TÜRKİYE POPÜLASYONUNDA HLA-DQ POLİMORFİZMLERİNİN HEPATİT B VİRÜS ENFEKSİYONU İLE İLİŞKİSİ

Yıl 2022, Cilt: 5 Sayı: 3, 186 - 192, 16.11.2022

Bülent Çakal , Muzaffer Arıkan , Alp Atasoy , Bilger Çavuş , Mehveş Poda , Mesut Bulakçı , Mine Güllüoğlu , Mehmet Demirci , Filiz Akyüz

https://doi.org/10.26650/JARHS2022-1142531

Öz

Amaç Konak genetik faktörleri hepatit B virüs (HBV) enfeksiyonunun doğal seyri ve HBV ilişkili karaciğer hastalıklarının gelişme riski ile progresyonu üzerinde etkili olabilmektedir. Bu çalışmada HLA-DQ gen rs9272105, rs2856718 ve rs9275572 polimorfizmlerinin HBV doğal klirensi, viral yük ve HBV ile ilişkili karaciğer hasarı gelişimi ile ilişkisinin değerlendirilmesi amaçlanmıştır. Gereç ve Yöntem Çalışmaya 150 kronik hepatit B (KHB) hastası ile kontrol grubu olarak 58’i kronik hepatit C (KHC) ve 82’si farklı klinik endikasyonlar nedeniyle karaciğer biyopsi işlemi gerçekleştirilen 140 hasta dâhil edildi. HLA-DQ rs9272105, rs2856718 ve rs9275572 genotip ve polimorfizmlerinin belirlenmesinde TaqMan SNP genotiplendirme yöntemi kullanıldı. Bulgular KHB’li ve kontrol grubundaki hastaların HLA-DQ gen rs9272105, rs2856718 ve rs9275572 genotip ve allel frekansları arasında farklılık tespit edildi (P<0,05). HLA-DQ rs9272105 AA genotip ve A allel varlığı, hepatit B yüzey antijen (Hepatitis B surface antigen; HBSAg) klirensi ve karaciğer hasarı ile ilişkiliydi (p<0,05). HLA-DQ gen rs2856718 ve rs9275572 ise HBV klirensi ve hastaların histolojik sonuçlarıyla ve ayrıca rs9272105 de dâhil hastaların viral yükleriyle ilişkili değildi. Sonuç HLA-DQ rs9272105 AA genotip ve A allel gerek HBV enfeksiyonunun kronikleşmesi gerekse HBV ilişkili karaciğer hasarının gelişmesi için risk faktördür.

Anahtar Kelimeler

Hepatit B virüs, HLA-DQ, genetik polimorfizm, serokonversiyon, hastalık progresyonu, Hepatitis B Virus, genetic polymorphism, seroconversion, disease progression

Destekleyen Kurum

Bu çalışma İstanbul Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından desteklenmiştir.

Proje Numarası

TSA-2018-30611

Teşekkür

İstanbul Tıp Fakultesi Gastroenterohepatoloji Bilim Dalında gorevli Sayın Hemşire Nilay ARABACI ve Derya KAYA ile Radyoloji Biriminde görevli Sayın Hemşire Arzu ÖRENTEL’e bu projenin gerçekleştirilmesi aşamasındaki bilimsel duyarlılıkları, katkıları ve emeklerinden dolayı şükranlarımı sunarım.

ASSOCIATION OF HLA-DQ POLYMORPHISMS WITH HEPATITIS B VIRUS INFECTION IN TURKISH POPULATION

Öz

Objective: Host genetic factors can affect the natural course of hepatitis B virus (HBV) infection and the risk of development and progression of HBV-related liver diseases. The aim of this study is to evaluate the role of the HLA-DQ gene polymorphisms rs9272105, rs2856718 and rs9275572 with HBV natural clearance, viral load and the development of HBV associated liver injury. Materials and Methods: The study included 150 patients with chronic hepatitis B (CHB) and 140 patients as the control group, 58 of whom had chronic hepatitis C (CHC) and 82 of whom had undergone a liver biopsy due to different clinical indications. The HLA-DQ gene rs9272105, rs2856718 and rs9275572 polymorphisms were genotypes in liver samples using the hybridization probe assay. Results: A difference was found between the HLA-DQ gene rs9272105, rs2856718 and rs9275572 genotype and allele frequencies of the patients with CHB and the control group (P<0,05). The HLA-DQ rs9272105 AA genotype and presence of A allele were associated with hepatitis B surface antigen (HBsAg) clearance and liver injury (p<0,05). In contrast, the HLA-DQ genes rs2856718 and rs9275572 were not associated with HBV clearance and patients’ histological outcomes, nor with patients’ viral load, including rs9272105. Conclusions: It has been suggested that the HLA-DQ rs9272105 AA genotype and the A allele are risk factors for both the persistence of HBV infection and the development of HBV-related liver damage. 

Anahtar Kelimeler

Hepatitis B Virus, genetic polymorphism, seroconversion, disease progression

Proje Numarası

TSA-2018-30611

Kaynakça

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