sdü tıp fak derg

Medical Journal of Süleyman Demirel University

2602-2109

Süleyman Demirel Üniversitesi

10.17343/sdutfd.1239914

Clinical Sciences

Klinik Tıp Bilimleri

ANKAFERD BLOOD STOPPER'IN KADMİYUMA BAĞLI GELİŞEN AKUT BÖBREK HASARINA ETKİSİ

PROTECTIVE ROLE OF ANKAFERD BLOOD STOPPER ON CADMIUM-INDUCED ACUTE NEPHROTOXICITY

https://orcid.org/0000-0003-3739-9580

İlhan

İlter

SÜLEYMAN DEMİREL ÜNİVERSİTESİ, TIP FAKÜLTESİ

https://orcid.org/0000-0003-0560-042X

Büyükbayram

Halil İbrahim

SÜLEYMAN DEMİREL ÜNİVERSİTESİ, TIP FAKÜLTESİ

03 14 2023

30 1 111 118 01 20 2023 03 08 2023

1994

Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi

AmaçKadmiyum (Kd) insan sağlığı üzerinde zararlı etkilereneden olabilen çok toksik ve kanserojen bir ağırmetaldir. Kd maruziyetine bağlı olarak özellikle böbreklerdetoksisite gelişebilir. Ankaferd blood stopper(ABS), cerrahide hemostatik özelliği nedeniyle kullanılanbitkisel bir karışımdır. Ayrıca ABS’nin yara vedoku iyileşmesini artırdığı gösterilmiştir. Bu çalışmada,ABS'nin Kd kaynaklı böbrek hasarına karşı olasıkoruyucu etkilerini değerlendirmeyi amaçladık.Gereç ve YöntemOtuz iki erkek sıçan rastgele 4 gruba ayrıldı: kontrol,Kd (kadmiyum klorür, 2,5 mg/kg tek doz ip), ABS(ABS, 1,5 ml/kg tek doz ip) ve Kd+ABS (kadmiyumklorür, 2,5 mg/kg tek doz ip-ABS, 1,5 ml/kg tek doz ip).Deney sonunda sıçanların serumlarından üre ve kreatininseviyeleri ölçüldü. Ayrıca böbrek dokularındanspektrofotometrik olarak total oksidan status (TOS),total antioksidan status (TAS) seviyeleri, süperoksitdismutaz (SOD) ve glutatyon peroksidaz (GPx) enzimaktiviteleri ölçüldü. TOS ve TAS düzeylerinden oksidatifstres indeksi (OSI) hesaplandı. Ayrıca sıçanlarınböbrek dokusunda Bcl-2-associated X protein (Bax),B-cell-lymphoma-2 (Bcl-2), silenced information regulator1 (SIRT1) ve p53'ün mRNA ekspresyonundakideğişiklikler qRT-PCR yöntemi ile değerlendirildi.BulgularKadmiyum grubunda serum üre, kreatinin düzeyleri vedoku oksidatif stres belirteçleri, TOS ve OSI değerlerikontrol grubuna göre anlamlı olarak yüksek (p

ObjectiveCadmium (Cd) is a very toxic and carcinogenic heavymetal that can cause harmful effects on human health.Toxicity may develop due to Cd exposure, especially inthe kidneys. Ankaferd blood stopper (ABS) is a herbalmix that is used for its hemostatic properties in surgery.Also, ABS enhances wound and tissue healing. In thisstudy, we aimed to evaluate the possible ameliorativeeffects of ABS in Cd-induced renal damage.Material and MethodThirty-two male rats were randomly divided into 4groups: control, Cd (cadmium chloride, 2.5 mg/kgsingle dose, ip), ABS (ABS, 1.5 ml/kg single dose ip),and Cd+ABS (cadmium chloride, 2.5 mg/kg single doseip- ABS, 1.5 ml/kg single dose ip). At the end of theexperiment, urea and creatinine levels were analyzedfrom the rats’ serum. In addition, total oxidant status(TOS), total antioxidant status (TAS) levels, superoxidedismutase (SOD), and glutathione peroxidase (GPx)activity were measured spectrophotometricallyfrom renal tissues. The oxidative stress index (OSI)was calculated from TOS and TAS levels. Also, weevaluated alterations in the mRNA expression of Bcl-2-associated X protein (Bax), B-cell-lymphoma-2 (Bcl-2), silenced information regulator 1 (SIRT1), and p53in kidney tissue of rats by using the qRT-PCR method.ResultsIn the Cd group, serum urea, creatinine levels, andtissue oxidative stress markers, TOS and OSI weresignificantly higher while Gpx activity was significantlylower than in the control group (p

Urtica dioica oxidative stress resveratrol licorice thyme

Urtica dioica resveratrol meyan kekik oksidatif stress

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