Yıl 2015, Cilt 45 , Sayı 6, Sayfalar 1329 - 1334 2015-12-04

Applicability of Xpert MTB/RIF assay for routine diagnosis of tuberculosis: a four-year single-center experience
Applicability of Xpert MTB/RIF assay for routine diagnosis of tuberculosis: a four-year single-center experience

CEM ÇELİK [1] , MUSTAFA GÖKHAN GÖZEL [2] , MUSTAFA ZAHİR BAKICI [3] , SERDAR BERK [4] , SEFA LEVENT ÖZŞAHİN [5] , ESRA GÜLTÜRK [6]


Background/aim: The aim of this study is to assess the Xpert MTB/RIF assay for diagnosis of the Mycobacterium tuberculosis complex in clinical samples and to compare the results by reference to the diagnostic method, Bactec MGIT 960. Materials and methods: A total of 7407 samples were included from patients not primarily suggesting pulmonary or extrapulmonary tuberculosis (TB), collected from patients required to be screened for TB and excluding TB diagnoses since it was a differential diagnosis. Also included were a total of 411 samples from patients primarily suggesting pulmonary or extrapulmonary TB. Results: In the first group, 152 of 7407 samples yielded positive results with the Bactec MGIT 960, 131 (1.77%) were found positive with Löwenstein-Jensen medium, and 295 (3.99%) were found positive with Ziehl-Neelsen staining. In the second group, 24 (5.8%), 17 (4.1%), and 28 (6.8%) of 411 samples were found positive. Xpert MTB/RIF [27 (6.6%) of 411 samples] detected 3 additional samples as positive, and these 3 cases were clinically compatible with TB. Conclusion: The Xpert MTB/RIF assay shows superior performance for the diagnosis of TB. Its usefulness in culture-negative patients and the best method for integrating this diagnostic method into current tuberculosis diagnostic algorithms both need further study.
Background/aim: The aim of this study is to assess the Xpert MTB/RIF assay for diagnosis of the Mycobacterium tuberculosis complex in clinical samples and to compare the results by reference to the diagnostic method, Bactec MGIT 960. Materials and methods: A total of 7407 samples were included from patients not primarily suggesting pulmonary or extrapulmonary tuberculosis (TB), collected from patients required to be screened for TB and excluding TB diagnoses since it was a differential diagnosis. Also included were a total of 411 samples from patients primarily suggesting pulmonary or extrapulmonary TB. Results: In the first group, 152 of 7407 samples yielded positive results with the Bactec MGIT 960, 131 (1.77%) were found positive with Löwenstein-Jensen medium, and 295 (3.99%) were found positive with Ziehl-Neelsen staining. In the second group, 24 (5.8%), 17 (4.1%), and 28 (6.8%) of 411 samples were found positive. Xpert MTB/RIF [27 (6.6%) of 411 samples] detected 3 additional samples as positive, and these 3 cases were clinically compatible with TB. Conclusion: The Xpert MTB/RIF assay shows superior performance for the diagnosis of TB. Its usefulness in culture-negative patients and the best method for integrating this diagnostic method into current tuberculosis diagnostic algorithms both need further study.
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Birincil Dil tr
Bölüm Makaleler
Yazarlar

