sdü tıp fak derg

Medical Journal of Süleyman Demirel University

2602-2109

Süleyman Demirel Üniversitesi

10.17343/sdutfd.1391007

Gene Expression Transplantation Immunology

Gen İfadesi Nakil İmmünolojisi

Böbrek nakli hastalarında CD8+ T hücrelerindeki PD-1 ve TIM-3 ekspresyon düzeylerinin belirlenmesi

Evaluation of PD-1 And TIM-3 Expression Levels of CD8+ T Cells in Renal Transplant Patients

https://orcid.org/0000-0002-7477-1073

Çerçi Alkaç

Burcu

İZMİR KATİP ÇELEBİ ÜNİVERSİTESİ

https://orcid.org/0000-0001-5159-6463

Soyöz

Mustafa

IZMIR KATIP CELEBI UNIVERSITY

https://orcid.org/0000-0001-8755-4812

Pehlivan

Melek

IZMIR KATIP CELEBI UNIVERSITY

https://orcid.org/0000-0001-7993-978X

Kılıçaslan Ayna

Tülay

IZMIR KATIP CELEBI UNIVERSITY

https://orcid.org/0000-0002-5068-4231

Tatar

Erhan

SAĞLIK BİLİMLERİ ÜNİVERSİTESİ, İZMİR BOZYAKA SAĞLIK UYGULAMA VE ARAŞTIRMA MERKEZİ

https://orcid.org/0000-0002-2886-1166

Tanrısev

Mehmet

UNIVERSITY OF HEALTH SCIENCES, İZMİR TEPECİK HEALTH RESEARCH CENTER

https://orcid.org/0000-0002-3371-7345

Karahan Çöven

Hatice İlayhan

IZMIR KATIP CELEBI UNIVERSITY

https://orcid.org/0000-0001-8485-3286

Pirim

İbrahim

IZMIR KATIP CELEBI UNIVERSITY

06 29 2024

31 2 151 157 11 15 2023 05 10 2024

1994

Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi

Amaç: T hücresi immünoglobulin ve müsin domain 3 (TIM-3) ve programlanmış ölüm 1 (PD-1), T hücre yanıtının düzenlenmesinde önemli roller oynar. Böbrek naklinden sonra CD8+ T hücreleri böbreğe sızarak nekroz, tübülit ve hatta nakil reddine neden olabilir. Sonuç olarak böbrek nakli alıcılarından alınan periferik kan örneklerinde PD-1 ve TIM-3 genlerinin mRNA düzeylerini inceliyoruz.Gereç ve Yöntem: 29 hastadan hem transplantasyon öncesi (tx öncesi) hem de transplantasyon sonrası (tx sonrası) 58 periferik kan örneği toplandı. CD8+ T hücrelerini ayırmak için hemen MACS hücre ayırma sistemi kullanıldı. Toplanan hücrelerin saflığı flow sitometri ile belirlendi. RNA izolasyonu ve cDNA dönüşümünden sonra PD-1 ve TIM-3 genlerinin ifadelerini ölçmek için gerçek zamanlı polimeraz zincir reaksiyonu gerçekleştirildi.Bulgular: Hastaların %31, %45 ve %7'sinde PD-1 seviyelerinde artış, azalma ve değişiklik görülmezken, %76'sında Tim-3 seviyelerinde azalma, %21'inde ise artış görüldü. Ayrıca TIM-3 gen ekspresyon düzeylerinin rejeksiyon ataklarıyla pozitif yönde ilişkili olduğu belirlendi (p

Objective: After kidney transplantation, CD8+ T cells can infiltrate the kidney and cause necrosis, tubulitis, and even transplant rejection. For this reason, control of the T cell response is very important, and T cell immunoglobulin and mucin domain 3 (TIM-3) and programmed death 1 (PD-1) molecules play a role in regulating the T cell response. It is thought that the levels of TIM-3 and PD-1 expressions may be guiding in determining the clinical course after transplantation. This study aimed to determine the relationship between the mRNA levels of PD-1 and TIM-3 genes in peripheral blood samples taken from kidney transplant patients and the clinical conditions of the patients.Material and Method: 60 peripheral blood samples were collected from 30 kidney transplant patients, both pre-transplantation (pre-tx) and post-transplantation (post-tx). CD8+ T cells were separated from other lymphocytes by magnetic cell separation system (MACS) and their purity was determined by flow cytometry. Then, RNA was isolated and after cDNA conversion, the expressions of PD-1 and TIM-3 genes were determined by real-time polymerase chain reaction.Results: While it was determined that the TIM-3 gene expression level increased in patients with acute tubular necrosis, antibody-mediated rejection and cell-mediated rejection findings (p

PD-1 TIM-3 Immunecheckpoint regulators Renal transplantation

PD-1 TIM-3 Bağışıklık kontrol noktası düzenleyicileri Böbrek nakli

Izmir Katip Celebi University Scientific Research Project Coordinator

2019-TDR-SABE-0006

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