C. COGGYGRİA SCOP. YAPRAKLARINDAN ELDE EDİLEN SULU İNFÜZYONUN DEKSTRAN SODYUM SÜLFAT (DSS) İLE İNDÜKLENMİŞ ÜLSERATİF KOLİTLİ FARELERDE HEPATİK OKSİDATİF STRES ÜZERİNE ETKİSİ

Abstract

Amaç: Serbest radikaller ülseratif kolit (ÜK) ile ilişkili karaciğer bulguların da önemli bir rol oynadığından, bitki kökenli antioksidanlar karaciğer hasarına karşı potansiyel terapötik ajanlar olarak önerilmiştir. Bu çalışma nın amacı, C. coggygria yapraklarının sulu infüzyonunun (CCLAI), DSS ile indüklenmiş ÜK’li farelerin karaciğerindeki oksidatif stresi hafifletmede mesalamine kıyasla iyileştirici etkisini aydınlatmaktır. Gereç ve Yöntemler: İki farklı dozda CCLAI (%4 ve %6) veya mesalamin (250 mg/kg vücut ağırlığı) C57BL/6 erkek farelere oral gavaj yoluyla ardışık 7 gün boyunca günde bir kez verildi. ÜK, sadece su verilen normal ve bitki kontrol grupları haricinde, 5 gün boyunca içme suyunda %3 DSS ile indüklenmiştir. Bulgular: Karaciğer hasarı belirteçleri olan AST ve ALT aktivitelerinde tüm gruplar arasında istatistiksel bir fark gözlenmemiştir, bu da oksidatif stres oluşmasına rağmen oksidatif değişikliklerin karaciğer hasarına neden olmak için yeterli olmadığını düşündürmektedir. Normal gruba kıyasla kolit gruplarında antioksidan belirteçlerin (SOD, CAT, GR, GPx, GST) aktivitelerinin ve GSH ile NO seviyelerinin artması, oksidatif strese karşı bir ilk savunma mekanizmasını temsil ediyor olabilir. Bu sonuçlar, MDA seviyeleri ve MPO aktivitesinin azalması ile birlikte oksidatif stres parametrelerinin normal kontrol değerlerine doğru gerilemesinin de gösterdiği gibi, CCLAI’nin oksidatif stresi azaltabileceğini düşündürmektedir. Sonuç: Çalışmamız, CCLAI’nin muhtemelen ROS’u temizleyerek ve hepatik dokulardaki oksidan/antioksidan dengeyi modüle ederek oksidatif stresi azaltabildiğine dair kanıtlar sağlamıştır.

Keywords

• Cotinus coggygria
• antioksidan aktivite
• dekstran sülfat sodyum
• karaciğer
• oksidatif stres
• ülseratif kolit

INFUSION FROM C. COGGYGRIA SCOP. LEAVES ON THE HEPATIC OXIDATIVE STRESS IN MICE WITH DEXTRAN SODIUM SULPHATE (DSS)-INDUCED ULCERATIVE COLITIS

Abstract

Objective: Since free radicals play a crucial role in ulcerative colitis (UC)- assotiated liver manifestations, antioxidants of plant origin have been proposed as potential therapeutic agents to counteract liver damage. This study aimed to elucidate the ameliorative effect of the aqueous infusion of the C. coggygria leaves (CCLAI) in comparison with a mesalamine in alleviating the oxidative stress in the liver of mice with DSS-induced UC. Material and Methods: Two different doses of CCLAI (4% and 6%) or mesalamine (250 mg/kg body weight) were administered by oral gavage to C57BL/6 male mice once a day for 7 consecutive days. UC was induced with 3% DSS in drinking water for 5 days, except the normal and plant control groups that had access to water only. Results: No statistical difference between all the groups was observed in the activities of AST and ALT, hepatic damage markers, suggesting that the oxidative alterations were not sufficient to cause liver damage, although oxidative stress occurred. The elevated activities of antioxidant markers (SOD, CAT, GR, GPx, GST) and increased GSH and NO levels in the colitis groups compared with the normal group may represent an initial defence mechanism against oxidative stress. These results indicate that CCLAI may attenuate oxidative stress, as demonstrated by decreased MDA levels and MPO activity and reversed levels of oxidative stress parameters towards the value of normal controls. Conclusion: Our study provided evidence that CCLAI can reduce oxidative stress probably by scavenging ROS and modulating the oxidant/antioxidant balance in hepatic tissues.

Keywords

• Cotinus coggygria
• antioxidant activity
• dextran sulphate sodium
• liver
• oxidative stress
• ulcerative colitis

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