Bioprospecting Endophytic Fungi For Antiplasmodial Metabolites Through Mechanisti Pathways, Cultivation Challenges And Drug Development Prospect

Oluwole Moses David; Adekemi Olubukunola Oluyege; Francis Jide Faleye; Olugbenga Kayode Popoola; Samuel Oluwadara Borode

doi:10.63673/SJS.1894744

Bioprospecting Endophytic Fungi For Antiplasmodial Metabolites Through Mechanisti Pathways, Cultivation Challenges And Drug Development Prospect

Abstract

Fungal endophytes are becoming recognized as reservoirs of different biologically active secondary metabolites with antimalarial potential. This review focuses on isolation, cultivation, bioactivity, and translational relevance in antimalarial properties of fungal endophyte. Careful plant selection, effective surface sterilization, and appropriate culture conditions remain cardinal for the recovering of endophytic fungi, heretofore challenges in reproducibility and in vitro cultivation remains very difficult. Proper morphological and molecular identification of the fungi provide insights into diversity of the fungal endophyte community and their metabolic bioactivity. In vitro antiplasmodial assays have shown a prospective inhibitory activity of the extracts of endophyte against Plasmodium falciparum with very low IC₅₀ values. Akin to those reported in higher plants, metabolites in endophytes consist of alkaloids, non-ribosomal peptides, polyketides, quinones, terpenoids and xanthones. These phytochemicals have been implicated in heme detoxification, disruption of redox homeostasis, or interruption of key parasite biosynthetic pathways. Promoting in vivo investigations in non-human malaria models aid the bioprospection of these metabolites and further gain insight to toxicity evaluations and mechanism of action. The main problems in bioprospecting of endophyte include adequate sample preparation and identification, also variability in the metabolites, incorrect nomenclature of the isolated chemical candidates. Efforts should be geared toward prioritizing standardization of screening, integration of multi-omics and genome mining and scientific ways of improving the yields of secondary metabolites in discovering potent antimalarial candidates in an era of drug resistance upsurge.

Keywords

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Details

Primary Language

English

Subjects

Analytical Biochemistry, Cellular Interactions

Journal Section

Research Article

Authors

Oluwole Moses David ^*
0000-0002-1396-3450
Nigeria

Adekemi Olubukunola Oluyege This is me
0000-0003-3412-109X
Nigeria

Francis Jide Faleye This is me
Nigeria

Olugbenga Kayode Popoola This is me
0000-0001-6114-7805
Nigeria

Samuel Oluwadara Borode This is me
0000-0003-4929-4786
Nigeria

Publication Date

May 12, 2026

Submission Date

February 21, 2026

Acceptance Date

April 13, 2026

Published in Issue

Year 2026 Volume: 52 Number: 1

DOI

https://doi.org/10.63673/SJS.1894744

IZ

https://izlik.org/JA62CA95DT

Cite

RIS / Bibtex

APA

David, O. M., Oluyege, A. O., Faleye, F. J., Popoola, O. K., & Borode, S. O. (2026). Bioprospecting Endophytic Fungi For Antiplasmodial Metabolites Through Mechanisti Pathways, Cultivation Challenges And Drug Development Prospect. Selcuk Journal of Science, 52(1), 74-102. https://doi.org/10.63673/SJS.1894744

AMA

1.David OM, Oluyege AO, Faleye FJ, Popoola OK, Borode SO. Bioprospecting Endophytic Fungi For Antiplasmodial Metabolites Through Mechanisti Pathways, Cultivation Challenges And Drug Development Prospect. Selcuk J. Sci. 2026;52(1):74-102. doi:10.63673/SJS.1894744

Chicago

David, Oluwole Moses, Adekemi Olubukunola Oluyege, Francis Jide Faleye, Olugbenga Kayode Popoola, and Samuel Oluwadara Borode. 2026. “Bioprospecting Endophytic Fungi For Antiplasmodial Metabolites Through Mechanisti Pathways, Cultivation Challenges And Drug Development Prospect”. Selcuk Journal of Science 52 (1): 74-102. https://doi.org/10.63673/SJS.1894744.

EndNote

David OM, Oluyege AO, Faleye FJ, Popoola OK, Borode SO (May 1, 2026) Bioprospecting Endophytic Fungi For Antiplasmodial Metabolites Through Mechanisti Pathways, Cultivation Challenges And Drug Development Prospect. Selcuk Journal of Science 52 1 74–102.

IEEE

[1]O. M. David, A. O. Oluyege, F. J. Faleye, O. K. Popoola, and S. O. Borode, “Bioprospecting Endophytic Fungi For Antiplasmodial Metabolites Through Mechanisti Pathways, Cultivation Challenges And Drug Development Prospect”, Selcuk J. Sci., vol. 52, no. 1, pp. 74–102, May 2026, doi: 10.63673/SJS.1894744.

ISNAD

David, Oluwole Moses - Oluyege, Adekemi Olubukunola - Faleye, Francis Jide - Popoola, Olugbenga Kayode - Borode, Samuel Oluwadara. “Bioprospecting Endophytic Fungi For Antiplasmodial Metabolites Through Mechanisti Pathways, Cultivation Challenges And Drug Development Prospect”. Selcuk Journal of Science 52/1 (May 1, 2026): 74-102. https://doi.org/10.63673/SJS.1894744.

JAMA

1.David OM, Oluyege AO, Faleye FJ, Popoola OK, Borode SO. Bioprospecting Endophytic Fungi For Antiplasmodial Metabolites Through Mechanisti Pathways, Cultivation Challenges And Drug Development Prospect. Selcuk J. Sci. 2026;52:74–102.

MLA

David, Oluwole Moses, et al. “Bioprospecting Endophytic Fungi For Antiplasmodial Metabolites Through Mechanisti Pathways, Cultivation Challenges And Drug Development Prospect”. Selcuk Journal of Science, vol. 52, no. 1, May 2026, pp. 74-102, doi:10.63673/SJS.1894744.

Vancouver

1.Oluwole Moses David, Adekemi Olubukunola Oluyege, Francis Jide Faleye, Olugbenga Kayode Popoola, Samuel Oluwadara Borode. Bioprospecting Endophytic Fungi For Antiplasmodial Metabolites Through Mechanisti Pathways, Cultivation Challenges And Drug Development Prospect. Selcuk J. Sci. 2026 May 1;52(1):74-102. doi:10.63673/SJS.1894744