sdü tıp fak derg

Medical Journal of Süleyman Demirel University

2602-2109

Süleyman Demirel Üniversitesi

10.17343/sdutfd.583950

Clinical Sciences

Klinik Tıp Bilimleri

Elektrik alanın DNA Hasarı ve Beyin Dokusu Üzerine Etkileri - Astaksantin’in Rolü

Aslankoç

Rahime

Kavrık

Oğuzhan

Özmen

Özlem

06 01 2020

27 2 166 172 06 28 2019 07 31 2019

1994

Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi

ÖzetBu çalışmada, uzun süreli elektrik alan(EA) maruziyetinin lenfosit DNA hasarı ve beyin dokusu üzerine etkileriniaraştırmayı amaçladık. Doğal bir karetonoid pigmenti olan astaksantin’in (AST)EA’nın zararlı etkilerini azaltabileceğini düşündük. Çalışmada, 30 adet Wistar Albino (3-4 aylık) dişisıçanlar kullanıldı. Sıçanlar her grupta 10 hayvan olmak üzere rastgele üçgruba ayrıldı; Grup I (Kontrol), Grup II (EA uygulanan grup - 10 kV/m EA, 30gün, günde 23 saat), Grup III (EA+AST tedavi grubu - 10 kV/m EA, 30 gün, günde23 saat + 100 mg/kg/gün AST, 30 gün,gavaj). Sıçanlar deney sonunda sakrifiye edildi. Kan ve beyin dokusubiyokimyasal analizler için toplandı. Kan dokuda comet assay yöntemi ilelenfosit DNA hasarı analizi, beyin dokuda malondialdehit (MDA) seviyesi,süperoksit dismutaz (SOD) ve katalaz (CAT) enzim aktiviteleri çalışıldı. EAuygulanan grupta kontrol grubuna göre MDA ve comet skoru yüksek bulundu. ASTuygulanan grupta EA grubuna göre MDA ve comet skoru azalırken SOD ve CAT enzimaktiviteleri arttı. Çalışma sonuçları EA’ın kan dokuda lenfosit DNA hasarına vebeyin dokuda oksidatif strese neden olduğunu gösterdi. Ayrıca AST tedavisininlenfosit DNA hasarını ve oksidatif stresi azalttığını gösterdi.

Elektrik alan Oksidaitif stres

1. Yokus B, Cakir DU, Akdag MZ, Sert C, Mete N. Oxidative DNA damage in rats exposed to extremely low frequency electro magnetic fields. Free Radical Research 2005; 39(3): 317-323.

2. Paradisi S, Donelli G, Santini MT, Straface E, Malorni W. 50 Hz magnetic field induces structural and biophysical changes in membranes. Bioelectromagnetics 1993; 14(3): 247-255.

3. Kasai H. Analysis of a form of oxidative DNA damage, 8-hydroxy-2′-deoxyguanosine, as a marker of cellular oxidative stress during carcinogenesis. Mutation Research/Reviews in Mutation Research 1997; 387(3): 147-163.

4. Guerin M, Huntly ME, Olaizola M. Haematococcus astaxanthin: applications for human health and nutrition. TRENDS in Biotechnology 2003; 21(5): 210-216.

5. Naguib YMA. Antioxidant activities of astaxanthin and related carotenoids. Journal of agricultural and food chemistry 2000; 48(4):1150-1154.

6. Shimidzu N, Goto M, Miki W. Carotenoids as singlet oxygen quenchers in marine organisms. Fish. Sci. 1996; 62(1):134–137.

7. O’Connor I, O’Brien N. Modulation of UVA light-induced oxidative stress by beta-carotene, lutein and astaxanthin in cultured ﬁbroblasts. J. Dermatol. Sci. 1998; 16(3): 226–230.

8. Tracy RP. Inﬂammation markers and coronary heart disease. Curr. Opin. Lipidol. 1999; 10(5): 435–441.

9. Nakagawa K, Kiko T, Miyazawa T, Burdeos GC. Antioxidant effect of astaxanthin on phospholipid peroxidation in human erythrocytes. British Journal of Nutrition 2011; 105(11): 1563-1571.

10. Engelhart MJ, Geerlings MI, Ruitenberg A, Van Swieten JC, Hofman A, Witteman JCM, Breteler MMB. Intake of vitamin E, vitamin C, and carotenoids and the risk of Parkinson's disease: a meta-analysis. JAMA 2002; 287(24): 3223-3229.

