ANTI-OXIDATIVE EFFECTS OF GYPSOPHILA PERFOLIATA ON D-GALACTOSE INDUCED AGING IN RAT

Yıl 2020, Cilt: 38 Sayı: 4, 1657 - 1664, 05.10.2021

Korkut Furkan Şahin Emel Serdaroğlu Kaşıkçı Burcu Çevreli Gökhan Zengin Tayfun Gözler Muhsin Konuk

Öz

D-Galactose (D-GAL), a monosaccharide, induces the oxidative stress and results in the mimicked aging model in rats. Gypsophila perfoliata belongs to Caryophyllaceae family and a perennial plant. The content of this plant is rich in saponarin and Phenolics, therefore scientist has been focused on its possible antioxidative effects. In this study, we studied the antioxidant effect of Gypsophila perfoliata on aging mimicked rats induced by D-GAL. For this, Wistar-albino rats (n=8 in each group of 4) have been applied D-GAL (150 mg/kg/day) subcutaneously (s.c.) and Gypsophila extract (100 mg/kg/day) via gavage for 12 weeks. After this period, the rats were sacrificed and their blood serum and brains used to determine Glutathione (GSH) and Malondialdehyde (MDA). The data were analyzed by use of the SPSS software package (20th version) One-way ANOVAs followed by post-hoc Bonferroni tests were used, and p< 0.05 was considered as significantly different value. When the data of examined groups compared to the control, we observed a significant increase in the MDA while significant decrease in the GSH levels. However, when we compared the Gypsophila group with D-GAL, the levels of both the GSH and MDA varied significantly. Our findings showed that the extract of Gypsophila could be used in reducing the oxidative stress during the aging.

Anahtar Kelimeler

Aging, D-galactose, Gypsophila perfoliata, lipid peroxidation.

