sdü tıp fak derg

Medical Journal of Süleyman Demirel University

2602-2109

Süleyman Demirel Üniversitesi

10.17343/sdutfd.534049

Clinical Sciences

Klinik Tıp Bilimleri

Gundelia tournefortii L. Bitki Ekstraktlarının Antimikrobiyal Aktivitelerinin ve İn Vitro Sitotoksik Aktivitelerinin Değerlendirilmesi

Evaluation of Antimicrobial Activities and In Vitro Cytotoxic Activities of Gundelia tournefortii L. Plant Extracts

Özaltun

Betül

NIGDE OMER HALISDEMIR UNIVERSITY, FACULTY OF MEDICINE Daştan

Taner

CUMHURIYET UNIVERSITY, YILDIZELİ VOCATIONAL SCHOOL, DEPARTMENT OF MEDICAL SERVICES AND TECHNIQUES

12 01 2019

26 4 436 442 03 01 2019 05 03 2019

1994

Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi

Giriş:Kenger, Asteraceae familyasından Gundelia tournefortii bitkisi, tıbbi bir bitkidir. Kenger'in çiçeklerinin, yapraklarının, tohumlarının ve köklerinin besin kaynağı olarak kullanıldığı bilinmektedir.Materyal ve method:Bu çalışmada Sivas ilinde, taze filizleri soyularak çiğ ve pişirilerek yenilen Kenger’in gövde kısımlarının su fazı ve uçucu yağ ekstraksiyonu yapılarak, özütlerin antimikrobiyal ve in vitro antiproliferatif özellikleri bakımından değerlendirilmesi amaçlanmıştır.Sitotoksik aktiviteleriMeme Kanseri Hücre Hattı (MCF-7) ve insan endotelyal hücre hattı(HUVEC)kullanılarak araştırılmıştır. Bitki özütlerinin hücre kültürlerindeki etkileri, XTT yöntemiyle Eliza reader cihazında absorbans bulunarak elde edilmiştir. Kenger bitki özütlerinin MCF-7 hücreleri ve endotelyal hücreler üzerinde sitotoksik etkileri belirlenmiştir.Bulgular:Kenger bitki özütlerinin zayıf antimikrobiyal etkilerinin olduğu MIC değerleriyle ortaya konulmuştur.Sonuç:Sivas ilinde halk arasında şifalı olduğu bilinen, bahar ve yaz aylarında büyüyen bitki kısımları kesilip soyularak iç kısımlarının besin olarak tüketildiği, çeşitli hastalıkların tedavisinde kullanılan Kenger bitkisinin, denediğimiz hücreler üzerinde sitotoksik etkilerinin olduğu, zayıf antimikrobiyal etkisinin bulunduğu görülmektedir.

Introduction:Kenger,Gundelia tournefortiiplant from Asteraceae family, is a medicinal plant. Flowers, leaves, seeds and roots of Kenger have been reported to be used as a source of food.Material ve methods:In this study, it was aimed to evaluate the antimicrobial andin vitroantiproliferative properties of Kenger which fresh sprouts are peeled and eated cooked or uncooked in Sivas. The water phase and essential oil extraction of the body parts of Kenger were aimed to be evaluated in terms of their antimicrobial andin vitroantiproliferative properties.Results:Cytotoxic activities were investigated using the Breast Cancer Cell Line (MCF-7) and human endothelial cell line (HUVEC). The effects of plant extracts on cell cultures were determined by the absorbance of Eliza reader by XTT method. Kenger plant extracts have been shown to have weak antimicrobial effects with MIC values.Conclusions:In the province of Sivas, growing parts of the plant in the spring and summer are cut and peeled into the inner parts of the nutrients. Kenger, which is used in the treatment of various diseases, has cytotoxic effects on the cells we testedand has a weak antimicrobial effect.

Kenger antimicrobial anticarcinogen extraction

Kenger antimikrobiyal antikanserojen ekstraksiyon

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