sdü tıp fak derg

Medical Journal of Süleyman Demirel University

2602-2109

Süleyman Demirel Üniversitesi

10.17343/sdutfd.1091801

Clinical Sciences

Klinik Tıp Bilimleri

GIDA KATKI MADDESİ SODYUM BENZOATIN OKSİDATİF STRES VE GENOTOKSİSİTE ÜZERİNE ETKİLERİNİN ARAŞTIRILMASI

INVESTIGATION OF THE EFFECTS OF FOOD ADDITIVE SODIUM BENZOATE ON OXIDATIVE STRESS AND GENOTOXICITY

https://orcid.org/0000-0003-3739-9580

İlhan

İlter

SÜLEYMAN DEMİREL ÜNİVERSİTESİ, TIP FAKÜLTESİ

https://orcid.org/0000-0002-3879-9917

Doguc

Duygu

SÜLEYMAN DEMİREL ÜNİVERSİTESİ, TIP FAKÜLTESİ

https://orcid.org/0000-0002-1087-4874

Tepebaşı

Muhammet Yusuf

SÜLEYMAN DEMİREL ÜNİVERSİTESİ, TIP FAKÜLTESİ

https://orcid.org/0000-0001-7935-5004

Sancer

Okan

SÜLEYMAN DEMİREL ÜNİVERSİTESİ

https://orcid.org/0000-0003-0560-042X

Büyükbayram

Halil İbrahim

SÜLEYMAN DEMİREL ÜNİVERSİTESİ, TIP FAKÜLTESİ

09 23 2023

30 3 276 283 03 22 2022 04 13 2022

1994

Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi

AmaçSon yıllarda gıda katkı maddelerinin kullanımında belirginartış vardır. Doğumdan ölüme kadar maruz kalınanbu maddelerin, insanlarda uzun süreli tüketimebağlı olarak oluşturabileceği yan etkiler oldukça önemkazanmıştır. Çalışmamızda özellikle puberte öncesisıçanlarda sodyum benzoata maruziyetin oksidatifstres ve genotoksisite açılarından değerlendirilmesiniamaçladık.Gereç ve YöntemKırk iki adet, 4 haftalık erkek sıçan, kontrol (n=14),deney 1 (Acceptable Daily Intake (ADI) dozunda sodyumbenzoat, n=14) ve deney 2 (No Advers Effect Level(NOAEL) dozunda sodyum benzoat, n=14) gruplarışeklinde 3 gruba ayrılmıştır. Altı hafta süresinceoral gavaj uygulanmıştır. Çalışma sonunda sakrifiyeedilen sıçanların kan örneklerinden biyokimyasal parametreler(glukoz, kreatinin, AST, ALT, ALP, lipid profili,total protein, albümin), total oksidan status (TOS),total antioksidan status (TAS), iskemi modifiye albümin(İMA) ölçülmüş ve Comet yöntemi ile DNA hasarıdeğerlendirilmiştir. Ölçülen TOS ve TAS parametrelerindenoksidatif stres indeksi (OSİ) hesaplanmıştır.BulgularNOAEL dozu sodyum benzoat uygulanan sıçanlardaALT ve kreatinin seviyeleri yükselmiştir. Ayrıca TOS,IMA ve OSİ seviyeleri NOAEL grubunda yüksek ikenTAS seviyeleri düşük bulunmuştur. Bunlara ek olarakNOAEL dozunda sodyum benzoat DNA hasarına yolaçmıştır.SonuçÇalışmamızda, prepubertal dönem sıçanlarda ADIdozunda sodyum benzoat uygulamasının, olumsuzbir etkisi gözlemlenmemiştir. Diğer taraftan NOAELdozunda sodyum benzoat uygulamasının böbrek vekaraciğer fonksiyonlarını bozduğunu ve oksidatif stresile genotoksisiteye yol açabileceği ortaya konmuştur.

ObjectiveRecently, there has been a significant increase in theuse of food additives. Generation of possible sideeffects depending on the long-term consumptionof these substances from birth to death has gainedimportance in humans. In our study, we aimed toevaluate the exposure to sodium benzoate in termsof oxidative stress and genotoxicity, especially inprepubertal rats.Material and MethodIn our study, 42 male rats (4 weeks-old) divided intothree groups as the control (n=14), experiment 1(sodium benzoate Acceptable Daily Intake (ADI) dose,n=14) and experiment 2 (sodium benzoate No AdversEffect Level (NOAEL)dose, n=14) groups and sodiumbenzoate was administration by oral gavage for 6weeks. At the end of the study, rats were sacrified andbiochemical parameters (glucose, creatinine, AST,ALT, ALP, lipid profile, total protein, albumin), totaloxidant status (TOS), total antioxidant status (TAS),ischemia modified albumin (IMA) were measured,and DNA damage was evaluated by Comet analysis.Oxidative stress index (OSI) was calculated from theTOS and TAS parameters.ResultsALT and creatinine levels were increased with theNOAEL dosage of sodium benzoate. In addition, TOS,IMA and OSI levels were elevated, while TAS levelswere decreased in the NOAEL group. Also, NOAELdose of sodium benzoate significantly caused DNAdamage.ConclusionsIn our study, no adverse effect was observed in theadministration of sodium benzoate at the dose ofADI in prepubertal rats. On the other hand, we foundthat NOAEL dose of sodium benzoate impairs kidneyand liver functions and causes oxidative stress andgenotoxicity.

Comet Gıda katkı maddesi Oksidatif stres Sodyum benzoat

Comet Food additive Oxidative stress Sodium benzoate

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