Electrochemical biosensor designing for investigation of Ciprofloxacine

Abstract

Objective: Quinolone antibiotics deserve careful research due to their unique effects on bacteria and viruses. This will form the basis for synthesizing new generations of drugs that could prevent the impending human deaths caused by antibiotic resistance. For this purpose, in this study we designed a comparison method for the interaction mechanism of DNA and quinolone antibiotics. The effects of Ciprofloxacine (CIP) on different DNA sequences were studied electrochemically. Methods: The electrochemical DNA-based biosensor was designed for interaction between fluoroquinolone antibiotic (CIP) and DNA (dsDNA and ssDNA) sequences by using a pencil graphite electrode (PGE) with voltammetry technique. Consequently, the developed biosensor provides a suitable stage for the analysis of CIP-DNA interaction sensitively. Results: In the recent study, the interaction of the antibacterial drug was investigated with the DNA-modified electrodes. The binding mechanism of the antibacterial drug (CIP) with dsDNA and ssDNA was determined by using the electrochemical method. for this purpose, DNA-modified electrodes were prepared and interacted with 5 µM CIP, then measured by electrochemical analysis. Conclusion: In the present study, the binding effect of the antibacterial drug obtained from two different methods on DNA was examined together.

Keywords

• DNA
• Quinolone antibiotics
• Biosensor

Siprofloksasin araştırmaları için elektrokimyasal biyosensör tasarımı

Öz

Amaç: Kinolon antibiyotikler, bakteri ve virüsler üzerindeki benzersiz etkileri nedeniyle dikkatli bir araştırmayı hak etmektedir. Bu, antibiyotik direncinin neden olduğu yaklaşan insan ölümlerini önleyebilecek yeni nesil ilaçların sentezlenmesinin temelini oluşturacaktır. Bu amaçla, bu çalışmada DNA ve kinolon antibiyotiklerinin etkileşim mekanizması için bir karşılaştırma yöntemi tasarladık. Siprofloksasinin (CIP) farklı DNA dizileri üzerindeki etkileri elektrokimyasal olarak incelendi. Yöntemler: Elektrokimyasal DNA tabanlı biyosensör, voltametri tekniği kullanılarak bir kalem grafit elektrot (PGE) kullanılarak florokinolon antibiyotik (CIP) ve DNA (dsDNA ve ssDNA) dizileri arasındaki etkileşim için tasarlandı. Sonuç olarak, geliştirilen biyosensör, CIP-DNA etkileşiminin hassas bir şekilde analiz edilmesi için uygun bir ortam sunmaktadır. Sonuçlar: Son çalışmada, antibakteriyel ilacın DNA modifiye elektrotlarla etkileşimi araştırılmıştır. Antibakteriyel ilacın (CIP) dsDNA ve ssDNA ile bağlanma mekanizması elektrokimyasal yöntem kullanılarak belirlendi. Bu amaçla, DNA modifiye elektrotlar hazırlandı ve 5 µM CIP ile etkileşime sokuldu, ardından elektrokimyasal analizle ölçüldü. Bu çalışmada, iki farklı yöntemle elde edilen antibakteriyel ilacın DNA üzerindeki bağlanma etkisi birlikte incelendi.

Anahtar Kelimeler

• DNA
• Kinolon Antibiyotikleri
• Biyosensör

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