11. Velusamy T, Panneerselvam AS, Purushottam M, Anusuyadevi M, Kumar Pal P, Jain S, Essa MM, Guillemin GJ, Kandasamy M. Protective Effect of Antioxidants on Neuronal Dysfunction and Plasticity in Huntington’s Disease. Oxidative Medicine and Cellular Longevity 2017 (2017): doi.org/10.1155/2017/3279061.

12. Etminan M, Gill SS, Samii A. Intake of vitamin E, vitamin C, and carotenoids and the risk of Parkinson's disease: a meta-analysis. The Lancet Neurology 2005; 4(6): 362-365.

13. Aslankoc R, Gumral N, Saygin M, Senol N, Asci H, Cankara FN, Comlekci S. The impact of electric fields on testis physiopathology, sperm parameters and DNA integrity—The role of resveratrol. Andrologia 2018; 50(4): e12971

14. Drapper HH, Hadley M. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymology 1990; 186: 421–431.

15. Aebi H. Catalase in vitro. Methods Enzymology 1984; 105: 121–126.

16. Woolliams JA, Wiener G, Anderson PH, McMurray CH. Variation in the activities of glutathione peroxidase and superoxide dismutase and in the concentration of copper in the blood various breed crosses of sheep. Research Veterinary Science 1983; 34: 69–77.

17. Collins AR. The comet assay for DNA damage and repair. Molecular Biotechnology 2004; 26: 249–261.

18. Pisoschi AM, Pop A. The role of antioxidants in the chemistry of oxidative stress: A review. European Journal of Medicinal Chemistry 2015; 97: 55-74.

19. Harakawa S, Inoue N, Hori T, Tochio K, Kariya T, Takahashi K, Doge F, Suzuki H, Nagasawa H. Effects of a 50 Hz Electric Field on Plasma Lipid Peroxide Level and Antioxidant Activity in Rats. Bioelectromagnetics 2005;26: 589-594.

20. Mamun Al-Amin Md, Akhter S, Hasan AT, Alam T, Nageeb Hasan SM, Saifullah ARM, Shohel M. The antioxidant effect of astaxanthin is higher in young mice than aged: a region specific study on brain. Metab Brain Dis. 2015; 30:1237–1246.

21. Liu X, Osawa T. Astaxanthin Protects Neuronal Cells against Oxidative Damage and Is a Potent Candidate for Brain Food. Life-style Related Diseases. Yoshikawa T (ed): Food Factors for Health Promotion. Forum Nutr. Basel, Karger, 2009, vol 61, pp 129–135.

22. Chan KC, Mong MC, Yin MC. Antioxidative and Anti‐Inflammatory Neuroprotective Effects of Astaxanthin and Canthaxanthin in Nerve Growth Factor Differentiated PC12 Cells. Journal of Food Science 2009; 74(7): 225-231.

23. Stacey M, Stickley J, Fox P, Statler V, Schoenbach K, Beebe SJ, Buescher S. Differential effects in cells exposed to ultra-short,high intensity electric fields: cell survival,DNA damage, and cell cycle analysis. Mutation Research 2003; 542: 65–75.

24. Schoenbach K, Beebe S, Buescher S. Intracellular effect of ultra-short electric pulses, Bioelectromagnetics 2001; 22:440–448.

25. Azqueta A, Collins AR. Carotenoids and DNA damage. Mutation Research 2012; 733: 4–13.

26. Park JS, Chyun JH, Kim YK, Line LL, Chew BP. Astaxanthin decreased oxidative stress and inflammation and enhanced immune response in humans. Nutrition & Metabolism 2010; 7:18.

27. Tripathi DN, Jena GB. Intervention of astaxanthin against cyclophosphamide-induced oxidative stressand DNA damage: A study in mice. Chemico-Biological Interactions 2009; 180: 398–406.

28. Tripathi DN, Jena GB. Astaxanthin inhibits cytotoxic and genotoxic effects of cyclophosphamidein mice germ cells. Toxicology 2008; 248: 96–103.

29. Santocona M, Zurria M, Berrettini M, Fedeli D, Falcioni G. Influence of astaxanthin, zeaxanthin and lutein on DNA damageand repair in UVA-irradiated cells. Journal of Photochemistry and Photobiology B: Biology 2006; 85: 205–21

30. O'Connor I, O'Brien NM: Modulation of UVA light-induced oxidative stress by β-carotene, lutein and astaxanthin in cultured fibroblasts. J Dermatol Sci. 1998;16: 226-230.