Evaluation of antioxidant prophylactic effects of parsley (Petroselinum crispum) on sepsis following cecal ligation and puncture
Yıl 2024,
Cilt: 2 Sayı: 2, 58 - 66, 29.08.2024
Furkan Çağan
,
İhsan Kulaksiz
,
Nigar Vardı
,
Hakan Parlakpınar
,
Onural Özhan
,
Azibe Yıldız
,
Alaadin Polat
,
Kevser Tanbek
,
Ahmet Kadir Arslan
,
Yahya Atayan
Öz
Objective: Sepsis causes the release of free oxygen radicals that disrupt membrane integrity, and damage to Mitochondria due to the production of free oxygen radicals and oxidation leads to exacerbation of sepsis, Cecal ligation and puncture (CLP) in rats mimics the characteristics and course of clinical sepsis (Hubbard et al., 2005).
Methods: We evaluated the antioxidant effects of Petroselinum crispum (Pc) in a cecal ligation and puncture (CLP)-induced rat sepsis model, in a rat model of sepsis caused by cecal ligation and puncture (CLP). Wistar albino rats were separated into four groups of eight groups: a sham group with incised and sutured abdomens; a Pc extract (PcE) group, which was given 2 g/kg parsley extract for 14 days by gastric gavage; a CLP group, which was subjected to sepsis caused by CLP; and a PcE + CLP group, which was given parsley extract for 14 days. PcE was given for 14 days, after which sepsis was induced via the CLP procedure. The groups were compared in terms of hemogram, biochemical and histological characteristics.
Results: The administration of PcE before CLP-induced sepsis increases neutrophil counts, PLTs and TASs, which decrease with sepsis, and decreases biochemical changes (BUN, AST, ALT, LDH, TOS, and OSI), which increase with sepsis, to protect against sepsis. Compared with that in the CLP group, the severity of intestinal infiltration was significantly lower in the PcE + CLP group; however, the degree of epithelial damage in the PcE + CLP group was similar to that in the CLP group. In the PcE + CLP group, the crypt and villus lengths were greater, and the decrease in Paneth cell degranulation intensity was greater than that in the CLP group.
Conclusion: Additionally, the morphology of the cells in the PcE + CLP group was similar to that in the sham group. PcE has potential as a prophylactic agent for sepsis.
Etik Beyan
The Ethics Committee for Experimental Animal Research approved our study (Reference no. 2015/A-34).
Kaynakça
- Adolph, T. E., Tomczak, M. F., Niederreiter, L., Ko, H.-J., Böck, J., Martinez-Naves, E., Glickman, J. N., Tschurtschenthaler, M., Hartwig, J., & Hosomi, S. (2013). Paneth cells as a site of origin for intestinal inflammation. Nature, 503(7475), 272–276.
- Al-Juhaimi, F., & Ghafoor, K. (2011). Total phenols and antioxidant activities of leaf and stem extracts from coriander, mint and parsley grown in Saudi Arabia. Pakistan Journal of Botany, 43(5), 2235–2237.
- Bancroft, J. D., & Gamble, M. (2008). Theory and practice of histological techniques. Elsevier Health Sciences.
Bone, R. C. (1991). The pathogenesis of sepsis. Annals of Internal Medicine, 115(6), 457–469.
- Cohen, J. (2002). The immunopathogenesis of sepsis. Nature, 420(6917), 885–891.
- Erel, O. (2004a). A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clinical Biochemistry, 37(4), 277–285.
- Erel, O. (2004b). A novel automated method to measure total antioxidant response against potent free radical reactions. Clinical Biochemistry, 37(12), 112–119.
- Erel, O. (2005). A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry, 38(12), 1103–1111.
- Farzaei, M. H., Abbasabadi, Z., Ardekani, M. R. S., Rahimi, R., & Farzaei, F. (2013). Parsley: A review of ethnopharmacology, phytochemistry and biological activities. Journal of Traditional Chinese Medicine, 33(6), 815–826.
- Fejes, S., Blazovics, A., Lemberkovics, E., Petri, G., Szöke, E., & Kery, A. (2000). Free radical scavenging and membrane protective effects of methanol extracts from Anthriscus cerefolium L. (Hoffm.) and Petroselinum crispum (Mill.) Nym. ex AW Hill. Phytotherapy Research, 14(5), 362–365.
- Hubbard, W. J., Choudhry, M., Schwacha, M. G., Kerby, J. D., Rue III, L. W., Bland, K. I., & Chaudry, I. H. (2005). Cecal ligation and puncture. Shock, 24(1), 52–57.
