selcuk j agr food sci Selcuk Journal of Agriculture and Food Sciences 2458-8377 2458-8377 Selçuk Üniversitesi 10.15316/selcukjafsci.1763295 Plant Biotechnology in Agriculture Tarımda Bitki Biyoteknolojisi Aklimatizasyon Aşamasında GA₃ ve AsA Uygulamalarının Vejetatif Gelişim Üzerindeki Etkileri The Effects of GA₃ and AsA Applications on Vegetative Growth During Acclimatization https://orcid.org/0000-0002-1828-7367 Ekinci Heydem Harran Üniversitesi https://orcid.org/0009-0000-3763-9498 Pirinç Ceren https://orcid.org/0000-0003-3828-0522 Şaşkın Necla DİCLE ÜNİVERSİTESİ https://orcid.org/0000-0001-6938-942X Ak Bekir Erol HARRAN ÜNİVERSİTESİ 04 28 2026 40 1 37 51 08 13 2025 12 10 2025 Copyright © 1991, Selcuk Journal of Agriculture and Food Sciences 1991 Selcuk Journal of Agriculture and Food Sciences

In vitro mikroçoğaltım, sağlıklı bitkiciklerin hızlı ve mevsimden bağımsız olarak üretilmesini mümkün kılmakla birlikte, uzun vadeli başarısı büyük ölçüde aklimatizasyon aşamasındaki kayıpların en aza indirilmesine bağlıdır. Kültür kaplarının yüksek nem ve serbest yüzey nemi içermesi nedeniyle bitkicikler yeterli kütikula geliştiremez ve dış koşullara aktarıldıklarında kurumaya karşı son derece duyarlı hâle gelirler. Bu nedenle, aklimatizasyon sırasında destekleyici eksojen uygulamaların yapılması, bitkiciklerin toleransını artırmak ve ölüm oranlarını azaltmak için kritik öneme sahiptir. Bu çalışma, GA₃ (giberellik asit) ve AsA (askorbik asit) uygulamalarının aklimatizasyon sürecinde karşılaşılan kayıpları azaltmadaki ve in vitro koşullarda mikroçoğaltılmış bitkiciklerin adaptasyon başarısını artırmadaki etkilerini değerlendirmek amacıyla yürütülmüştür. Çalışmada yaşama oranı (%), bitki boyu (cm), sürgün çapı (mm), yaprak sayısı (adet/bitkicik), boğum sayısı (adet/bitkicik) ve klorofil içeriği (SPAD) ölçülmüştür.Sonuçlar, en yüksek yaşama oranının (%51,33) 25 ppm GA₃ + 300 mg L⁻¹ AsA uygulamasından elde edildiğini göstermiştir. En uzun sürgünler (18,40 cm) yine 25 ppm GA₃ + 300 mg L⁻¹ AsA uygulamasından elde edilmiştir. En kalın sürgün çapı (1,88 mm) 25 ppm GA₃ + 300 mg L⁻¹ AsA uygulamasında görülmüştür. En yüksek yaprak sayısı (15,00 adet/bitkicik) 25 ppm GA₃ + 150 mg L⁻¹ AsA ve 50 ppm GA₃ + 300 mg L⁻¹ AsA uygulamalarından elde edilmiştir. En yüksek boğum sayısı (14 adet/bitkicik) 50 ppm GA₃ + 300 mg L⁻¹ AsA uygulamasından elde edilmiştir. En yüksek klorofil içeriği (43,49 SPAD) ise 25 ppm GA₃ uygulamasından elde edilmiştir. Bu çalışma, GA₃ ve AsA kombinasyonlarının aklimatizasyon kayıplarını azaltmada etkili olabileceğini ortaya koymaktadır.

In vitro micropropagation enables the rapid and season-independent production of healthy plantlets, yet its long-term success largely depends on minimizing losses during the acclimatization phase. Because the culture vessels contain high humidity and free surface moisture, plantlets develop insufficient cuticles, which makes them highly vulnerable to desiccation when transferred to external conditions. Therefore, implementing supportive exogenous treatments during acclimatization is essential to enhance plantlet tolerance and reduce mortality. This study was conducted to evaluate the effects of gibberellic acid (GA₃) and ascorbic acid (AsA) applications on reducing losses encountered during the acclimatization process and improving the adaptation success of micropropagated plantlets under in vitro conditions. Survival rate (%), plant height (cm), shoot diameter (mm), number of leaves (pieces/plantlet), number of nodes (pieces/plantlet), and chlorophyll content (SPAD) were measured. The results revealed that the highest survival rate (51.33%) was obtained from 25 ppm GA₃ + 300 mg L⁻¹ AsA, the longest shoots (18.40 cm) from 25 ppm GA₃ + 300 mg L⁻¹ AsA, the thickest shoot diameter from 25 ppm GA₃ + 300 mg L⁻¹ AsA (1.88 mm), the highest number of leaves (15.00 pieces/plantlet) from 25 ppm GA₃ + 150 mg L⁻¹ AsA and 50 ppm GA₃ + 300 mg L⁻¹ AsA, the highest number of nodes (14 pieces/plantlet) from 50 ppm GA₃ + 300 mg L⁻¹ AsA, and the highest chlorophyll content (43.49 SPAD) from 25 ppm GA₃. The study demonstrates that combinations of GA₃ and AsA can be effective in reducing acclimatization losses.

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