Farklı Omurgalı Türlerinde Bazı Sindirim Kanalı Bölgelerinin Histolojik ve Histokimyasal Yapısı

Year 2014, Volume: 9 Issue: 2, 61 - 70, 31.12.2014

Nurgül Şenol , Dilek Bayram , Özlem Yeşil

Abstract

In the present observation, histochemical methods were utilized to understand the structural organization and to categorize the diverse classes of glycoproteins eleborated by the epithelium in various regions of digestive tract in different vertebrate species (Mus musculus, Coturnix coturnix and Salmo trutta) In the study, two units adult 4-6 months old quail (Coturnix coturnix) were obtained from Suleyman Demirel University, Department of Animal Science poultry rearing, two trout (Salmo trutta) were provided from breeding pool, two mouse (Mus musculus) were provided from Suleyman Demirel University Experimental Animal Production and Experimental Research Laboratories. In trout, histochemical staining showed a positive reaction in all areas but especially reaction was more powerful in cardia and fundus region. Sulphated mucosubstances were intense in stomach and intestines in quail. In mouse, histochemical apllications were most strong in the lamina epitheliyalis andglands of all region. As a result of histochemical investigations, neutral mucosubstance were more intense than other mucosubstance in trout and mouse, but sulphated mucosubstances were intense in quail

Keywords

Coturnix coturnix, Glycoprotein, Histochemistry, Mus musculus, Salmo trutta

Farklı Omurgalı Türlerinde Bazı Sindirim Kanalı Bölgelerinin Histolojik ve Histokimyasal Yapısı

Abstract

Özet: Bu çalışmanın amacı, farklı omurgalı türlerinin (Mus musculus, Coturnix coturnix ve Salmo trutta) sindirim kanalının bazı bölgelerinin histolojik yapısı ve mukus hücrelerindeki glikoproteinlerinin histokimyasal tekniklerle belirlemektir. Çalışmada, Süleyman Demirel Üniversitesi Zootekni Bölümü Kanatlı Hayvan Yetiştirme biriminden 2 adet 4-6 aylık yetişkin bıldırcın (Coturnix coturnix), yetiştirme havuzundan 2 adet alabalık (Salmo trutta) ve Süleyman Demirel Üniversitesi Deney Hayvanı Üretimi ve Deneysel Araştırma Laboratuvarından 2 adet fare (Mus musculus) temin edildi. Alabalıkta uygulanan histokimyasal boyamaların tüm bölgelerde pozitif reaksiyon gösterdiği özelliklede kardiya ve fundus bölgesinde boyanmanın daha güçlü olduğu gözlendi. Bıldırcın mide ve bağırsaklarında sülfatlı mukosubstans içeriğinin fazla olduğu saptandı. Yapılan histokimyasal uygulamalarda farede lamina epitelyalis ve bezlerde boyanmaların çok güçlü olduğu tespit edildi. Sonuç olarak histokimyasal incelemelerde alabalık ve farede nötral mukosubstans içeriğinin, bıldırcında ise sülfatlı mukosubstans içeriğinin diğer mukosubstanslara göre biraz daha yoğun olduğu tespit edildi.

Anahtar kelimeler: Coturnix coturnix, Glikoprotein, Histokimya, Mus musculus, Salmo trutta

Histological and Histochemical Structure of the Some of Digestive Tract Area in Different Vertebrate Species

Summary: In the present observation, histochemical methods were utilized to understand the structural organization and to categorize the diverse classes of glycoproteins eleborated by the epithelium in various regions of digestive tract in different vertebrate species (Mus musculus, Coturnix coturnix and Salmo trutta) In the study, two units adult 4-6 months old quail (Coturnix coturnix) were obtained from Suleyman Demirel University, Department of Animal Science poultry rearing, two trout (Salmo trutta) were provided from breeding pool, two mouse (Mus musculus) were provided from Suleyman Demirel University Experimental Animal Production and Experimental Research Laboratories. In trout, histochemical staining showed a positive reaction in all areas but especially reaction was more powerful in cardia and fundus region. Sulphated mucosubstances were intense in stomach and intestines in quail. In mouse, histochemical apllications were most strong in the lamina epitheliyalis and glands of all region. As a result of histochemical investigations, neutral mucosubstance were more intense than other mucosubstance in trout and mouse, but sulphated mucosubstances were intense in quail.

Key words: Coturnix coturnix, Glycoprotein, Histochemistry, Mus musculus, Salmo trutta.

Keywords

Coturnix coturnix, Glikoprotein, Histokimya, Mus musculus, Salmo trutta

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