- Iwashyna, T. J., Netzer, G., Langa, K. M., & Cigolle, C. (2012). Spurious inferences about long-term outcomes: The case of severe sepsis and geriatric conditions. American Journal of Respiratory and Critical Care Medicine, 185(8), 835–841.
- Jones, A. E., Brown, M. D., Trzeciak, S., Shapiro, N. I., Garrett, J. S., Heffner, A. C., & Kline, J. A. (2008). The effect of a quantitative resuscitation strategy on mortality in patients with sepsis: A meta-analysis. Critical Care Medicine, 36(8), 2734–2741.
- Koksal, G., Sayilgan, C., Aydin, S., Oz, H., & Uzun, H. (2004). Correlation of plasma and tissue oxidative stresses in intra-abdominal sepsis. Journal of Surgical Research, 122(2), 180–183.
- Leng, Y., Zhang, K., Fan, J., Yi, M., Ge, Q., Chen, L., Zhang, L., & Yao, G. (2014). Effect of acute, slightly increased intra-abdominal pressure on intestinal permeability and oxidative stress in a rat model. PLoS ONE, 9(11), e109350.
Martin, G. S., Mannino, D. M., Eaton, S., & Moss, M. (2003). The epidemiology of sepsis in the United States from 1979 through 2000. New England Journal of Medicine, 348(16), 1546–1554.
- Neviere, R., Parsons, P. E., & Finlay, G. (2017). Sepsis syndromes in adults: Epidemiology, definitions, clinical presentation, diagnosis, and prognosis. In Monografía en internet (Wolters Kluwer: UpToDate).
- Ozsoy-Sacan, O., Yanardag, R., Orak, H., Ozgey, Y., Yarat, A., & Tunali, T. (2006). Effects of parsley (Petroselinum crispum) extract versus glibornuride on the liver of streptozotocin-induced diabetic rats. Journal of Ethnopharmacology, 104(1–2), 175–181.
- Parlakpinar, H., Ozhan, O., Ermis, N., Vardi, N., Cigremis, Y., Tanriverdi, L. H., Colak, C., & Acet, A. (2019). Acute and subacute effects of low versus high doses of standardized Panax ginseng extract on the heart: An experimental study. Cardiovascular Toxicology, 19(4), 306–320.
- Popović, M., Kaurinović, B., Jakovljević, V., Mimica-Dukic, N., & Bursać, M. (2007). Effect of parsley (Petroselinum crispum (Mill.) Nym. ex AW Hill, Apiaceae) extracts on some biochemical parameters of oxidative stress in mice treated with CCl4. Phytotherapy Research, 21(8), 717–723.
- Reinhart, K., Bloos, F., & Brunkhorst, F. (2005). Pathophysiology of sepsis and multiple organ dysfunction. In Textbook of Critical Care (5th ed., pp. 1249–1258). Elsevier-Saunders.
- Ritter, C., Andrades, M. E., Reinke, A., Menna-Barreto, S., Moreira, J. C. F., & Dal-Pizzol, F. (2004). Treatment with N-acetylcysteine plus deferoxamine protects rats against oxidative stress and improves survival in sepsis. Critical Care Medicine, 32(2), 342–349.
- Rodríguez, F., Barrera, L., De La Rosa, G., Dennis, R., Dueñas, C., Granados, M., Londoño, D., Molina, F., Ortiz, G., & Jaimes, F. (2011). The epidemiology of sepsis in Colombia: A prospective multicenter cohort study in ten university hospitals. Critical Care Medicine, 39(7), 1675–1682.
- Sęczyk, Ł., Świeca, M., Gawlik-Dziki, U., Luty, M., & Czyż, J. (2016). Effect of fortification with parsley (Petroselinum crispum Mill.) leaves on the nutraceutical and nutritional quality of wheat pasta. Food Chemistry, 190, 419–428.
- Shukla, S., & Gupta, S. (2010). Apigenin: A promising molecule for cancer prevention. Pharmaceutical Research, 27(5), 962–978.
- Taslidere, E., Vardi, N., Parlakpinar, H., Yıldız, A., Taslidere, B., & Karaaslan, M. (2018). Effects of melatonin on acetylsalicylic acid-induced gastroduodenal and jejunal mucosal injury. Biotechnic & Histochemistry, 93(6), 485–495.
- Toscano, M. G., Ganea, D., & Gamero, A. M. (2011). Cecal ligation puncture procedure. Journal of Visualized Experiments: JoVE, 54, e2824.
