-338.
  • 4. Krist AH, Davidson KW, Mangione CM, Cabana M, Caughey AB, Davis EM, Donahue KE, Doubeni CA, Epling JW, Kubik M, Li L. Screening for Vitamin D Deficiency in Adults: US Preventive Services Task Force Recommendation Statement. JAMA. 2021 Apr 13;325(14):1436-42.
  • 5. de la Guía-Galipienso F, Martínez-Ferran M, Vallecillo N, Lavie CJ, Sanchis-Gomar F, Pareja-Galeano H. Vitamin D and cardiovascular health. Clinical Nutrition. 2020 Dec 29.
  • 6. Klomp SD, Manson ML, Guchelaar HJ, Swen JJ. Phenoconversion of cytochrome P450 metabolism: a systematic review. Journal of Clinical Medicine. 2020 Sep;9(9):2890.
  • 7. Jayaraj JM, Kuriakose BB, Alhazmi AH, Muthusamy K. Structural and functional insights on vitamin D receptor and CYP24A1 deleterious single nucleotide polymorphisms: A computational and pharmacogenomics perpetual approach. Cell Biochemistry and Function. 2021 Oct;39(7):874-85.
  • 8. Krasniqi E, Boshnjaku A, Wagner KH, Wessner B. Association between Polymorphisms in Vitamin D Pathway-Related Genes, Vitamin D Status, Muscle Mass and Function: A Systematic Review. Nutrients. 2021 Sep;13(9):3109.
  • 9. Divanoglou N, Komninou D, Stea EA, Argiriou A, Papatzikas G, Tsakalof A, Pazaitou-Panayiotou K, Georgakis MK, Petridou E. Association of Vitamin D Receptor Gene Polymorphisms with Serum Vitamin D Levels in a Greek Rural Population (Velestino Study). Lifestyle Genomics. 2021 Jun 17:1-0.
  • 10. Bao Q, Wang D, Zhang Y, Bao L, Jia H. The impact of CYP24A1 polymorphisms on hypertension susceptibility. Kidney and Blood Pressure Research. 2020;45(1):28-37.
  • 11. Neves JPR , Silva AS, Morais LC, Diniz AS, Costa MJ, et al. Concentrações de 25-hidroxivitamina D e níveis pressóricos em idosos hipertensos. Arq Bras Endocrinol Metabol. 2012; 56(7):415-22. PMID:23108745
  • 12. Milazzo V, De Metrio M, Cosentino N, Marenzi G, Tremoli E, Vitamin D and acute myocardial infarction. Word J Cardiol. 2017;;9(1):14-20. Doi: 10.4330/wje.v9.i1.14
  • 13. Qian P, Cao X, Xu X, Duan M, Zhang Q, Huang G. Contribution of CYP24A1 variants in coronary heart disease among the Chinese population. Lipids in health and disease. 2020 Dec;19(1):1-7.
  • 14. Van Ballegooijen AJ, Kestenbaum B, Sachs MC, de Boer IH, Siscovick DS, Hoofenagle AN, et al. Association of 25-hydroxyvitamin D and parathyroid hormone with incident hypertension: MESA (Multi-Ethnic Study of Atherosclerosis). J Am Coll Cardiol. 2014; 63(12):1214-22.
  • 15. De Metrio M, Milazzo V, Rubino M, Cabiesti A Moltrasio M, Marana I, et al. Vitamin D plasma levels and in-hospital and 1-year outcomes in acute coronary syndromes: a prospective study. Medicine(Baltimore). 2015;94(19):e857
  • 16. Lee JH, Gadi R, Spertus JA, Tang F,O’Keefe JH. Prevalence of vitamin D deficiency in patients with acute myocardial infarction. Am J Cardiol. 2011;107(11):1636-8. Doi:10.1016/j.amjcard.2011.01.048 [ Links ]
  • 17. Milazzo V, De Metrio M, Cosentino N, Marenzi G, Tremoli E, Vitamin D and acute myocardial infarction. Word J Cardiol. 2017;;9(1):14-20. Doi: 10.4330/wje.v9.i1.14 [ Links ]
  • 18. Pandit A, Mookadam F, Boddu S, Aryal Pandit A, Tandar A, Chaliki H, et al. Vitamin D levels and left ventricular diastolic function. 2014. Open Heart. 2014;1(1):e000011 Doi: 10.1136/opener-2013-000011.eCollection2014 [ Links ]
  • 19. Kouvari M, Panagiotakos DB, Chrysohoou C, Yannakoulia M, Georgousopoulou EN, Tousoulis D, Pitsavos C, ATTICA study Investigators. Dietary vitamin D intake, cardiovascular disease, and cardiometabolic risk factors: a sex‐based analysis from the ATTICA cohort study. Journal of Human Nutrition and Dietetics. 2020 Oct;33(5):708-17.
  • 20. Latic N, Erben RG. Vitamin D and cardiovascular disease, with emphasis on hypertension, atherosclerosis, and heart failure. International Journal of Molecular Sciences. 2020 Jan;21(18):6483.
  • 21. Gouni-Berthold I, Berthold HK. Vitamin D and vascular disease. Current vascular pharmacology. 2021 May 1;19(3):250-68.
  • 22. Rodriguez AJ, Mousa A, Ebeling PR, Scott D, De Courten B. Effects of vitamin D supplementation on inflammatory markers in heart failure: a systematic review and meta-analysis of randomized controlled trials. Scientific reports. 2018 Jan 18;8(1):1-8.
  • 23. Gouni-Berthold I, Berthold HK. Vitamin D and vascular disease. Current vascular pharmacology. 2021 May 1;19(3):250-68
  • 24. Kim DH, Meza CA, Clarke H, Kim JS, Hickner RC. Vitamin D and endothelial function. Nutrients. 2020 Feb;12(2):575.
Yıl 2022, Cilt: 39 Sayı: 2, 497 - 502, 18.03.2022

