The Role of Insulin Receptor Substrate 4 (IRS4) Protein in the Radiotherapy Response of Glioblastoma Multiforme (GBM) Cells

Year 2024, Volume: 4 Issue: 4, 102 - 106, 10.10.2024

Aysun Arslan , Tahir Çakir Gökhan Görgişen

https://doi.org/10.5281/zenodo.13880181

Abstract

Objective: To explain the fundamental role of Insulin Receptor Substrate 4 (IRS4) protein in the response of Glioblastoma Multiforme (GBM) cells to radiotherapy.
Methods: LN229 cells were transfected with IRS4 expression vector using lipofectamine, and the ectopic IRS4 expression was confirmed by western blot. After irradiating LN229 cells with 5, 8, and 10 Gy doses of radiotherapy, the functional effect of IRS4 on radiotherapy was determined using MTT and colony formation assays.
Results: It was determined that increased IRS4 expression led to enhanced radiosensitivity in GBM cells. Increased IRS4 expression in the GBM cell line was found to cause a decrease in cell survival rates and colony formation rates.
Conclusion: IRS4 has been identified to potentially play an active role in the radiotherapy response of GBM cells.

Keywords

IRS4, GBM, Radiotherapy

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