- Walley, K. R., Lukacs, N. W., Standiford, T. J., Strieter, R. M., & Kunkel, S. L. (1996). Balance of inflammatory cytokines related to severity and mortality of murine sepsis. Infection and Immunity, 64(11), 4733–4738.
- Wong, P. Y., & Kitts, D. D. (2006). Studies on the dual antioxidant and antibacterial properties of parsley (Petroselinum crispum) and cilantro (Coriandrum sativum) extracts. Food Chemistry, 97(4), 505–515.
- Zhang, H., Chen, F., Wang, X., & Yao, H.-Y. (2006). Evaluation of antioxidant activity of parsley (Petroselinum crispum) essential oil and identification of its antioxidant constituents. Food Research International, 39(7), 833–839.
- Zhou, L., Gao, M., Xiao, Z., Zhang, J., Li, X., & Wang, A. (2015). Protective effect of astaxanthin against multiple organ injury in a rat model of sepsis. Journal of Surgical Research, 195(2), 559–567.
Yıl 2024,
Cilt: 2 Sayı: 2, 58 - 66, 29.08.2024
Furkan Çağan
,
İhsan Kulaksiz
,
Nigar Vardı
,
Hakan Parlakpınar
,
Onural Özhan
,
Azibe Yıldız
,
Alaadin Polat
,
Kevser Tanbek
,
Ahmet Kadir Arslan
,
Yahya Atayan
Kaynakça
- Adolph, T. E., Tomczak, M. F., Niederreiter, L., Ko, H.-J., Böck, J., Martinez-Naves, E., Glickman, J. N., Tschurtschenthaler, M., Hartwig, J., & Hosomi, S. (2013). Paneth cells as a site of origin for intestinal inflammation. Nature, 503(7475), 272–276.
- Al-Juhaimi, F., & Ghafoor, K. (2011). Total phenols and antioxidant activities of leaf and stem extracts from coriander, mint and parsley grown in Saudi Arabia. Pakistan Journal of Botany, 43(5), 2235–2237.
- Bancroft, J. D., & Gamble, M. (2008). Theory and practice of histological techniques. Elsevier Health Sciences.
Bone, R. C. (1991). The pathogenesis of sepsis. Annals of Internal Medicine, 115(6), 457–469.
- Cohen, J. (2002). The immunopathogenesis of sepsis. Nature, 420(6917), 885–891.
- Erel, O. (2004a). A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clinical Biochemistry, 37(4), 277–285.
- Erel, O. (2004b). A novel automated method to measure total antioxidant response against potent free radical reactions. Clinical Biochemistry, 37(12), 112–119.
- Erel, O. (2005). A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry, 38(12), 1103–1111.
- Farzaei, M. H., Abbasabadi, Z., Ardekani, M. R. S., Rahimi, R., & Farzaei, F. (2013). Parsley: A review of ethnopharmacology, phytochemistry and biological activities. Journal of Traditional Chinese Medicine, 33(6), 815–826.
- Fejes, S., Blazovics, A., Lemberkovics, E., Petri, G., Szöke, E., & Kery, A. (2000). Free radical scavenging and membrane protective effects of methanol extracts from Anthriscus cerefolium L. (Hoffm.) and Petroselinum crispum (Mill.) Nym. ex AW Hill. Phytotherapy Research, 14(5), 362–365.
- Hubbard, W. J., Choudhry, M., Schwacha, M. G., Kerby, J. D., Rue III, L. W., Bland, K. I., & Chaudry, I. H. (2005). Cecal ligation and puncture. Shock, 24(1), 52–57.
- Iwashyna, T. J., Netzer, G., Langa, K. M., & Cigolle, C. (2012). Spurious inferences about long-term outcomes: The case of severe sepsis and geriatric conditions. American Journal of Respiratory and Critical Care Medicine, 185(8), 835–841.
- Jones, A. E., Brown, M. D., Trzeciak, S., Shapiro, N. I., Garrett, J. S., Heffner, A. C., & Kline, J. A. (2008). The effect of a quantitative resuscitation strategy on mortality in patients with sepsis: A meta-analysis. Critical Care Medicine, 36(8), 2734–2741.
- Koksal, G., Sayilgan, C., Aydin, S., Oz, H., & Uzun, H. (2004). Correlation of plasma and tissue oxidative stresses in intra-abdominal sepsis. Journal of Surgical Research, 122(2), 180–183.
