sdü tıp fak derg

Medical Journal of Süleyman Demirel University

2602-2109

Süleyman Demirel Üniversitesi

10.17343/sdutfd.1177877

Clinical Sciences

Klinik Tıp Bilimleri

EFFECT OF ADIPONECTIN ON MCF-7 PROLIFERATION AND GHRELIN LEVEL

ADİPONEKTİN’İN MCF-7 PROLİFERASYONU VE GİRELİN SEVİYESİ ÜZERİNE ETKİSİ

https://orcid.org/0000-0002-5629-3485

Şahin

Uğur

Süleyman Demirel Üniversitesi

https://orcid.org/0000-0001-9207-6931

Şahin

Melda

SÜLEYMAN DEMİREL ÜNİVERSİTESİ

https://orcid.org/0000-0001-7935-5004

Sancer

Okan

SÜLEYMAN DEMİREL ÜNİVERSİTESİ

https://orcid.org/0000-0002-9567-7270

Calapoğlu

Mustafa

SÜLEYMAN DEMİREL ÜNİVERSİTESİ

https://orcid.org/0000-0002-3090-9835

Şenol

Nurgül

SÜLEYMAN DEMİREL ÜNİVERSİTESİ

12 27 2022

29 4 591 595 09 20 2022 10 24 2022

1994

Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi

ObjectiveLocal and systemic factors have been shown to drivethe growth of breast cancer cells in postmenopausalobese women who are at increased risk ofestrogen receptor-positive breast cancer. In obesepostmenopausal women, it has been shown thatcirculating ghrelin hormone is decreased, producedby some tumor cells, and also affects tumor growth.In this study, we aimed to investigate the role ofadiponectin administration in estrogen receptorpositive MCF-7 breast cancer cell line and its effectson ghrelin levels.Material and MethodThe MCF-7 breast cancer cell line was propagatedin 2D culture. The inhibition dose was determined bytreating MCF-7 breast cancer cells with increasingconcentrations of adiponectin (50-5000 ng/mL).Ghrelin levels were measured at the 24th, 48th,and 72nd hours by enzyme-linked immunosorbentassay (ELISA) by administering adiponectin at aconcentration of 50, 100, 250 and 500 ng/mL to theMCF-7 breast cancer cell line.ResultsIn MCF-7 breast cancer cell line culture samples takenat the 24th, 48th and 72nd hours, proliferation wasfound to decrease depending on 50, 100, 250 and500 ng/mL adiponectin concentration. It was foundthat the amount of ghrelin increased depending on 50,100, 250 and 500 ng/mL adiponectin concentration inMCF-7 breast cancer cell line culture samples takenat 24th, 48th and 72nd hours.ConclusionIn our study, we revealed the role of adiponectin,which has proven anti-carcinogenic properties in theMCF-7 breast cancer cell line (ER/PR positive) andalso negatively correlated with obesity, and ghrelin, inER-positive breast cancers.

AmaçLokal ve sistemik faktörlerin, östrojen reseptörü pozitifmeme kanseri riskinde artış olan postmenopozalobez kadınlarda meme kanseri hücrelerinin büyümesiniyönlendirdiği gösterilmiştir. Postmenapozaldönemdeki obez kadınlarda, dolaşımdaki girelin hormonununazaldığı, bazı tümör hücreleri tarafındanüretildiği ve ayrıca tümör büyümesini etkilediği gösterilmiştir.Bu çalışmada, adiponektin uygulamasınınöstrojen reseptörü pozitif MCF-7 meme kanseri hücrehattındaki rolünü ve girelin seviyeleri üzerine olan etkileriniaraştırmayı amaçladık.Gereç ve YöntemMCF-7 meme kanseri hücre hattı 2 boyutlu kültürdeçoğaltıldı. MCF-7 meme kanseri hücrelerine artankonsantrasyonlarda adiponektin (50-5000 ng/mL) ilemuamele edilerek inhibisyon dozu belirlendi. MCF-7meme kanseri hücre hattına 50, 100, 250 ve 500 ng/mL konsantrasyonda adiponektin uygulanarak 24, 48ve 72. Saatlerde örneklerde girelin seviyeleri enzimbağlı immünosorbent analizi (ELISA) ile ölçüldü.Bulgular24, 48 ve 72. saate alınan MCF-7 meme kanseri hücrehattı kültür örneklerinde 50, 100, 250 ve 500 ng/mLadiponektin konsantrasyonuna bağlı olarak proliferasyonunazaldığı bulundu. 24, 48 ve 72. saate alınanMCF-7 meme kanseri hücre hattı kültür örneklerinde50, 100, 250 ve 500 ng/mL adiponektin konsantrasyonunabağlı olarak girelin miktarında artış oluştuğubulundu.SonuçÇalışmamızda MCF-7 meme kanseri hücre hattında(ER/PR pozitif) anti-kanserojenik özelliği kanıtlanmışolan ve aynı zamanda obezite ile negatif korelasyongösteren adiponektinin, girelin ile karşılıklı etkileşimlerinin,ER pozitif meme kanserlerindeki rolünü ortayakoymuş olduk.

MCF-7 Adiponektin Girelin Hücre Kültürü Meme Kanseri

Adiponectin Ghrelin Cell Culture Breast Cancer MCF-7

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