sdü tıp fak derg

Medical Journal of Süleyman Demirel University

2602-2109

Süleyman Demirel Üniversitesi

10.17343/sdutfd.1682529

Cell Development, Proliferation and Death Cancer Cell Biology Cell Physiology

Hücre Gelişimi, Proliferasyon ve Ölümü Kanser Hücre Biyolojisi Hücre Fizyolojisi

Fig Seed Oil Induces Mitochondrial-Mediated Apoptosis in HepG2 Cells

https://orcid.org/0000-0003-3506-4306

Yavaş

Adem

AYDIN ADNAN MENDERES ÜNİVERSİTESİ, ÇİNE MESLEK YÜKSEKOKULU

09 09 2025

32 3 230 239 04 23 2025 08 21 2025

1994

Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi

Objective: Hepatocellular carcinoma (HCC) remains a significant global health challenge with limited effective therapies, necessitating the identification of novel therapeutic agents. Fig seed oil (FSO), rich in bioactive compounds, is being explored for its health benefits, including potential anti-cancer effects. This study investigated the cytotoxic, pro-apoptotic (via the mitochondrial pathway), and oxidative stress-modulating effects of FSO on HepG2 cells.Material and Method: HepG2 cells were treated with FSO (0.1-1% v/v). Cell viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay over 24, 48, and 72 hours. Oxidative stress markers, including Total Antioxidant Status (TAS), Total Oxidant Status (TOS), and Oxidative Stress Index (OSI), were measured by ELISA. Key apoptotic proteins (Bax, Bcl-2, cytochrome c, cleaved caspase-9, and cleaved caspase-3) were evaluated using Western blot analysis.Results: FSO demonstrated dose-dependent cytotoxicity against HepG2 cells, with an IC50 of 0.45% v/v at 72 hours. Treatment with 0.5% v/v FSO significantly improved cellular oxidative status by increasing TAS (1.8±0.2 vs 1.2±0.1 mmol Trolox eq/L, p

Hepatocellular carcinoma Fig seed oil Apoptosis Mitochondrial pathway Oxidative stress

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