Affordable and Simple Protocol for Immunofluorescence Staining of Insulin Secreting iGL Cell Line 2D and 3D

Yıl 2024, Cilt: 41 Sayı: 3, 590 - 594, 30.09.2024

Endin Stujanna , Sri Ningsih , Zahra Nurusshofa , Nurhabibah Putri , Takashi Yashiro , Wawang Sukarya

Öz

Immunofluorescence staining is a widely used technique to visualize the localization of specific proteins in cells. This protocol provides an affordable and simple method for immunofluorescence staining of the Insulin-GLase (iGL) cell line in both 2D and 3D culture conditions. We describe cost-effective alternatives for reagents and materials without compromising the quality of the results, such as the use of Bovine Serum Albumin (BSA) 3% as a blocking buffer instead of normal serum. Immunofluorescence staining on the iGL cell line is a valuable technique for visualizing the distribution and localization of target biomolecules. With careful consideration of cost-effective strategies and an understanding of the unique characteristics of the iGL cell line, it is possible to achieve reliable and informative immunofluorescence results even with budget constraints.

Anahtar Kelimeler

Cell culture, Immunofluorescence, iGL, 2D and 3D culture

Kaynakça

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