Yıl 2019, Cilt 6 , Sayı 2, Sayfalar 148 - 154 2019-08-01

Tannik asitle irrigasyonun kök kanal dentininin mikrosertliğine ve epoksi rezin esaslı patın bağlanma dayanımına etkisi
Effect of tannic acid irrigation on microhardness of root canal dentin and bond strength of epoxy resin based sealer

Sevinç Askerbeyli Örs [1] , Hacer Aksel [2] , Selen Küçükkaya Eren [3] , Naciye Dilara Zeybek [4]


Amaç: Tannik asit (TA) içeren final irrigasyon protokollerinin dentin mikrosertliğine ve epoksi rezin içerikli patın kök kanal dentinine olan itme bağlanma dayanımına etksinin incelenmesi.

Gereç ve Yöntemler: 50 adet yarım kök mikrosertlik ölçümü için akrilik rezine gömüldü. İşlem öncesi miksrosertlik değerleri elde edildikten sonra, dentin yüzeyleri % 2,5 sodyum hipoklorit (NaOCl) ve %17 etilendiamintetraasetik asit (EDTA) solusyonları ile ikişer dakika muamele edildi. Sonrasında, örnekler kullanılacak olan final irrigasyon protokolüne göre rastgele 5 gruba dağıtıldı (n=10): distile su (kontrol), % 10 TA 2 dk, %10 TA 5dk, % 20 TA 2 dk, ve %20 TA 5 dk. Başlangıç ölçümlerinin yapıldığı noktalara yakın olacak şekilde mikrosertlik değerleri tekrar ölçülüp kaydedildi. İtme bağlanma dayanımı testi için 75 adet tek köklü alt premolar dişin kanalı genişletildi ve irrigasyon protokolü yukarıda bahsedildiği şekilde uygulandı (n = 15). Örneklerin kanalları tek kon yöntemi kullanılarak uyumlu gutaperka konlar ve epoksi rezin içerikli patla doldurulduktan sonra köklerin orta üçlülerinden 1-mm kalınlığında kesitler elde edildi ve itme bağlanma testi uygulandı. Veriler tek yönlü varyans analizi ve Tukey HSD testleri kullanılarak değerlendirildi (p=0,05).

Bulgular: TA uygulanmış gruplarda mikrosertlik değerleri kontrol grubuna göre anlamlı artış gösterirken (p<0,05), TA uygulanmış grupların mikrosertlik değerleri kendi aralarında anlamlı bir fark göstermedi (p>0.05). İtme bağlanma dayanımı testi sonucunda % 10 TA 2 dak. grubu ile kontrol grubu arasında anlamlı fark bulunmadı (p>0,05). Diğer TA grupları kontrol grubuna göre daha yüksek bağlanma dayanımı değerleri gösterirken (p<0,05), aralarında anlamlı fark bulunmadı (p>0,05).

Sonuç:  TA ile final irrigasyon yapılması dentin mikrosertliğini ve epoksi rezin içerikli kanal dolgu patının kök kanal dentinine bağlanma dayanımını arttırdı. TA’nın bağlanma dayanımı üzerindeki etkisi zaman ve konsantrasyon bağımlıdır.

Background: To investigate the effects of final irrigation protocols with tannic acid (TA) on dentin microhardness and push-out bond strength of an epoxy resin based sealer to root canal dentin. 

Materials and Methods: The 50 root halves were embedded in an acrylic resin for the microhardness measurement. After the initial baseline microhardness measurement the root samples were immersed in 2.5% NaOCl and 17% ethylenediaminetetraacetic acid (EDTA) for 2 min, respectively. Then, the root halves were randomly divided into 5 groups according to the final irrigation protocol used (n=10): distilled water (control), 10% TA for 2 min, 10% TA for 5 min, 20% TA for 2 min, and 20% TA for 5 min. After surface treatment, dentin microhardness values were recorded at close proximity to the initial indentation areas. For push-out bond strength test, the root canals of 75 single-rooted mandibular premolars were instrumented and the irrigation protocols were applied as described above (n = 15). Following root canal obturation with single cone technique using matched-taper gutta percha cones and a epoxy resin based root canal sealer, 1 mm-thick slices were obtained from the middle third of the root canals. Push-out bond strength test was applied. Data were analyzed using one way analysis of variance and Tukey HSD tests (p=0.05).

Results: In TA-treated groups, there was a significant increase in the microhardness values compared with the control group (p<0.05) while no significant difference was found among the microhardness values of TA-treated groups (p>0.05). Regarding the push-out bond strength test, there was no significant difference between the bond strength values of 10% TA for 2 min and the control group (p>0.05). The remaining groups presented higher bond strength values than the control group (p<0.05).

Conclusion: TA increased dentin microhardness and the bond strength of the epoxy resin based sealer to root canal dentin. The effect on the bond strength was time and concentration dependent.

