Yıl 2000, Cilt 24 , Sayı 2, Sayfalar 177 - 188 2000-02-01

Morphological Changes in Xenopus laevisEmbryos with Lithium and Evaluation of the Fetax Test
Morphological Changes in Xenopus laevisEmbryos with Lithium and Evaluation of the Fetax Test

Ayper BOĞA [1] , Seçil BİNOKAY [2] , Ayşe KENDİRCİ [3] , Tuncay ÖZGÜNEN [4]


The main goal was to standardize the FETAX (Frog Embryos Teratogenesis Assay: Xenopus) test. Since lithium ions are known to be teratogenic for embryos of many organisms, they were prefferred for our first standardization assay. Xenopus laevis embryos were exposed to lithium chloride (from 2x10 -4 to 6 x 10 -3 M concentrations) for 96 hours at 2-4 cell stages of development. Exposure to lower LiCl concentrations permitted larvae to survive and to develop into normal ones. On the other hand, depending on the increase in the LiCl concentration, the body length and survival rate (%) decreased. In addition, the decreased survival and increased malformation rates were shown to be parallel with the increased concentration rates. The most common abnormalities were found to be tail kinks and edema. The less frequents abnormalities were microcephaly, cyclopia, shortening of the trunk (micromyelia), depigmentation, macrocephaly, spina bifida, anteriozation, posteriozation, medially located eyes and abnormally of the yolk. In the Fetax test, at ninety-six hours, LC 50 value was 0.002 g/L, and EC 50 value was 0.0088g/L for lithium chloride. Finally, the teratogenicity index was found to be about 2.5. These results showed that lithium chloride was a moderate teratogen.
The main goal was to standardize the FETAX (Frog Embryos Teratogenesis Assay: Xenopus) test. Since lithium ions are known to be teratogenic for embryos of many organisms, they were prefferred for our first standardization assay. Xenopus laevis embryos were exposed to lithium chloride (from 2x10 -4 to 6 x 10 -3 M concentrations) for 96 hours at 2-4 cell stages of development. Exposure to lower LiCl concentrations permitted larvae to survive and to develop into normal ones. On the other hand, depending on the increase in the LiCl concentration, the body length and survival rate (%) decreased. In addition, the decreased survival and increased malformation rates were shown to be parallel with the increased concentration rates. The most common abnormalities were found to be tail kinks and edema. The less frequents abnormalities were microcephaly, cyclopia, shortening of the trunk (micromyelia), depigmentation, macrocephaly, spina bifida, anteriozation, posteriozation, medially located eyes and abnormally of the yolk. In the Fetax test, at ninety-six hours, LC 50 value was 0.002 g/L, and EC 50 value was 0.0088g/L for lithium chloride. Finally, the teratogenicity index was found to be about 2.5. These results showed that lithium chloride was a moderate teratogen.
Birincil Dil tr
Bölüm Makaleler
Yazarlar

Yazar: Ayper BOĞA

Yazar: Seçil BİNOKAY

Yazar: Ayşe KENDİRCİ

Yazar: Tuncay ÖZGÜNEN

Tarihler

Yayımlanma Tarihi : 1 Şubat 2000

Bibtex @ { tbtkbiology140234, journal = {Turkish Journal of Biology}, issn = {1300-0152}, eissn = {1303-6092}, address = {}, publisher = {TÜBİTAK}, year = {2000}, volume = {24}, pages = {177 - 188}, doi = {}, title = {Morphological Changes in Xenopus laevisEmbryos with Lithium and Evaluation of the Fetax Test}, key = {cite}, author = {BOĞA, Ayper and BİNOKAY, Seçil and KENDİRCİ, Ayşe and ÖZGÜNEN, Tuncay} }
APA BOĞA, A , BİNOKAY, S , KENDİRCİ, A , ÖZGÜNEN, T . (2000). Morphological Changes in Xenopus laevisEmbryos with Lithium and Evaluation of the Fetax Test. Turkish Journal of Biology , 24 (2) , 177-188 . Retrieved from https://dergipark.org.tr/tr/pub/tbtkbiology/issue/11748/140234
MLA BOĞA, A , BİNOKAY, S , KENDİRCİ, A , ÖZGÜNEN, T . "Morphological Changes in Xenopus laevisEmbryos with Lithium and Evaluation of the Fetax Test". Turkish Journal of Biology 24 (2000 ): 177-188 <https://dergipark.org.tr/tr/pub/tbtkbiology/issue/11748/140234>
Chicago BOĞA, A , BİNOKAY, S , KENDİRCİ, A , ÖZGÜNEN, T . "Morphological Changes in Xenopus laevisEmbryos with Lithium and Evaluation of the Fetax Test". Turkish Journal of Biology 24 (2000 ): 177-188
RIS TY - JOUR T1 - Morphological Changes in Xenopus laevisEmbryos with Lithium and Evaluation of the Fetax Test AU - Ayper BOĞA , Seçil BİNOKAY , Ayşe KENDİRCİ , Tuncay ÖZGÜNEN Y1 - 2000 PY - 2000 N1 - DO - T2 - Turkish Journal of Biology JF - Journal JO - JOR SP - 177 EP - 188 VL - 24 IS - 2 SN - 1300-0152-1303-6092 M3 - UR - Y2 - 2020 ER -
EndNote %0 Turkish Journal of Biology Morphological Changes in Xenopus laevisEmbryos with Lithium and Evaluation of the Fetax Test %A Ayper BOĞA , Seçil BİNOKAY , Ayşe KENDİRCİ , Tuncay ÖZGÜNEN %T Morphological Changes in Xenopus laevisEmbryos with Lithium and Evaluation of the Fetax Test %D 2000 %J Turkish Journal of Biology %P 1300-0152-1303-6092 %V 24 %N 2 %R %U
ISNAD BOĞA, Ayper , BİNOKAY, Seçil , KENDİRCİ, Ayşe , ÖZGÜNEN, Tuncay . "Morphological Changes in Xenopus laevisEmbryos with Lithium and Evaluation of the Fetax Test". Turkish Journal of Biology 24 / 2 (Şubat 2000): 177-188 .
AMA BOĞA A , BİNOKAY S , KENDİRCİ A , ÖZGÜNEN T . Morphological Changes in Xenopus laevisEmbryos with Lithium and Evaluation of the Fetax Test. Turkish Journal of Biology. 2000; 24(2): 177-188.
Vancouver BOĞA A , BİNOKAY S , KENDİRCİ A , ÖZGÜNEN T . Morphological Changes in Xenopus laevisEmbryos with Lithium and Evaluation of the Fetax Test. Turkish Journal of Biology. 2000; 24(2): 188-177.