Volatile components of Stachys cretica subsp. anatolica Rech. F., Ann. in Koçtepe province of Isparta, Turkey

Turkey is an important gene center in terms of the Lamiaceae family plants, which take the first place among the plant species traded on the world market and have an important role in alternative medicine applications. Stachys is one of the important genus of Lamiaceae and represented by approximately 200 taxa. In this study which was conducted in 2017 and 2018, above ground parts of Stachys cretica subsp. anatolica in flowering period of plant were collected from Koçtepe province of Isparta. Volatile components were determined by gas chromatography mass spectroscopy (GC-MS) after solid phase micro extraction (SPME). Collected samples were placed in paper packages and transported to the laboratory on the same day and dried at room temperature (25°C), after they were subjected to solid phase microextraction (SPME). 2 g of samples were placed into a 10 mL vial. After incubation for 30 min at 60°C, SPME fibre was pushed through the headspace of a sample vial to adsorbed the volatiles, and then inserted directly into the injection port of the GC-MS (Shimadzu 2010 Plus) at a temperature of 250°C for desorption (5 min) of the adsorbed volatile compounds for analysis. As result, 58 volatile components were determined and (E)-2-hexenal (12.58%), germacrene-D (34.56%) and also β-caryophyllene (21.04%) were found as main components. Stachys species are becoming increasingly important due to the aromatic compounds and essential oils they contain, as well as being the plants that are used by the public in the treatment of diseases and which are medicinal properties.