Effect of diflubenzuron on the development of chick embryo

Abstract

Aim: To check the effect of drug Difenoconazole on chick embryo. Difenoconazole which is a fungicide sprayed on plants and cause toxic effect on non -targeted species. Few studies have investigated that body weight of zebra fish reduced by difenoconazole. Our aim of study to see the effect of Difenoconazole on chick embryo growth. Methodology: A total 56 eggs were selected and categorized into four groups (one control and other three were treated groups). At fifth day of incubation were treated with drug and first sacrificed done on eleventh day of incubation for examination. Three eggs from each group were sacrificed. The second sacrificed done on nineteen day of incubation three eggs from each group were sacrificed and examined their growth. Major findings: Higher growth was observed in the control group. In treatment 1, heart formation and angiogenesis was occurred. In treatment 2, mostly eggs aborted and some showed little growth. While results of treatment 3 showed very little development of chick embryo. Thus, higher doses of difenoconazole proved more toxic and lethal. Implications of the study: This study demonstrates that difenoconazole has a significant teratogenic potential on chick embryo because it caused abortion and inhibits the growth and development of chick embryo, thus its use should be limited.

Keywords

• Chick embryo,Growth,,Difenoconazole,Toxic

References

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