- Leng, Y., Zhang, K., Fan, J., Yi, M., Ge, Q., Chen, L., Zhang, L., & Yao, G. (2014). Effect of acute, slightly increased intra-abdominal pressure on intestinal permeability and oxidative stress in a rat model. PLoS ONE, 9(11), e109350.
Martin, G. S., Mannino, D. M., Eaton, S., & Moss, M. (2003). The epidemiology of sepsis in the United States from 1979 through 2000. New England Journal of Medicine, 348(16), 1546–1554.
- Neviere, R., Parsons, P. E., & Finlay, G. (2017). Sepsis syndromes in adults: Epidemiology, definitions, clinical presentation, diagnosis, and prognosis. In Monografía en internet (Wolters Kluwer: UpToDate).
- Ozsoy-Sacan, O., Yanardag, R., Orak, H., Ozgey, Y., Yarat, A., & Tunali, T. (2006). Effects of parsley (Petroselinum crispum) extract versus glibornuride on the liver of streptozotocin-induced diabetic rats. Journal of Ethnopharmacology, 104(1–2), 175–181.
- Parlakpinar, H., Ozhan, O., Ermis, N., Vardi, N., Cigremis, Y., Tanriverdi, L. H., Colak, C., & Acet, A. (2019). Acute and subacute effects of low versus high doses of standardized Panax ginseng extract on the heart: An experimental study. Cardiovascular Toxicology, 19(4), 306–320.
- Popović, M., Kaurinović, B., Jakovljević, V., Mimica-Dukic, N., & Bursać, M. (2007). Effect of parsley (Petroselinum crispum (Mill.) Nym. ex AW Hill, Apiaceae) extracts on some biochemical parameters of oxidative stress in mice treated with CCl4. Phytotherapy Research, 21(8), 717–723.
- Reinhart, K., Bloos, F., & Brunkhorst, F. (2005). Pathophysiology of sepsis and multiple organ dysfunction. In Textbook of Critical Care (5th ed., pp. 1249–1258). Elsevier-Saunders.
- Ritter, C., Andrades, M. E., Reinke, A., Menna-Barreto, S., Moreira, J. C. F., & Dal-Pizzol, F. (2004). Treatment with N-acetylcysteine plus deferoxamine protects rats against oxidative stress and improves survival in sepsis. Critical Care Medicine, 32(2), 342–349.
- Rodríguez, F., Barrera, L., De La Rosa, G., Dennis, R., Dueñas, C., Granados, M., Londoño, D., Molina, F., Ortiz, G., & Jaimes, F. (2011). The epidemiology of sepsis in Colombia: A prospective multicenter cohort study in ten university hospitals. Critical Care Medicine, 39(7), 1675–1682.
- Sęczyk, Ł., Świeca, M., Gawlik-Dziki, U., Luty, M., & Czyż, J. (2016). Effect of fortification with parsley (Petroselinum crispum Mill.) leaves on the nutraceutical and nutritional quality of wheat pasta. Food Chemistry, 190, 419–428.
- Shukla, S., & Gupta, S. (2010). Apigenin: A promising molecule for cancer prevention. Pharmaceutical Research, 27(5), 962–978.
- Taslidere, E., Vardi, N., Parlakpinar, H., Yıldız, A., Taslidere, B., & Karaaslan, M. (2018). Effects of melatonin on acetylsalicylic acid-induced gastroduodenal and jejunal mucosal injury. Biotechnic & Histochemistry, 93(6), 485–495.
- Toscano, M. G., Ganea, D., & Gamero, A. M. (2011). Cecal ligation puncture procedure. Journal of Visualized Experiments: JoVE, 54, e2824.
- Walley, K. R., Lukacs, N. W., Standiford, T. J., Strieter, R. M., & Kunkel, S. L. (1996). Balance of inflammatory cytokines related to severity and mortality of murine sepsis. Infection and Immunity, 64(11), 4733–4738.
- Wong, P. Y., & Kitts, D. D. (2006). Studies on the dual antioxidant and antibacterial properties of parsley (Petroselinum crispum) and cilantro (Coriandrum sativum) extracts. Food Chemistry, 97(4), 505–515.
- Zhang, H., Chen, F., Wang, X., & Yao, H.-Y. (2006). Evaluation of antioxidant activity of parsley (Petroselinum crispum) essential oil and identification of its antioxidant constituents. Food Research International, 39(7), 833–839.
- Zhou, L., Gao, M., Xiao, Z., Zhang, J., Li, X., & Wang, A. (2015). Protective effect of astaxanthin against multiple organ injury in a rat model of sepsis. Journal of Surgical Research, 195(2), 559–567.