Öz

Proje Numarası

12/2016

Kaynakça

  • 1. Jorge A, Cordeiro J, Rosa M, Bianchi D. Vitamin D Deficiency and Cardiovascular Diseases. International Journal of Cardiovascular Sciences. 2018;31(4):2
  • 2. Latic N, Erben RG. Vitamin D and cardiovascular disease, with emphasis on hypertension, atherosclerosis, and heart failure. International Journal of Molecular Sciences. 2020 Jan;21(18):6483.
  • 3. Riaz H, Finlayson A, Bashir S, Hussain S, Mahmood S, Malik F et al. Prevalence of Vitamin D deficiency in Pakistan and implications for the future. Expert Review of Clinical Pharmacology. 2016;9(2):329-338.
  • 4. Krist AH, Davidson KW, Mangione CM, Cabana M, Caughey AB, Davis EM, Donahue KE, Doubeni CA, Epling JW, Kubik M, Li L. Screening for Vitamin D Deficiency in Adults: US Preventive Services Task Force Recommendation Statement. JAMA. 2021 Apr 13;325(14):1436-42.
  • 5. de la Guía-Galipienso F, Martínez-Ferran M, Vallecillo N, Lavie CJ, Sanchis-Gomar F, Pareja-Galeano H. Vitamin D and cardiovascular health. Clinical Nutrition. 2020 Dec 29.
  • 6. Klomp SD, Manson ML, Guchelaar HJ, Swen JJ. Phenoconversion of cytochrome P450 metabolism: a systematic review. Journal of Clinical Medicine. 2020 Sep;9(9):2890.
  • 7. Jayaraj JM, Kuriakose BB, Alhazmi AH, Muthusamy K. Structural and functional insights on vitamin D receptor and CYP24A1 deleterious single nucleotide polymorphisms: A computational and pharmacogenomics perpetual approach. Cell Biochemistry and Function. 2021 Oct;39(7):874-85.
  • 8. Krasniqi E, Boshnjaku A, Wagner KH, Wessner B. Association between Polymorphisms in Vitamin D Pathway-Related Genes, Vitamin D Status, Muscle Mass and Function: A Systematic Review. Nutrients. 2021 Sep;13(9):3109.
  • 9. Divanoglou N, Komninou D, Stea EA, Argiriou A, Papatzikas G, Tsakalof A, Pazaitou-Panayiotou K, Georgakis MK, Petridou E. Association of Vitamin D Receptor Gene Polymorphisms with Serum Vitamin D Levels in a Greek Rural Population (Velestino Study). Lifestyle Genomics. 2021 Jun 17:1-0.
  • 10. Bao Q, Wang D, Zhang Y, Bao L, Jia H. The impact of CYP24A1 polymorphisms on hypertension susceptibility. Kidney and Blood Pressure Research. 2020;45(1):28-37.
  • 11. Neves JPR , Silva AS, Morais LC, Diniz AS, Costa MJ, et al. Concentrações de 25-hidroxivitamina D e níveis pressóricos em idosos hipertensos. Arq Bras Endocrinol Metabol. 2012; 56(7):415-22. PMID:23108745
  • 12. Milazzo V, De Metrio M, Cosentino N, Marenzi G, Tremoli E, Vitamin D and acute myocardial infarction. Word J Cardiol. 2017;;9(1):14-20. Doi: 10.4330/wje.v9.i1.14
