Investigation of Some Biological Activities of Inula graveolens (L.) Desf Species from Turkey

Yıl 2023, , 110 - 119, 31.12.2023

Ramazan Mammadov , Bayram Kaya , İlayda Cansu Atıcı , Mehmet Özgür Atay

https://doi.org/10.54370/ordubtd.1240934

Öz

In this study, it was aimed to determine the phenolic and flavonoid content and different biological activities (antioxidant, enzyme inhibitor, anthelmintic) of the methanol extract of Inula graveolens (L.) Desf collected from Muğla (Turkey). As a result of the study, the total phenolic content was determined as 5.36±0.32 mg GAE/g, and the total flavonoid amount was determined as 3.49±0.05 mg QE/g extract equivalent. In the ß-carotene/linoleic acid method, the extract showed lower activity than the standard BHA used. The extract was determined to be equivalent to 4.28±0.24/0.47±0.03 mg TE/g extract in terms of copper and iron-reducing power capacity, respectively. Although the enzyme inhibitory activities of the extract increased with the increase in concentration, it was determined that it had lower activity than galantamine (89.41±0.05%) and kojic acid (73.93±0.10%) used as standard. Paralysis and death times of the extract at different concentrations (2.5,5,10,20 mg/mL) on Tubifex tubifex worms were determined. It was determined that the extract at high concentrations (20 mg/mL) exhibited an activity near that of andazole (10 mg/mL) used as a standard. According to these results, I. graveolens can be considered a good resource for the pharmaceutical industry due to its activities.

Anahtar Kelimeler

Inula graveolens, antioxidant, enzyme inhibitor, Tubifex tubifex

Destekleyen Kurum

TUBİTAK

Proje Numarası

1919B012102904

Türkiye’den Toplanan Inula graveolens (L.) Desf. Türünün Bazı Biyolojik Aktivitelerinin Araştırılması

Öz

Bu çalışmada Muğla'dan toplanan Inula graveolens (L.) Desf türünün metanol ekstraktının fenolik ve flavonoid miktarı ve farklı biyolojik aktivitelerinin (antioksidan, enzim inhibitör, antihelmint) belirlenmesi amaçlanmıştır. Çalışma sonucunda total fenolik miktarı 5,36±0,32 mg GAE/g, total flavonoid miktarı ise 3,49±0,05 mg QE/g ekstrakt eşdeğeri olarak belirlenmiştir. ß-karoten/linoleik asit yönteminde ekstrakt standart olarak kullanılan BHA’ya göre daha düşük bir aktivite sergilemiştir. Ekstraktın bakır ve demir indirgeme gücü kapasitesi bakımından sırasıyla 4,28±0,24/0,47±0,03 mg TE/g ekstrakt eşdeğeri olduğu belirlenmiştir. Ekstraktın enzim inhibitör aktiviteleri konsantrasyon artışına bağlı artsa da standart olarak kullanılan galantamin (89.41±0.05%) ve kojik asite (73.93±0.10%) göre daha düşük bir aktiviteye sahip olduğu tespit edilmiştir. Farklı konsantrasyondaki ekstraktın (2.5,5,10,20 mg/mL) Tubifex tubifex solucanları üzerindeki paralize ve ölüm süreleri belirlenmiştir. Yüksek konsantrasyonlardaki ekstraktın (20 mg/mL) standart olarak kullanılan andazole (10 mg/mL) yakın bir aktivite sergilediği belirlenmiştir. Bu sonuçlara göre I. graveolens gösterdiği aktivitelerden dolayı ilaç endüstrisi için iyi bir kaynak olarak dikkate alınabilir.

Anahtar Kelimeler

Inula graveolens, Tubifex tubifex, antioksidan, enzim inhibitör

Proje Numarası

1919B012102904

Kaynakça

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