Effect of Tryptanthrin Compound on Lipop olysa cchar ide-I nduce d Inflammation Model in L929 Cell Line

Abstract

Objective: Our aim is to evaluate the effects of the tryptanthrin compound, with antimicrobial and anti-inflammatory effects, on the lipopolysaccharide inflammation model created in the L929 cell line. Methods: L929 cell lines were placed in a 5% CO2 incubator at 37°C, using standard cell culture procedures, and lipopolysaccharide was applied with Prn pure substance with a final concentration of 2 μL and 1 μL after 1 hour. Following the lipopolysaccharide application, the (3- [4,5- dimethylthiazol- 2-yl] -2,5-diphenyl tetrazolium bromide protocol, a colorimetric method, was applied to determine cell viability at the end of the required 24th, 48th, and 72nd hours incubation times. Results: IC50 values were calculated as 0.3 μg/mL for tryptanthrin and 1 μg/mL for lipopolysaccharide . Application of lipopolysaccharide to L929 cell lines caused a significant decrease in cellular index depending on time. Also, in the tryptanthrin + lipopolysaccharide groups, it was found that the decreased cell index significantly increased even closer to the control compared to the lipopolysaccharide applied group. It was found that the inflammation and cell damage caused by lipopolysaccharide applied to the L929 cell line improved after tryptanthrin application. Conclusion: When we look at the results of our study as a whole, it was determined that the viability rates of the cells were above 85% as a result of the tryptanthrin application and it was significantly above the 70% viable cell ratio recommended by ISO 10993-5: 2009 Dın en ISO (2009) for cytotoxicity.

Keywords

• Inflammation
• L929
• LPS
• MTT
• Tryptanthrin

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