Investigation of the cytotoxic effects of juglone on C6 glioma cell line

Ekin Bektas; Mine Kuçak; Firuze Ünlü Bektaş; Tolga Öntürk; Muhammed Hamza Muslumanoglu

Investigation of the cytotoxic effects of juglone on C6 glioma cell line

Abstract

Juglone is an organic compound found naturally in the leaves, roots, bark, and fruits of plants belonging to the Juglandaceae family. It has toxic and growth inhibiting features in many plant species. Glioma is a type of tumorigenesis that occurs when glial cells begin to proliferate un-controllably because of various reasons. C6 glioma cell line is a tumour line derived from brain glial cells of Rattus norvegicus. In this study, after juglone application to C6 glioma cell line, MTT and WST-1 tests were performed and IC50 values were found 6.666 µM and 5.646 µM, respectively. The data obtained showed that the results of two tests were closely related and the cytotoxic effects of juglone were present in this cell line. Furthermore, colony formation assay showed a decrease in colony formation abilities of the cells after juglone treatment. These ob-servations were reinforced by wound healing assay and similarly, the migration ability of the cells decreased with juglone application. Morphological examinations of the cell groups were performed under fluorescent microscopy by DAPI staining, which showed a reduction in cell number. As a final step, the expression levels on the cancer related genes were determined by RT-qPCR. While a significant increase was observed in the expression levels of pro-apoptotic genes compared to the control group, a significant decrease was observed in the expression of the anti-apoptotic gene and the gene involved in proliferation. In line with the data presented, the cytotoxic effects of juglone were determined in the C6 glioma cell line.

Keywords

References

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Details

Primary Language

English

Subjects

Biochemistry and Cell Biology (Other)

Journal Section

Research Article

Authors

Ekin Bektas ^*
0000-0001-5258-2290
Türkiye

Mine Kuçak
0000-0003-3991-4953
Türkiye

Firuze Ünlü Bektaş This is me
0000-0002-0820-9293
Türkiye

Tolga Öntürk
0000-0003-0136-7595
Türkiye

Muhammed Hamza Muslumanoglu
0000-0002-6210-648X
Türkiye

Publication Date

August 1, 2024

Submission Date

October 19, 2022

Acceptance Date

March 12, 2023

Published in Issue

Year 2024 Volume: 42 Number: 4

IZ

https://izlik.org/JA57MJ82JE

Cite

RIS / Bibtex

APA

Bektas, E., Kuçak, M., Ünlü Bektaş, F., Öntürk, T., & Muslumanoglu, M. H. (2024). Investigation of the cytotoxic effects of juglone on C6 glioma cell line. Sigma Journal of Engineering and Natural Sciences, 42(4), 965-972. https://izlik.org/JA57MJ82JE

AMA

1.Bektas E, Kuçak M, Ünlü Bektaş F, Öntürk T, Muslumanoglu MH. Investigation of the cytotoxic effects of juglone on C6 glioma cell line. SIGMA. 2024;42(4):965-972. https://izlik.org/JA57MJ82JE

Chicago

Bektas, Ekin, Mine Kuçak, Firuze Ünlü Bektaş, Tolga Öntürk, and Muhammed Hamza Muslumanoglu. 2024. “Investigation of the Cytotoxic Effects of Juglone on C6 Glioma Cell Line”. Sigma Journal of Engineering and Natural Sciences 42 (4): 965-72. https://izlik.org/JA57MJ82JE.

EndNote

Bektas E, Kuçak M, Ünlü Bektaş F, Öntürk T, Muslumanoglu MH (August 1, 2024) Investigation of the cytotoxic effects of juglone on C6 glioma cell line. Sigma Journal of Engineering and Natural Sciences 42 4 965–972.

IEEE

[1]E. Bektas, M. Kuçak, F. Ünlü Bektaş, T. Öntürk, and M. H. Muslumanoglu, “Investigation of the cytotoxic effects of juglone on C6 glioma cell line”, SIGMA, vol. 42, no. 4, pp. 965–972, Aug. 2024, [Online]. Available: https://izlik.org/JA57MJ82JE

ISNAD

Bektas, Ekin - Kuçak, Mine - Ünlü Bektaş, Firuze - Öntürk, Tolga - Muslumanoglu, Muhammed Hamza. “Investigation of the Cytotoxic Effects of Juglone on C6 Glioma Cell Line”. Sigma Journal of Engineering and Natural Sciences 42/4 (August 1, 2024): 965-972. https://izlik.org/JA57MJ82JE.

JAMA

1.Bektas E, Kuçak M, Ünlü Bektaş F, Öntürk T, Muslumanoglu MH. Investigation of the cytotoxic effects of juglone on C6 glioma cell line. SIGMA. 2024;42:965–972.

MLA

Bektas, Ekin, et al. “Investigation of the Cytotoxic Effects of Juglone on C6 Glioma Cell Line”. Sigma Journal of Engineering and Natural Sciences, vol. 42, no. 4, Aug. 2024, pp. 965-72, https://izlik.org/JA57MJ82JE.

Vancouver

1.Ekin Bektas, Mine Kuçak, Firuze Ünlü Bektaş, Tolga Öntürk, Muhammed Hamza Muslumanoglu. Investigation of the cytotoxic effects of juglone on C6 glioma cell line. SIGMA [Internet]. 2024 Aug. 1;42(4):965-72. Available from: https://izlik.org/JA57MJ82JE