  • 1. Schilder H. Filling root canals in three dimensions. Dent Clin North Am 1967; 11: 723-44. 2. Grossman LI. Physical properties of root canal cements. J Endod 1976; 2: 166-75. 3. Ng YL, Mann V, Rahbaran S, Lewsey J, Gulabivala K. Outcome of primary root canal treatment: systematic review of the literature -- Part 2. Influence of clinical factors. J Endod 2008; 41: 6-31. 4. Butler WT. Dentin matrix proteins and dentinogenesis. Connect Tissue Res 1995; 33: 59-65. 5. Al‐Ammar A, Drummond JL, Bedran‐Russo AK. The use of collagen cross‐linking agents to enhance dentin bond strength. J Biomed Mater Res B Appl Biomater. 2009; 91: 419-24. 6. Neelakantan P, Subbarao C, Subbarao CV, De-Deus G, Zehnder M. The impact of root dentine conditioning on sealing ability and push-out bond strength of an epoxy resin root canal sealer. Int Endod J 2011; 44: 491-8. 7. Kalra M, Iqbal K, Nitisusanta LI, Daood U, Sum CP, Fawzy AS. The effect of proanthocyanidins on the bond strength and durability of resin sealer to root dentine. Int Endod J 2013; 46: 169-78. 8. G Pheenithicharoenkul S, Panichuttra A. Epigallocatechin-3-gallate increased the push out bond strength of an epoxy resin sealer to root dentin. Dent Mater J 2016; 35: 888-92 9. Bedran-Russo AK, Yoo KJ, Ema KC, Pashley DH. Mechanical properties of tannic-acid-treated dentin matrix. J Dent Res 2009; 88: 807-11. 10. Zehnder M. Root canal irrigants. J Endod 2006; 32: 389-98. 11. Sayin TC, Serper A, Cehreli ZC, Otlu HG. The effect of EDTA, EGTA, EDTAC, and tetracycline-HCl with and without subsequent NaOCl treatment on the microhardness of root canal dentin. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2007; 104: 418-24. 12. Sim T, Knowles J, Ng YL, Shelton J, Gulabivala K. Effect of sodium hypochlorite on mechanical properties of dentine and tooth surface strain. Int Endod J 2001; 34: 120-32. 13. Moreira DM, Almeida JFA, Ferraz CCR, de Almeida Gomes BPF, Line SRP, Zaia AA. Structural analysis of bovine root dentin after use of different endodontics auxiliary chemical substances. J Endod 2009; 35: 1023-7. 14. Mai S, Kim YK, Arola DD, Gu LS, Kim JR, Pashley DH, et al. Differential aggressiveness of ethylenediamine tetraacetic acid in causing canal wall erosion in the presence of sodium hypochlorite. J Dent 2010; 38: 201-16. 15. Santos JN, de Oliveira Carrilho MR, De Goes MF, Zaia AA, de Almeida Gomes BPF, de Souza-Filho FJ, et al. Effect of chemical irrigants on the bond strength of a self-etching adhesive to pulp chamber dentin. J Endod 2006; 32: 1088-90. 16. Vilanova WV, Carvalho-Junior JR, Alfredo E, Sousa-Neto MD, Silva-Sousa YT. Effect of intracanal irrigants on the bond strength of epoxy resin-based and methacrylate resin-based sealers to root canal walls. Int Endod J 2012; 45: 42-8. 17. Jastrzebska M, Zalewska-Rejdak J, Wrzalik R, Kocot A, Mroz I, Barwinski B, et al. Tannic acid-stabilized pericardium tissue: IR spectroscopy, atomic force microscopy, and dielectric spectroscopy investigations. J Biomed Mater Res A 2006; 78: 148-56. 18. Serrano J, Puupponen-Pimia R, Dauer A, Aura AM, Saura-Calixto F. Tannins: current knowledge of food sources, intake, bioavailability and biological effects. Mol Nutr Food Res 2009; 53: 310-29. 19. Bedran‐Russo AKB, Pereira PN, Duarte WR, Drummond JL, Yamauchi M. Application of crosslinkers to dentin collagen enhances the ultimate tensile strength. J Biomed Mater Res B Appl Biomater 2007; 80: 268-72. 20. Neelakantan P, Sharma S, Shemesh H, Wesselink PR. Influence of Irrigation Sequence on the Adhesion of Root Canal Sealers to Dentin: A Fourier Transform Infrared Spectroscopy and Push-out Bond Strength Analysis. J Endod 2015; 41: 1108-11. 21. Xie Q, Bedran-Russo AK, Wu CD. In vitro remineralization effects of grape seed extract on artificial root caries. J Dent 2008; 36: 900-6. 22. Tang CF, Fang M, Liu RR, Dou Q, Chai ZG, Xiao YH, et al. The role of grape seed extract in the remineralization of demineralized dentine: micromorphological and physical analyses. Arch Oral Biol 2013; 58: 1769-1776. 23. Christopher SR, Mathai V, Nair RS, Angelo JM. The effect of three different antioxidants on the dentinal tubular penetration of Resilon and Real Seal SE on sodium hypochlorite-treated root canal dentin: An in vitro study. J Conserv Dent 2016; 19: 161-5. 24. Ferk Luketic S, Malcic A, Jukic S, Anic I, Segovic S, Kalenic S. Coronal microleakage of two root-end filling materials using a polymicrobial marker. J Endod 2008; 34: 201-3. 25. Balguerie E, van der Sluis L, Vallaeys K, Gurgel-Georgelin M, Diemer F. Sealer penetration and adaptation in the dentinal tubules: a scanning electron microscopic study. J Endod 2011; 37: 1576-9. 26. Sirisha K, Rambabu T, Shankar YR, Ravikumar P. Validity of bond strength tests: A critical review: Part I. J Conserv Dent 2014; 17: 305-11. 27. Pane ES, Palamara JE, Messer HH. Critical evaluation of the push-out test for root canal filling materials. J Endod 2013; 39: 669-73.
Birincil Dil en
Konular Diş Hekimliği
Bölüm Araştırma
Yazarlar