  • 13. Qian P, Cao X, Xu X, Duan M, Zhang Q, Huang G. Contribution of CYP24A1 variants in coronary heart disease among the Chinese population. Lipids in health and disease. 2020 Dec;19(1):1-7.
  • 14. Van Ballegooijen AJ, Kestenbaum B, Sachs MC, de Boer IH, Siscovick DS, Hoofenagle AN, et al. Association of 25-hydroxyvitamin D and parathyroid hormone with incident hypertension: MESA (Multi-Ethnic Study of Atherosclerosis). J Am Coll Cardiol. 2014; 63(12):1214-22.
  • 15. De Metrio M, Milazzo V, Rubino M, Cabiesti A Moltrasio M, Marana I, et al. Vitamin D plasma levels and in-hospital and 1-year outcomes in acute coronary syndromes: a prospective study. Medicine(Baltimore). 2015;94(19):e857
  • 16. Lee JH, Gadi R, Spertus JA, Tang F,O’Keefe JH. Prevalence of vitamin D deficiency in patients with acute myocardial infarction. Am J Cardiol. 2011;107(11):1636-8. Doi:10.1016/j.amjcard.2011.01.048 [ Links ]
  • 17. Milazzo V, De Metrio M, Cosentino N, Marenzi G, Tremoli E, Vitamin D and acute myocardial infarction. Word J Cardiol. 2017;;9(1):14-20. Doi: 10.4330/wje.v9.i1.14 [ Links ]
  • 18. Pandit A, Mookadam F, Boddu S, Aryal Pandit A, Tandar A, Chaliki H, et al. Vitamin D levels and left ventricular diastolic function. 2014. Open Heart. 2014;1(1):e000011 Doi: 10.1136/opener-2013-000011.eCollection2014 [ Links ]
  • 19. Kouvari M, Panagiotakos DB, Chrysohoou C, Yannakoulia M, Georgousopoulou EN, Tousoulis D, Pitsavos C, ATTICA study Investigators. Dietary vitamin D intake, cardiovascular disease, and cardiometabolic risk factors: a sex‐based analysis from the ATTICA cohort study. Journal of Human Nutrition and Dietetics. 2020 Oct;33(5):708-17.
  • 20. Latic N, Erben RG. Vitamin D and cardiovascular disease, with emphasis on hypertension, atherosclerosis, and heart failure. International Journal of Molecular Sciences. 2020 Jan;21(18):6483.
  • 21. Gouni-Berthold I, Berthold HK. Vitamin D and vascular disease. Current vascular pharmacology. 2021 May 1;19(3):250-68.
  • 22. Rodriguez AJ, Mousa A, Ebeling PR, Scott D, De Courten B. Effects of vitamin D supplementation on inflammatory markers in heart failure: a systematic review and meta-analysis of randomized controlled trials. Scientific reports. 2018 Jan 18;8(1):1-8.
  • 23. Gouni-Berthold I, Berthold HK. Vitamin D and vascular disease. Current vascular pharmacology. 2021 May 1;19(3):250-68
  • 24. Kim DH, Meza CA, Clarke H, Kim JS, Hickner RC. Vitamin D and endothelial function. Nutrients. 2020 Feb;12(2):575.
Toplam 24 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Sağlık Kurumları Yönetimi
Bölüm Clinical Research
Yazarlar

