Mespilus Germanica (L)'nın Antioksidan, Antimikrobiyal Aktivitesi ve DNA Koruyucu Etkisi

Abstract

Amaç: Muşmula (M. germanica ) sert meyveleri olan dayanıklı bir bitkidir. Bu çalışmada muşmulanın hem meyve hem de tokum ekstraktının antioksidan, antimikrobiyal aktivitesi ve DNA koruyucu etkilerinin belirlenmesi amaçlanmıştır. Yöntem: Muşmula ekstraktlarının antioksidan kapasitesi DPPH yöntemi kullanılarak belirlenmiş, toplam fenolik içerik için Folin-Ciocaltaeu yöntemi kullanılmış ve ayrıca ekstraktın 16 bakteriye karşı antimikrobiyal etkisi araştırılmıştır. Bulgular: Çalışma sonucunda farklı konsantrasyonlarda elde edilen muşmula meyve ekstraktların DNA hasarına karşı olası koruyucu etkisi belirlendi. Bunun yanı sıra tohum ekstraktlarının toplam fenolik içeriklerinin ve radikal yakalama aktivitelerinin meyve ekstraktından daha yüksek olduğu ortaya çıktı. Çalışmada kullanılan muşmula tohum ekstraktını bakterilere karşı herhangi bir etki göstermezken,bu bitkinin meyve ekstraktının kullanılan bakteriler üzerinde çeşitli etkileri oldu.Hem UV'nin hemde H2O2’nin muşmula DNA'sına zarar verdiği ancak her iki stresin birlikte uygulamasının daha zararlı olduğu belirlendi. Sonuç: Elde edilen sonuçlara göre, M. germanica hücrede oksidatif strese karşı koruma sağlayabildiği gözlendi.

Keywords

• Antimikrobiyal;
• ; DNA koruyucu etki
• Serbest radikal süpürücü
• Mespilus germanica
• Fenolik içerik

ANTIOXIDANT, ANTIMICROBIAL ACTIVITY AND DNA PROTECTIVE EFFECT OF MESPILUS GERMANICA (L)

Abstract

Purpose: Medlar (M. germanica ) is a durable plants which have hard fruits. In this study, it was aimed to determine the antioxidant, antimicrobial activity and DNA protective effects of both fruit and seed extracts of medlar. Methods: Antioxidant capacities of extracts of medlar was determined by using the DPPH method, Folin-Ciocaltaeu method was used for total phenolic content and also the antimicrobial effect of the extract against 16 bacteria was investigated. Results: As a result of the study, the possible protective effect of medlar fruit extracts obtained at different concentrations against DNA damage was determined. In addition, it was revealed that the total phenolic contents and radical scavenging activities of the seed extracts were higher than the fruits extracts. While the medlar seed extract used in the study did not show any effect against bacteria, the fruit extract of this plant had various effects on the bacteria used. It was determined that both UV and H2O2 damaged the medlar DNA, but the application of both stresses together was more harmful. Conclusion: According to the results obtained, it was observed that M. germanica could provide protection against oxidative stress in the cell.

Keywords

• Antimicrobial
• DNA protective effect
• Free radical scavenging
• Mespilus germanica
• Phenolic contents

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