Yıl 1999, Cilt 23 , Sayı 4, Sayfalar 393 - 400 1999-04-01

Production of Monoclonal Antibodies specific for Progesterone
Production of Monoclonal Antibodies specific for Progesterone

Fatıma YÜCEL [1]


Progesterone levels in milk and serum are indicators of pregnancy in cattle. The progesterone level reaches a peak on the 21 st and 22 nd days of pregnancy. Monoclonal antibodies specific to progesterone could be used for the immunodetection of milk and serum progesterone levels. We report here the development of hybrid cells prdoducing monoclonal antibodies specific for progesterone using hybridoma technology. Hybridoma cells secreting monoclonal antibodies against progesterone (MAM 2H11, MAM 6G6) were developed by fusion of the spleen cells of an immunized BALB/c mouse with FO (ATCC CRL 1646) mouse myeloma cells. These monoclonal antibodies were characterized for use in the development of diagnostic kits based on the enzyme-linked immunosorbent assay (ELISA) test system. ELISA tests showed that antibodies produced by 2 hybrids from 375 wells reacted specifically with progesterone. Monoclonal antibodies did not show any cross reaction with other steroid hormones.
Progesterone levels in milk and serum are indicators of pregnancy in cattle. The progesterone level reaches a peak on the 21 st and 22 nd days of pregnancy. Monoclonal antibodies specific to progesterone could be used for the immunodetection of milk and serum progesterone levels. We report here the development of hybrid cells prdoducing monoclonal antibodies specific for progesterone using hybridoma technology. Hybridoma cells secreting monoclonal antibodies against progesterone (MAM 2H11, MAM 6G6) were developed by fusion of the spleen cells of an immunized BALB/c mouse with FO (ATCC CRL 1646) mouse myeloma cells. These monoclonal antibodies were characterized for use in the development of diagnostic kits based on the enzyme-linked immunosorbent assay (ELISA) test system. ELISA tests showed that antibodies produced by 2 hybrids from 375 wells reacted specifically with progesterone. Monoclonal antibodies did not show any cross reaction with other steroid hormones.
Birincil Dil tr
Bölüm Makaleler
Yazarlar

Yazar: Fatıma YÜCEL

Tarihler

Yayımlanma Tarihi : 1 Nisan 1999

Bibtex @ { tbtkbiology140267, journal = {Turkish Journal of Biology}, issn = {1300-0152}, eissn = {1303-6092}, address = {}, publisher = {TÜBİTAK}, year = {1999}, volume = {23}, pages = {393 - 400}, doi = {}, title = {Production of Monoclonal Antibodies specific for Progesterone}, key = {cite}, author = {YÜCEL, Fatıma} }
APA YÜCEL, F . (1999). Production of Monoclonal Antibodies specific for Progesterone. Turkish Journal of Biology , 23 (4) , 393-400 . Retrieved from https://dergipark.org.tr/tr/pub/tbtkbiology/issue/11750/140267
MLA YÜCEL, F . "Production of Monoclonal Antibodies specific for Progesterone". Turkish Journal of Biology 23 (1999 ): 393-400 <https://dergipark.org.tr/tr/pub/tbtkbiology/issue/11750/140267>
Chicago YÜCEL, F . "Production of Monoclonal Antibodies specific for Progesterone". Turkish Journal of Biology 23 (1999 ): 393-400
RIS TY - JOUR T1 - Production of Monoclonal Antibodies specific for Progesterone AU - Fatıma YÜCEL Y1 - 1999 PY - 1999 N1 - DO - T2 - Turkish Journal of Biology JF - Journal JO - JOR SP - 393 EP - 400 VL - 23 IS - 4 SN - 1300-0152-1303-6092 M3 - UR - Y2 - 2020 ER -
EndNote %0 Turkish Journal of Biology Production of Monoclonal Antibodies specific for Progesterone %A Fatıma YÜCEL %T Production of Monoclonal Antibodies specific for Progesterone %D 1999 %J Turkish Journal of Biology %P 1300-0152-1303-6092 %V 23 %N 4 %R %U
ISNAD YÜCEL, Fatıma . "Production of Monoclonal Antibodies specific for Progesterone". Turkish Journal of Biology 23 / 4 (Nisan 1999): 393-400 .
AMA YÜCEL F . Production of Monoclonal Antibodies specific for Progesterone. Turkish Journal of Biology. 1999; 23(4): 393-400.
Vancouver YÜCEL F . Production of Monoclonal Antibodies specific for Progesterone. Turkish Journal of Biology. 1999; 23(4): 400-393.