Increased DNA Damage of Radiology Personnel Chronically Exposed to Low Levels of Ionizing Radiations

Yıl 2020, Cilt: 11 Sayı: 2, 212 - 216, 15.06.2020

Vehbi Atahan Toğay , Funda Yıldırım Baş , Dilek Aşcı Çelik , Nurten Özçelik , Gülçin Yavuz Türel , Mustafa Calapoğlu , Pinar Aslan Kosar

Öz

Objective:
Occupational life has a direct relation with health. People working in the
hospital units such as Radiology and Nuclear Medicine are subject to higher
doses of ionizing radiation than ordinary person. We examined the association
between DNA damage and ionizing radiation exposure in the personnel working in
university hospital and considered different variables such as smoking, age
etc. Material - Method: DNA Damage
levels were estimated via comet assay in peripheral lymphocytes from 48 exposed
and from 51 non-exposed subjects, aged between 18-57 years old. Tail DNA
percentage parameter, obtained through Open Comet program, was chosen to assess
DNA damage and the results were evaluated by the One - Way Anova statistical test.
Results: The results, obtained from
statistical comparison of tail DNA percentage parameter, indicate that even the
low dose radiation caused DNA damage and age, gender, smoking habits and
working years did not show any significant differences except for dosimetry
value. Increasing dosimetry value resulted in increased DNA damage. Conclusion: This work supports the
previous results of biomonitoring of radiology workers chronically exposed to
ionizing radiation. This means ionizing radiation is still an important DNA
damaging agent despite many improvements such as exposed time reduction,
working conditions and technology.

Anahtar Kelimeler

Ionizing radiation, Comet assay, DNA damage, Radiology personnel

Destekleyen Kurum

Süleyman Demirel Üniversitesi (ÖYP)

Proje Numarası

ÖYP05708-YL-13

Teşekkür

Research permission has been obtained from the Süleyman Demirel University Ethics Committee for Clinical Research (N: 135). We thank to volunteers who participated in this work. This work was supported by Süleyman Demirel University ÖYP Coordination Unit under grant [ÖYP05708-YL-13].

Increased DNA Damage of Radiology Personnel Chronically Exposed to Low Levels of Ionizing Radiations

Öz

Amaç: Çalışma
yaşamının sağlıkla doğrudan ilişkisi bulunmaktadır. Hastanelerin radyoloji veya
nükleer tıp birimlerinde çalışanlar, sıradan insanlara göre daha yüksek
dozlarda iyonize radyasyona maruz kalmaktadır. Çalışmamızda radyoloji
personelinin DNA hasarı ile iyonize radyasyona maruziyetleri arasındaki ilişki
incelenmiş ve sigara içme alışkanlığı ile yaş gibi farklı parametreler de
hesaba katılmıştır. Materyal – Metot:
 18 ila 57 yaş aralığında, 48 kişiden
oluşan radyolojik birimlerde çalışan personel grubu ile 51 kişiden oluşan
kontrol grubunun DNA hasar miktarları komet metodu ile tespit edilmiştir. Open
Comet programı aracılığı ile elde edilen Kuyruk DNA yüzdesi parametresi DNA
hasarını göstermesi için seçilmiş ve sonuçlar tek yönlü anova istatistik testi
ile değerlendirilmiştir. Bulgular: Kuyruk
DNA yüzdesi parametresinin istatistiksel olarak karşılaştırılması sonucunda elde
edilen veriler düşük doz radyasyonun bile DNA hasarına sebep olduğunu ve dozimetre
değerinin önemli bir değişken olduğunu göstermektedir. Dozimetre değeri
arttıkça daha yüksek DNA hasarı tespit edilmiştir. Yaş, cinsiyet, sigara içme
alışkanlığı ve çalışma yılı ise anlamlı bir farklılığa sebep olmamıştır. Sonuç: Sonuçlar, radyoloji personelini
konu alan benzer çalışmalarda bildirilen biyo-izleme verileri ile paralellik
göstermektedir. Bu durum, gelişen teknoloji ve çalışma koşullarının geliştirilmesi
gibi önemli iyileştirmelere rağmen iyonize radyasyonun radyolojik birimlerde
çalışan personel için halen önemli bir DNA hasar ajanı olduğunu ortaya
koymaktadır. 

Anahtar Kelimeler

DNA Hasarı, İyonize Radyasyon, Komet Metodu, Radyoloji Personeli

Proje Numarası

ÖYP05708-YL-13

Kaynakça

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