Işkın (Rheum ribes L.) ekstraktlarının Bakteriyel İletişim Üzerine Etkisi ve Antibakteriyel Aktivitesi

Yıl 2020, Cilt: 11 Sayı: 4, 436 - 442, 31.12.2020

Ebru Önem Hasan Cumhur Sarısu Bashar Ibrahım

Öz

Amaç: Bakteriyel dirençle mücadelede bakteriler arasındaki iletişimin engellenerek virülansın önüne geçilmesi, enfeksiyon hastalıklarıyla mücadelede yeni ve etkili bir seçenek olarak görülmektedir. Birçok bakteri gibi Pseudomonas aeruginosa da bu sistemi alkali proteaz, piyosiyanin, fosfolipaz ve endotoksin gibi virülans faktörlerini sentezlenmesinde, ayrıca enfeksiyon hastalıklarında önemli rol oynayan biyofilm oluşumu ve kayma hareketini yaparken kullanmaktadır. Materyal-Metot: Yapılan bu çalışmada ışkın (Rheum ribes L.) bitkisinin metanol (MeOH), metanol-kloroform (MeOH-CHCl3) ve su (H2O) ekstraktlarının kontrolü çevreyi algılama sistemi tarafından sağlanan P. aeruginosa PA01’in yapmış olduğu kayma hareketi üzerine inhibisyon etkisine bakılmıştır. Ayrıca Gram-pozitif Bacillus cereus ATCC 11778, Enterecoccus faecalis ATCC 29212, Listeria monocytogenes ATCC 7644, Staphylococcus aureus ATCC 25923, Metisilin-Resistant Staphylococcus aureus ATCC 43300 ve Gram-negatif Chromobacterium violaceum ATCC 12472, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Pseudomonas aeruginosa PA01 bakterileri üzerinde antibakteriyel etkileri araştırılarak minimum inhibisyon konsantrasyonları (MIK) belirlenmiştir. Bulgular: Antibakteriyel aktivite sonuçları, tüm ekstraktların çalışmaya dahil edilen Gram-pozitif bakteriler üzerinde farklı oranlarda etkili olup özellikle metanol ekstraktının diğerlerine oranla yüksek aktiviteye (15,3 mm-19,7 mm) sahip olduğunu göstermiştir. Kayma hareketi (swarming motility) üzerine ise metanol ve su ekstraktının inhibisyon etkisi %80 olarak bulunmuş, kloroform ekstraktı ise benzer bir sonuç ile %74 oranında önemli inhibisyon göstermiştir. Sonuç: Işkın bitkisi kök ekstraktlarının özellikle Gram pozitif bakteriler üzerinde antibakteriyel özelliğe sahip olduğu, P. aeruginosa PA01’in yaptığı kayma hareketini inhibe ettiği ve enfeksiyon hastalıkları ile mücadelede yeni nesil ilaçlara model olabilecek bir bitki olma potansiyeline sahip olabileceği düşünülmektedir.

Anahtar Kelimeler

Rheum ribes L. kayma hareketi, çevreyi algılama, PA01, antibakteriyel

The Effect of Rheum ribes L. Extracts on Bacterial Communication and Antibacterial Activity

Öz

Objective: The discovery of quorum sensing systems regulating bacterial virulence has afforded a novel opportunity to control infections without interfering with growth. As many bacteria Pseudomonas aeruginosa also used this system synthesized of some virulence factor such as alkaline protease, pyocyanin, phospholipase and exotoxin A, biofilm formation and swarming motility. Swarming motility is one of the major virulence factors; it is known to play a role in early biofilm development. Material-Method: In this study, inhibitory effect of methanol (MeOH), methanol-chloroform (MeOH-CHCl3) and water (H2O) extracts of the Rheum ribes L. on swarming motility in P. aeruginosa PA01 was investigated. And also antibacterial activity and minimum inhibitory concentration (MIC) values were investigated on Gram-positive Bacillus cereus ATCC 11778, Enterecoccus faecalis ATCC 29212, Listeria monocytogenes ATCC 7644, Staphylococcus aureus ATCC 25923, Metisilin-Resistant Staphylococcus aureus ATCC 43300 and Gram-negative Chromobacterium violaceum ATCC 12472, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Pseudomonas aeruginosa PA01. Results: As a result, all extracts showed antibacterial effect on Gram-positive bacteria in different rate and according to swarming motility experiments, the highest inhibitory effect was seen in methanol and water extracts of Rheum ribes with 81%. Also chloroform extract showed similar inhibition rate (78%) on swarming motility. Conclusion: It is thought that the root extract of the plant has antibacterial properties especially on Gram positive bacteria, inhibits the swarming motility in P. aeruginosa PA01 and may have the potential to be a model for new generation drugs in the fight against infectious diseases.

