The polymorphism of catalase gene C-262T: Impact on ulcerative colitis

Year 2025, Volume: 42 Issue: 1, 43 - 47, 28.03.2025

Nafiseh Nesbat Mohammadi , Hasan Bağcı , Talat Ayyıldız , İbrahim Gören , Beytullah Yıldırım

Abstract

Ulcerative Colitis is a chronic inflammatory disease of the digestive tract. Reactive oxygen species (ROS) causes inflammation and are thought to play a role in the pathophysiology of Inflammatory Bowel Disease (IBD). Catalase, one of the antioxidant enzymes, decomposes hydrogen peroxide into oxygen and water and protects the cells from oxidative damage of reactive oxygen species. It has been reported that CAT gene promoter polymorphism has a protective effect on oxidative stress-related diseases. The aim of this study was to investigate the association of catalase C-262T polymorphism with ulcerative colitis and to determine whether CATC-262T polymorphism is a risk factor for the disease. We investigated 80 patients with ulcerative colitis and 90 controls in the Black Sea region in Turkey. After blood sampling, DNA was extracted from the peripheral blood, then we design 2 sets of primers for C and T alleles. The polymorphism was determined by using PCR technique and the statistical analysis was performed by Kolmogorov-Smirnow test. There were no significant association between frequencies of the CATC-262Ts of the case and the control groups and genotype frequencies were very similar (P=0.996). This is the first report in regard to the association of CATC-262T polymorphism with ulcerative colitis in the Black Sea region in the Turkish population. According to the results of this study there were no significant differences in the genotype distribution and allelic frequency between patients and control groups.

Keywords

Ulcerative colitis, Catalase, ROS, Allele frequency

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