Kaynakça

• Sullivan J, Mirbahai L, & Lord J M., (2018) Major trauma and acceleration of the ageing process. Ageing Res Rev.
• ⦁ Ristow M, Schmeisser S., (2011) Extending life span by increasing oxidative stress. Free Radic Biol Med, 327-336.
• ⦁ Serrano F, Klann E., (2004) Reactive oxygen species and synaptic plasticity in the aging hippocampus. Ageing Res Rev,431–443.
• ⦁ Schieber M, Chandel NS., (2014) ROS function in redox signaling and oxidative stress. Current biology, R453-R462.
• ⦁ Pandey KB, Rizvi SI., (2009) Plant polyphenols as dietary antioxidants in human health and disease. Oxid Med Cell Longev,270-278.
• ⦁ Simeonova R, Vitcheva V, Krasteva I, Zdraveva P, Konstantinov S, Ionkova I., (2016) Antidiabetic and antioxidant effects of saponarin from Gypsophila trichotoma on streptozotocin-induced diabetic normotensive and hypertensive rats. Phytomedicine, 483-490.
• ⦁ Çoban J, Doğan-Ekici I, Aydın AF, Betül-Kalaz E, Doğru-Abbasoğlu S, Uysal M., (2015) Blueberry treatment decreased D-galactose-induced oxidative stress and brain damage in rats. Metab Brain Dis,30(3), 793-802.
• ⦁ Song EK, Park H, & Kim HS., (2018) Additive effect of walnut and chokeberry on regulation of antioxidant enzyme gene expression and attenuation of lipid peroxidation in d-galactose-induced aging-mouse model. Nutrition Research.
• ⦁ Kasikci ES, Erguzel TT, Sayyed MI, Karahan M, Altunsoy EE, Singh VP, ... Konuk M., (2018) Gamma Shielding Properties of Erbium Zinc Tellurite Glass System Using Monte Carlo Method. Journal of Testing and Evaluation, 48(2).
• ⦁ Aydın AF, Çoban J, Doğan-Ekici I, Betül-Kalaz E, Doğru-Abbasoğlu S, Uysal M., (2016) Carnosine and taurine treatments diminished brain oxidative stress and apoptosis in D-galactose aging model. Metab Brain Dis,31(2), 337-345.
• ⦁ Böttger S, Melzig MF., (2011) Triterpenoid saponins of the Caryophyllaceae and Illecebraceae family. Phytochem Lett,4(2), 59-68.
• ⦁ Korkmaz M; Özçelik H., (2011) Economic importance of Gypsophila L., Ankyropetalum fenzl and Saponaria L.(Caryophyllaceae) taxa of Turkey. African journal of Biotechnology,10(47), 9533-9541.
• ⦁ Baytop T., ,(1984) Treatment with plants in Turkey. Istanbul Univ. Publ, 3255.
• ⦁ Vitcheva V, Simeonova R, Krasteva I, Yotova M, Nikolov S, Mitcheva M., (2011) Hepatoprotective effects of saponarin, isolated from Gypsophila trichotoma Wend. on cocaine-induced oxidative stress in rats. Redox Rep,16(2), 56-61.
• ⦁ Huang QF, Zhang SJ, Zheng L, Liao M, He M, Huang R, ... Lin X., (2012) Protective effect of isoorientin-2 ″-O-α-l-arabinopyranosyl isolated from Gypsophila elegans on alcohol induced hepatic fibrosis in rats. Food Chem Toxicol,50(6), 1992-2001.
• ⦁ Zheleva-Dimitrova D, Zengin G, Balabanova V, Voynikov Y, Lozanov V, Lazarova I, Gevrenova R., (2018) Chemical characterization with in vitro biological activities of Gypsophila species. J Pharm Biomed Anal,155, 56-69.
• ⦁ Liguori, I., Russo, G., Curcio, F., Bulli, G., Aran, L., Della-Morte, D., ... & Abete, P., (2018) Oxidative stress, aging, and diseases. Clinical interventions in aging, 13, 757.
• ⦁ Samarghandian, S., Azimi-Nezhad, M., & Farkhondeh, T., (2016) Preventive effect of carvacrol against oxidative damage in aged rat liver. Int. J. Vitam. Nutr. Res, 1-8.
• ⦁ Beutler E., (1963) Improved method for the determination of blood glutathione. J. lab. clin. Med.,61, 882-888.
• ⦁ Ohkawa H, Ohishi N, Yagi K., (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical biochemistry,95(2), 351-358.
• ⦁ Rodríguez-Arellano JJ, Parpura V, Zorec R, Verkhratsky A., (2016) Astrocytes in physiological aging and Alzheimer’s disease. Neuroscience,323, 170-182.
• ⦁ Moura LFWG, da Silva Neto JX, Lopes TDP, Benjamin SR, Brito FCR, Magalhães FEA, ... Guedes MIF., (2019) Ethnobotanic, phytochemical uses and ethnopharmacological profile of genus Cnidoscolus spp.(Euphorbiaceae): A comprehensive overview. Biomed Pharmacother, 109, 1670-1679.
• ⦁ Serdaroglu-Kasikci E., (2018) Evaluation of longterm quercetin administration on age related oxidative stress induced by D-Galactose in rats. Fresenius Environ Bull,27(11), 7781-7786.
• ⦁ Kaşıkçı ES, Gökalp HK., (2018) Sıçanlarda D-Galaktoz ile Uyarılmış Yaşlanma Modelinde Kefir’in Beyin Dokusundaki Oksidatif Stres Üzerine Etkisi. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi,22, 221-228.
• ⦁ Kamiyama M, Shibamoto, T., (2012) Flavonoids with potent antioxidant activity found in young green barley leaves. Journal of agricultural and food chemistry,60(25), 6260-6267.
• ⦁ Simeonova R, Vitcheva V, Kondeva-Burdina M, Krasteva I, Manov V, Mitcheva M., (2013) Hepatoprotective and antioxidant effects of saponarin, isolated from Gypsophila trichotoma Wend. on paracetamol-induced liver damage in rats. BioMed research international.
• ⦁ Wu QX, Li Y, Shi YP., (2006) Antioxidant phenolic glucosides from Gentiana piasezkii. J Asian Nat Prod Res,8(5), 391-396.
• ⦁ Ielciu I, Frédérich M, Hanganu D, Angenot L, Olah NK, Ledoux A, ... Păltinean R., (2019) Flavonoid Analysis and Antioxidant Activities of the Bryonia alba L. Aerial Parts. Antioxidants,8(4), 108.
• ⦁ Sengupta S, Mukherjee A, Goswami R, Basu, S., (2009) Hypoglycemic activity of the antioxidant saponarin, characterized as α-glucosidase inhibitor present in Tinospora cordifolia. J Enzyme Inhib Med Chem,24(3), 684-690.
• ⦁ Shwe T, Pratchayasakul W, Chattipakorn N, Chattipakorn SC., (2018) Role of D-galactose-induced brain aging and its potential used for therapeutic interventions. Experimental gerontology,101, 13-36.
• ⦁ Bai F, Huang Q, Wei J, Lv S, Chen Y, Liang C, Wei L, Lu Z, Lin X., (2018) Gypsophila elegans isoorientin-2-O-α-l-arabinopyranosyl ameliorates porcine serum-induced immune liver fibrosis by inhibiting NF-KB signalling pathway and suppressing HSC activation. Int Immunopharmacology, 54:60-67.