Anahtar Kelimeler

Rheum ribes L. swarming motility, quorum sensing, PA01, antibacterial

Kaynakça

• 1. Durupınar B. New trends in resistance to antibiotics. Klimik Derg. 2001; (14)2:47-56.
• 2. Tuncer B, Günsan B. Research on regeneration via tissue culture on wild rhubarb (Rheum ribes L.). TURJAF. 2017; 4(3):296-301.
• 3. Meral R. The effect of different temperatures on antioxidant activity and phenolic profile of the Rheum ribes. YYU J Agr Sci. 2017; 27(1):88-94. doi:10.29133/yyutbd.285999.
• 4. Fuqua C, Parsek M, Greenberg E. Regulation of gene expression by cell-to-cell communication: acyl-homoserine lactone quorum sensing. Annual Review of Genetics Department of Biology. 2001; 35:439-468.
• 5. Rutherford ST, Bassler BL. Bacterial Quorum Sensing: Its role in virulence and possibilities for its control. Cold Spring Harb Perspect Med. 2012; 2(11):a012427. doi: 10.1101/cshperspect.a012427.
• 6. Miller MB, Bassler BL. Quorum sensing in bacteria. Annu Rev Microbiol. 2001; 55:165-199.
• 7. Hentzer M, Givskov M. Pharmacological inhibition of quorum sensing for the treatment of chronic bacterial infections. Journal of Clinical Investigation, 2003; 112:1300-1307.
• 8. Lazazzera BA. Grossman AD. The insand outs of peptide signalling. Trends Microbiol. 1998; 6:288-294.
• 9. Hartman G, Wise R. Quorum sensing: potential means of treating gram-negative infections? Lancet. PubMed. 1998; 351:848–849. doi: 10.1016/S0140-6736(05)70282-8.
• 10. Karatuna O, Yağçı A. Virulence factors of Pseudomonas aeruginosa and quorum sensing. Turkish Microbiological Society. 2008; 38(1):42-51.
• 11. Mostafa AA, Al-Askar AA, Almaary KS, Dawoud TM., Sholkamy EN, Bakri MM. Antimicrobial activity of some plant extracts against bacterial strains causing food poisoning diseases. Saudi Journal of Biological Sciences. 2018; 25(2): 361-366
• 12. Dorman HJD, Deans SG. 2000. Antimicrobial agents from plants, antibacterial activity plant volatile oils. Journal of Applied Microbiology, 2000; 88:308-316.
• 13. Lee J, Zhang L. The hierarchy quorum sensing network in Pseudomonas aeruginosa. Protein Cell. 2015; 6(1): 26-41.
• 14. O’Toole GA. Microtiter dish biofilm formation assay. Journal of Visualized Experiments. 2011; 47: 2437.
• 15. Boşgelmez GT. Quorum Sensing in Gram-Negative Bacteria. Turk J Biol. 2003; 27:85-93.
• 16. Murray TS, Ledizet M, Kazmierczak BI. Swarming motility, secretion of type 3 effectors and biofilm formation phenotypes exhibited within a large cohort of Pseudomonas aeruginosa clinical isolates. Journal of Medical Microbiology. 2010; 59: 11–520
• 17. Kearns DB. A field guide to bacterial swarming motility. Nature Reviews Microbiology. 2010; 8(9):634–644.
• 18. Hepdeniz ÖK, Seçkin Ö. Dinamik Mikrobiyal Bir Yaşam: Oral Biyofilm. Suleyman Demirel University The Journal of Health Science. 2017; 8(3): 47-55.
• 19. Nazzaro F, Fratianni F, Martino LD, Coppola R, Feo VD. Effect of essential oils on pathogenic bacteria. Pharmaceuticals. 2013; 6:1451-1474.
• 20. Puupponen-Pimia R, Nohynek L, Meier C, Kähkönen M, Heinonen M, Hopia A, et al. Antimicrobial properties of phenolic compounds from berries. Journal of Applied Microbiology. 2001; 90:494-507.
• 21. Alan Y, Erbil N, Diğrak M. In vivo antimicrobial activity of Rheum ribes extracts obtained from various plant parts from Turkey. Journal of Selçuk University Natural and Applied Science. 2012; 1(4): 23 -29.
• 22. Ceylan S, Çetin S, Camadan Y, Saral O, Ozsen O, Tunus A. Antibacterial and antioxidant activities of traditional medicinal plants from the Erzurum region of Turkey. Irish Journal of Medical Science. 2019; 188(4):1303-1309. doi: 10.1007/s11845-019-01993-x.
• 23. Bazzaz BSF, Khajehkaramadin M, Shokooheizadeh HR. In vitro antibacterial activity of rheum ribes extract obtained from various plant parts against clinical isolates of gram-negative pathogens. Iranian Journal of Pharmaceutical Research. 2005;2: 87-91.
• 24. Alaadin MA, Khateeb EHA, Jäger AK. Antibacterial activity of the iraqi Rheum ribes. Root. Pharmaceutical Biology. 2007; 45(9):688–690. doi:10.1080/13880200701575049.
• 25. Yaşar S. Determination of fixed and essential oil contents and soil characteristic of some perennial medical plants that grow naturally in the campus of Çukurova University [Master thesis]. Adana, Türkiye: Çukurova University; 2005.
• 26. Asımgil A. Şifalı bitkiler. Timaş Yayınları, Aile Sağlığı Dizisi, İstanbul. 1997; 2(176):16-307.
• 27. Ragasa CY, Bacar JNB, Querido MMR, Tan MCS, Oyong GG, Brkljača R, et al. Chemical constituents of Rheum ribes L. International Journal of Pharmacognosy and Phytochemical Research. 2017; 9(1): 65-69.
• 28. Taşkın T, Bulut G. Qualitative and quantitative phytochemical analysis and in-vitro biological activity of Rheum ribes L. different parts. İstanbul Journal of Pharmacy. 2019; 49 (1): 7-13.