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Medical Journal of Süleyman Demirel University

2602-2109

Süleyman Demirel Üniversitesi

10.17343/sdutfd.1648168

Toxicology Histology and Embryology

Toksikoloji Histoloji ve Embriyoloji

The Protective Role of Gilaburu in Amiodarone-Induced Testicular Damage: Immunohistochemical Evaluation via the TNF-α Pathway

https://orcid.org/0000-0003-3190-4486

Özgöçmen

Meltem

SULEYMAN DEMIREL UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF HISTOLOGY AND EMBRYOLOGY

https://orcid.org/0000-0001-8119-078X

Karakeçi

Nazife

NISANTASI UNIVERSITY, NİŞANTAŞI VOCATIONAL SCHOOL, DEPARTMENT OF MEDICAL SERVICES AND TECHNIQUES, PATHOLOGY LABORATORY TECHNIQUES PR. (PAID)

https://orcid.org/0000-0002-9984-294X

Ulusoy Karatopuk

Dilek

SULEYMAN DEMIREL UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF HISTOLOGY AND EMBRYOLOGY

06 19 2025

32 2 169 175 02 27 2025 05 29 2025

1994

Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi

Objective: This study aimed to investigate the protective effects of Gilaburu (GL) in mitigating testicular damage induced by the antiarrhythmic drug Amiodarone (AD) in rats.Materials and Methods: Rats were randomly assigned to four groups: Group Control: received no treatment. Group Amiodarone (AD): received AD (100 mg/kg, intraperitoneally). Group Amiodarone + Gilaburu (GL): received both AD (100 mg/kg, intraperitoneally) and GL (100 mg/kg, orally). Group Gilaburu: received GL alone (100 mg/kg, orally). Following a 10-day experimental period, the animals were euthanized. The left testis was harvested for histological and immunohistochemical examinations, while the right testis and epididymis were collected for testicular weight measurement, sperm isolation, count, and motility analysis.Results: AD administration results in significant testicular damage, as evidenced by histopathological alterations such as irregularly shaped seminiferous tubules, a reduced number of spermatogenic cells, degeneration, and pyknotic nuclei. The highest testicular weight, sperm count, progressive motility, and total motility values were recorded in the control group, whereas the GL group exhibited values comparable to those of the control, with no statistically significant difference observed between them. In contrast, the AD and AD+GL groups demonstrated significant reductions in histopathological analysis, Tumor necrosis factor (TNF-α) stainings, spermiogram analysis (p < 0.05). However, the co-administration of GL with AD mitigated these adverse effects, reducing testicular damage.Conclusion: The findings of this study indicate that Gilaburu has a protective effect against AD-induced testicular damage, potentially attributed to its potent antioxidant properties.

Amiodarone gilaburu testis immunohistochemical analysis TNF- α

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