Yazar: Sevinç Askerbeyli Örs

Yazar: Hacer Aksel

Yazar: Selen Küçükkaya Eren

Yazar: Naciye Dilara Zeybek

Tarihler

Yayımlanma Tarihi : 1 Ağustos 2019

Bibtex @araştırma makalesi { selcukdentj598406, journal = {Selcuk Dental Journal}, issn = {}, eissn = {2148-7529}, address = {Selçuk Üniversitesi Diş Hekimliği Fakültesi Alaeddin Keykubat Kampüsü Selçuklu, Konya}, publisher = {Selçuk Üniversitesi}, year = {2019}, volume = {6}, pages = {148 - 154}, doi = {}, title = {Effect of tannic acid irrigation on microhardness of root canal dentin and bond strength of epoxy resin based sealer}, key = {cite}, author = {Askerbeyli Örs, Sevinç and Aksel, Hacer and Küçükkaya Eren, Selen and Zeybek, Naciye Dilara} }
APA Askerbeyli Örs, S , Aksel, H , Küçükkaya Eren, S , Zeybek, N . (2019). Effect of tannic acid irrigation on microhardness of root canal dentin and bond strength of epoxy resin based sealer. Selcuk Dental Journal , 6 (2) , 148-154 . Retrieved from https://dergipark.org.tr/tr/pub/selcukdentj/issue/47415/598406
MLA Askerbeyli Örs, S , Aksel, H , Küçükkaya Eren, S , Zeybek, N . "Effect of tannic acid irrigation on microhardness of root canal dentin and bond strength of epoxy resin based sealer". Selcuk Dental Journal 6 (2019 ): 148-154 <https://dergipark.org.tr/tr/pub/selcukdentj/issue/47415/598406>
Chicago Askerbeyli Örs, S , Aksel, H , Küçükkaya Eren, S , Zeybek, N . "Effect of tannic acid irrigation on microhardness of root canal dentin and bond strength of epoxy resin based sealer". Selcuk Dental Journal 6 (2019 ): 148-154
RIS TY - JOUR T1 - Effect of tannic acid irrigation on microhardness of root canal dentin and bond strength of epoxy resin based sealer AU - Sevinç Askerbeyli Örs , Hacer Aksel , Selen Küçükkaya Eren , Naciye Dilara Zeybek Y1 - 2019 PY - 2019 N1 - DO - T2 - Selcuk Dental Journal JF - Journal JO - JOR SP - 148 EP - 154 VL - 6 IS - 2 SN - -2148-7529 M3 - UR - Y2 - 2019 ER -
EndNote %0 Selcuk Dental Journal Effect of tannic acid irrigation on microhardness of root canal dentin and bond strength of epoxy resin based sealer %A Sevinç Askerbeyli Örs , Hacer Aksel , Selen Küçükkaya Eren , Naciye Dilara Zeybek %T Effect of tannic acid irrigation on microhardness of root canal dentin and bond strength of epoxy resin based sealer %D 2019 %J Selcuk Dental Journal %P -2148-7529 %V 6 %N 2 %R %U
ISNAD Askerbeyli Örs, Sevinç , Aksel, Hacer , Küçükkaya Eren, Selen , Zeybek, Naciye Dilara . "Effect of tannic acid irrigation on microhardness of root canal dentin and bond strength of epoxy resin based sealer". Selcuk Dental Journal 6 / 2 (Ağustos 2019): 148-154 .
AMA Askerbeyli Örs S , Aksel H , Küçükkaya Eren S , Zeybek N . Effect of tannic acid irrigation on microhardness of root canal dentin and bond strength of epoxy resin based sealer. Selcuk Dent J. 2019; 6(2): 148-154.
Vancouver Askerbeyli Örs S , Aksel H , Küçükkaya Eren S , Zeybek N . Effect of tannic acid irrigation on microhardness of root canal dentin and bond strength of epoxy resin based sealer. Selcuk Dental Journal. 2019; 6(2): 154-148.