sdü tıp fak derg

Medical Journal of Süleyman Demirel University

2602-2109

Süleyman Demirel Üniversitesi

10.17343/sdutfd.1268838

Clinical Sciences

Klinik Tıp Bilimleri

THE MMP9 −1562 C/T POLYMORPHISM IS ASSOCIATED WITH INCREASED RISK OF DIABETIC RETINOPATHY IN TURKISH TYPE 2 DIABETES MELLITUS PATIENTS

MMP9 −1562 C/T POLİMORFİZMİ, TÜRK TİP 2 DİABETES MELLİTUS HASTALARINDA ARTMIŞ DİYABETİK RETİNOPATİ RİSKİ İLE İLİŞKİLİDİR

https://orcid.org/0000-0002-4913-9420

Mutlu İçduygu

Fadime

GIRESUN UNİVESITY SCHOOL OF MEDICINE DEPARTMENT OF MEDICAL GENETICS

https://orcid.org/0000-0003-1979-419X

Akgün

Egemen

GIRESUN UNİVESITY SCHOOL OF MEDICINE DEPARTMENT OF MEDICAL BIOLOGY

https://orcid.org/0000-0001-7303-666X

Alp

Ebru

GIRESUN UNİVESITY SCHOOL OF MEDICINE DEPARTMENT OF MEDICAL BIOLOGY

https://orcid.org/0000-0001-6047-218X

Doğuizi

Sibel

UNIVERSITY OF HEALTH SCIENCES, ANKARA ULUCANLAR HEALTH RESEARCH CENTER, DEPARTMENT OF SURGICAL MEDICAL SCIENCES, DEPARTMENT OF OPHTHALMOLOGY

https://orcid.org/0000-0003-1807-6911

Özer

Murat Atabey

GIRESUN UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF SURGICAL MEDICAL SCIENCES, DEPARTMENT OF OPHTHALMOLOGY

06 22 2023

30 2 217 224 03 23 2023 05 29 2023

1994

Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi

ObjectiveType 2 diabetes mellitus (T2DM) and diabeticretinopathy (DR) development is affected by geneticfactors. Matrix metalloproteinases (MMPs) are involvedin many cellular processes, such as remodeling ofextracellular matrix proteins and angiogenesis. Inthis study, we investigated the relationship betweenMMP9 −1562 C/T (rs3918242) polymorphism and thedevelopment of T2DM and DR in Turkish population.Material and MethodA total of 510 participants, involving 168 T2DMcases with DR, 168 T2DM cases without DR,and 174 controls, were included in the study. Thepolymerase chain reaction-restriction fragment lengthpolymorphism (PCR-RFLP) method was carried outto identify genotypes.ResultsThe CT, CT+TT genotypes and the T allele of theMMP9 −1562 C/T polymorphism were associated withincreased risk of DR (p=0.001). On the other hand,no relationship was found between the developmentof T2DM and this polymorphism. In addition,CT (p=0.010) and CT+TT (p=0.015) genotypefrequencies were found to be higher in proliferativediabetic retinopathy (PDR) cases compared to nonproliferativediabetic retinopathy (NPDR) cases, butafter regression analysis, only insulin use (p=0.003)was found to be associated with the development ofPDR.ConclusionOur data show that the MMP9 −1562 C/T polymorphismis associated with the development of DR in TurkishT2DM patients, but no significant relationship wasfound between this polymorphism and the severity ofDR and the development of T2DM.

AmaçTip 2 diabetes mellitus (T2DM) ve diyabetin mikrokomplikasyonlarındanolan diyabetik retinopati (DR)gelişimi genetik faktörlerden etkilenmektedir. Matriksmetalloproteinazlar (MMPs), ekstraselüler matriksproteinlerinin yeniden şekillendirilmesi ve anjiyogenezgibi pek çok hücresel proseste rol alırlar. Bu çalışmadaTürk toplumunda MMP9 −1562 C/T (rs3918242)polimorfizmi ile T2DM ve DR gelişim riski arasındakiilişkinin araştırılması amaçlanmıştır.Gereç ve YöntemMevcut çalışmaya 168 DR’si olan T2DM hastası, 168DR’si olmayan T2DM hastası ve 174 kontrol olmaküzere toplam 510 birey dahil edilmiştir. Genotipleri belirlemekamacıyla Polimeraz zincir reaksiyonu-Restriksiyonfragment uzunluk polimorfizmi (PCR-RFLP)yöntemi kullanılmıştır.BulgularT2DM gelişimi ile MMP9 −1562 C/T polimorfizmi arasındabir ilişkiye rastlanmazken, CT ve CT+TT genotipleriile T (p=0,001) alleli artmış DR riski ile ilişkili bulunmuştur.Ayrıca CT (p=0,010) ve CT+TT (p=0,015)genotip sıklığı proliferatif diyabetik retinopati (PDR)hastalarında proliferatif olmayan diyabetik retinopati(NPDR) hastalarına kıyasla yüksek bulunmuş, fakatregresyon analizi sonrasında sadece insülin kullanımının(p=0,003) PDR gelişimi ile ilişkili olduğu belirlenmiştir.SonuçElde ettiğimiz veriler Türk T2DM hastalarında MMP9−1562 C/T polimorfizminin DR gelişimi ile ilişkili olduğunu,fakat bu polimorfizm ile DR şiddeti ve T2DMgelişimi arasında anlamlı bir ilişki bulunmadığını göstermektedir.

MMP9 Diabetic retinopathy proliferative diabetic retinopathy type 2 diabetes mellitus polymorphism

Diabetik retinopati MMP9 Polimorfizm Proliferatif diyabetik retinopati Tip 2 diabetes mellitus

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