The Antimicrobial Activity of Hippophae Rhamnoides

Abstract

Hippophae rhamnoides is a spiny decidous shrub. The plant has sour sweet little fruits ranging from yellow to red. Although it is edible, it is traditionally used fort he treatment of stomach and intestines by consuming these fruits in the form of tea fort he treatment of wounds by using it in the form marmalade. Fort his purpose we started to investigate whether the plants used in traditional medicine in Erzurum and its vicinity have antibiotic effects. Among them we investigated the fruits of Hippophae Rhamnoides, which is called as ‘’yalancı iğde’’ locally and grown in abundance in the Oltu-Tortum districts of Erzurum, Turkey. In the study, it was aimed to determine the antimicrobial activities of aqueous extraction of fruits. Fort he purpose, Escherchia coli, Pseudomonas aureginosa, Staphylococcus aureus and Candida albicans microorganisms were used as reference and the antimicrobial activities of fruit compared with gentamiycin (10ug) using disc diffusion method, As a negative control, DMSO used. According in the findings gentamiycin diameter of 20 mm was found an all samples,while Escherchia coli 11 mm, Pseudomonas aureginosa 10 mm, Staphylococcus aureus 15 mm zone diameters were observed in the aqueous extraction of fruit and there was no effect for Candida albicans. As a result, it is thought that it may be an alternative to skin infections because it has a higher antimicrobial effect on Staphylococcus aureus which fraquently causes skin infectious.

Keywords

• H. rhamnoides
• Antimicrobial Activity
• Disc Diffusion Method

References

1. 1- Arısoy, E.S. 2011. Gram negatif hastane enfeksiyonlarının sağaltımı, Journal of pediatric infection, vol. 5, #1, 152-156.
2. 2- Baştürk, S. 2005. Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa ve Acinetobacter baumannii suşlarında çeşitli kinolon grubu antibiyotiklerin duyarlılıklarının araştırılması, Uzmanlık tezi, T.C. sağlık bakanlığı haseki eğitim ve araştırma hastanesi enfeksiyon hastalıkları ve klinik mikrobiyoloji kliniği.
3. 3- Bexfield, A., Nigam, Y., Thomas, S., Ratcliffe, N.A. 2004. Detection and partial characterisation of two antibacterial factors from the excretions/secretions of the medicinal maggot Lucilia sericata and their activity against methicillin-resistant Staphylococcus aureus (MRSA), Microbes Infect, vol.,16 #4, 1297-304.
4. 4- Chawla, R., Arora, R., Singh, S., Sagar, R. K., Sharma, R. K., Kumar, R. et al. 2007, Radioprotective and antioxidant activity of fractionated extracts of berries of Hippophae rhamnoides, Journal of Medicinal Food, vol., #10, 101–9.
5. 5- Conner, D.E. 1993. Naturally occurring compounds, antimicrobials in foods, In: Davidson, P.M., Branen, A.L. (ed.)., Marcel Dekker, New York, 441-68.
6. 6- Kaushal, M., Sharma, P.C., 2011. Nutritional and antimicrobial property of sea buckthorn (Hippophae sp.) seed oil, J Sci Indust Res, vol., #70, 1033-6.
7. 7- Sadowska, B., Budzyńska, A., Stochmal, A., Żuchowski, J., & Różalska, B. 2017. Novel properties of Hippophae rhamnoides L. twig and leaf extracts-anti-virulence action and synergy with antifungals studied in vitro on Candida spp. model. Microbial pathogenesis,vol., # 107, 372-9.
8. 8- Saleem, M., Nazir, M., Ali, M.S., Hussain, H., Lee, Y.S., Riaz, N., Jabbar, A., 2010. Antimicrobial natural products: An update on future antibiotic drug candidates, Natural product reports, vol., #27, 2, 238-54.

9. 9- Aras, A., Ceylan, F.D., Yanar, O., Boztas, K., Capanoglu, E., 2016. Investigating the antioxidant properties and rutin content of sea buckthorn (Hippophae rhamnoides L.) leaves and branches, African Journal of Biotechnology, vol., #15, 5, 118-24.
10. 10- Gupta, S.M., Gupta, A.K., Ahmed, Z, Kumar, A. 2011. Antibacterial and Antifungal Activity in Leaf, Seed Extract and Seed Oil of Seabuckthorn (Hippophae salicifolia D. Don) Plant. J Plant Pathol Microbiol vol., #2:105.
11. 11- Qadir, M. I., Abbas, K., Younus, A., & Shaikh, R. S. 2016. Antibacterial activity of sea buckthorn (Hippophae rhamnoides L.) against methicillin resistant Staphylococcus aureus (MRSA). Pak J Pharm Sci, vol., #29,5, 1705-7.