Irfan Afzal Mughal Bu kişi benim 0000-0003-1093-626X

Noor-ul-ain Irfan Bu kişi benim 0000-0002-8503-1417

Saira Safdar Bu kişi benim

Sadia Moazzam Bu kişi benim

Asma Irfan 0000-0003-1093-626X

Amna Farooqi Bu kişi benim

Muhammad Ismail Bu kişi benim

Proje Numarası 12/2016
Erken Görünüm Tarihi 18 Mart 2022
Yayımlanma Tarihi 18 Mart 2022
Gönderilme Tarihi 15 Aralık 2021
Kabul Tarihi 27 Şubat 2022
Yayımlandığı Sayı Yıl 2022 Cilt: 39 Sayı: 2

Kaynak Göster

APA Afzal Mughal, I., Irfan, N.-u.-a., Safdar, S., Moazzam, S., vd. (2022). Association of CYP24A1 gene, Vitamin D deficiency and heart diseases in Pakistani patients. Journal of Experimental and Clinical Medicine, 39(2), 497-502.
AMA Afzal Mughal I, Irfan Nua, Safdar S, Moazzam S, Irfan A, Farooqi A, Ismail M. Association of CYP24A1 gene, Vitamin D deficiency and heart diseases in Pakistani patients. J. Exp. Clin. Med. Mart 2022;39(2):497-502.
Chicago Afzal Mughal, Irfan, Noor-ul-ain Irfan, Saira Safdar, Sadia Moazzam, Asma Irfan, Amna Farooqi, ve Muhammad Ismail. “Association of CYP24A1 Gene, Vitamin D Deficiency and Heart Diseases in Pakistani Patients”. Journal of Experimental and Clinical Medicine 39, sy. 2 (Mart 2022): 497-502.
EndNote Afzal Mughal I, Irfan N-u-a, Safdar S, Moazzam S, Irfan A, Farooqi A, Ismail M (01 Mart 2022) Association of CYP24A1 gene, Vitamin D deficiency and heart diseases in Pakistani patients. Journal of Experimental and Clinical Medicine 39 2 497–502.
IEEE I. Afzal Mughal, “Association of CYP24A1 gene, Vitamin D deficiency and heart diseases in Pakistani patients”, J. Exp. Clin. Med., c. 39, sy. 2, ss. 497–502, 2022.
ISNAD Afzal Mughal, Irfan vd. “Association of CYP24A1 Gene, Vitamin D Deficiency and Heart Diseases in Pakistani Patients”. Journal of Experimental and Clinical Medicine 39/2 (Mart 2022), 497-502.
JAMA Afzal Mughal I, Irfan N-u-a, Safdar S, Moazzam S, Irfan A, Farooqi A, Ismail M. Association of CYP24A1 gene, Vitamin D deficiency and heart diseases in Pakistani patients. J. Exp. Clin. Med. 2022;39:497–502.
MLA Afzal Mughal, Irfan vd. “Association of CYP24A1 Gene, Vitamin D Deficiency and Heart Diseases in Pakistani Patients”. Journal of Experimental and Clinical Medicine, c. 39, sy. 2, 2022, ss. 497-02.
Vancouver Afzal Mughal I, Irfan N-u-a, Safdar S, Moazzam S, Irfan A, Farooqi A, Ismail M. Association of CYP24A1 gene, Vitamin D deficiency and heart diseases in Pakistani patients. J. Exp. Clin. Med. 2022;39(2):497-502.