Yazar: CEM ÇELİK

Yazar: MUSTAFA GÖKHAN GÖZEL

Yazar: MUSTAFA ZAHİR BAKICI

Yazar: SERDAR BERK

Yazar: SEFA LEVENT ÖZŞAHİN

Yazar: ESRA GÜLTÜRK

Tarihler

Yayımlanma Tarihi : 4 Aralık 2015

Bibtex @ { tbtkmedical149020, journal = {Turkish Journal of Medical Sciences}, issn = {1300-0144}, eissn = {1303-6165}, address = {}, publisher = {TÜBİTAK}, year = {2015}, volume = {45}, pages = {1329 - 1334}, doi = {}, title = {Applicability of Xpert MTB/RIF assay for routine diagnosis of tuberculosis: a four-year single-center experience}, key = {cite}, author = {ÇELİK, CEM and GÖZEL, MUSTAFA GÖKHAN and BAKICI, MUSTAFA ZAHİR and BERK, SERDAR and ÖZŞAHİN, SEFA LEVENT and GÜLTÜRK, ESRA} }
APA ÇELİK, C , GÖZEL, M , BAKICI, M , BERK, S , ÖZŞAHİN, S , GÜLTÜRK, E . (2015). Applicability of Xpert MTB/RIF assay for routine diagnosis of tuberculosis: a four-year single-center experience. Turkish Journal of Medical Sciences , 45 (6) , 1329-1334 . Retrieved from https://dergipark.org.tr/tr/pub/tbtkmedical/issue/12391/149020
MLA ÇELİK, C , GÖZEL, M , BAKICI, M , BERK, S , ÖZŞAHİN, S , GÜLTÜRK, E . "Applicability of Xpert MTB/RIF assay for routine diagnosis of tuberculosis: a four-year single-center experience". Turkish Journal of Medical Sciences 45 (2015 ): 1329-1334 <https://dergipark.org.tr/tr/pub/tbtkmedical/issue/12391/149020>
Chicago ÇELİK, C , GÖZEL, M , BAKICI, M , BERK, S , ÖZŞAHİN, S , GÜLTÜRK, E . "Applicability of Xpert MTB/RIF assay for routine diagnosis of tuberculosis: a four-year single-center experience". Turkish Journal of Medical Sciences 45 (2015 ): 1329-1334
RIS TY - JOUR T1 - Applicability of Xpert MTB/RIF assay for routine diagnosis of tuberculosis: a four-year single-center experience AU - CEM ÇELİK , MUSTAFA GÖKHAN GÖZEL , MUSTAFA ZAHİR BAKICI , SERDAR BERK , SEFA LEVENT ÖZŞAHİN , ESRA GÜLTÜRK Y1 - 2015 PY - 2015 N1 - DO - T2 - Turkish Journal of Medical Sciences JF - Journal JO - JOR SP - 1329 EP - 1334 VL - 45 IS - 6 SN - 1300-0144-1303-6165 M3 - UR - Y2 - 2019 ER -
EndNote %0 Turkish Journal of Medical Sciences Applicability of Xpert MTB/RIF assay for routine diagnosis of tuberculosis: a four-year single-center experience %A CEM ÇELİK , MUSTAFA GÖKHAN GÖZEL , MUSTAFA ZAHİR BAKICI , SERDAR BERK , SEFA LEVENT ÖZŞAHİN , ESRA GÜLTÜRK %T Applicability of Xpert MTB/RIF assay for routine diagnosis of tuberculosis: a four-year single-center experience %D 2015 %J Turkish Journal of Medical Sciences %P 1300-0144-1303-6165 %V 45 %N 6 %R %U
ISNAD ÇELİK, CEM , GÖZEL, MUSTAFA GÖKHAN , BAKICI, MUSTAFA ZAHİR , BERK, SERDAR , ÖZŞAHİN, SEFA LEVENT , GÜLTÜRK, ESRA . "Applicability of Xpert MTB/RIF assay for routine diagnosis of tuberculosis: a four-year single-center experience". Turkish Journal of Medical Sciences 45 / 6 (Aralık 2015): 1329-1334 .
AMA ÇELİK C , GÖZEL M , BAKICI M , BERK S , ÖZŞAHİN S , GÜLTÜRK E . Applicability of Xpert MTB/RIF assay for routine diagnosis of tuberculosis: a four-year single-center experience. Turkish Journal of Medical Sciences. 2015; 45(6): 1329-1334.
Vancouver ÇELİK C , GÖZEL M , BAKICI M , BERK S , ÖZŞAHİN S , GÜLTÜRK E . Applicability of Xpert MTB/RIF assay for routine diagnosis of tuberculosis: a four-year single-center experience. Turkish Journal of Medical Sciences. 2015; 45(6): 1334-1329.