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Ratlarda Deneysel Olarak Oluşturulan Postoperatif Peritoneal Adezyonları Önlemede %50 Dekstrozun Rolü

Yıl 2022, Cilt: 16 Sayı: 3, 220 - 224, 30.05.2022
https://doi.org/10.12956/tchd.888601

Öz

Amaç: Postoperatif peritoneal adezyonları (PPA) önlemek için pek çok değişik yöntem ve uygulama araştırılmıştır. Bu uygulamalardan biri de hipertonik solüsyonlarla peritoneal boşlukta su miktarını artırarak peritoneal yapışıklıkların önlenmesidir. Bu çalışmada, PPA oluşmasının önlenmesinde %50 dekstroz çözeltisinin rolünün araştırılması planlanmıştır.

Gereç ve Yöntemler: Bu çalışmada, %50 dekstroz, sham ve kontrol grubu olmak üzere her biri on ikişer rattan oluşan üç ayrı grup oluşturuldu. Ratlar on dördüncü gün sakrifiye edilip relaparotomileri yapıldı. Sonuçlar daha önceden tanımlanmış klasifikasyonlara uygun makroskopik ve mikroskopik açıdan değerlendirildi.

Bulgular: %50 dekstroz ile tedavi edilen grup, makroskopik (Nair) ve mikroskopik (Zuhlke) sınıflandırmalarına göre kontrol grubu ile karşılaştırıldığında PPA gelişimi açısından belirgin farklılık tespit edilmedi.

Sonuç: Ratlarda deneysel oluşturulan adezyon modelinde, %50 dekstroz solüsyonunun PPA oluşumunu önleme üzerinde etkisi kanıtlanamamıştır.

Kaynakça

  • Herrick SE, Mutsaers SE, Ozua P, Sulaiman H, Omer A, Boulos P, et al. Human peritoneal adhesions are highly cellular, innervated, and vascularized. J Pathol 2000;192:67-72.
  • Makarchian HR, Kasraianfard A, Ghaderzadeh P, Reza Javadi SM, Ghorbanpoor M. The effectiveness of heparin, platelet-rich plasma (PRP), and silver nanoparticles on prevention of postoperative peritoneal adhesion formation in rats. Acta Cir Bras 2017; 32:22-7.
  • Arung W, P Drion, O Detry. Sepramesh and postoperative peritoneal adhesions in a rat model. Acta Chir Belg 2016;116:357-61.
  • Chen CH, Chen SH, Mao SH, Tsai MJ, Chou PY, Liao CH, et al. Injectable thermosensitive hydrogel containing hyaluronic acid and chitosan as a barrier for prevention of postoperative peritoneal adhesion. Carbohydr Polym 2017;173:721-31.
  • Du XH, Liu JQ, Xin K, Liu GH. Dexamethasone and sodium carboxymethyl cellulose prevent postoperative intraperitoneal adhesions in rats. Braz J Med Biol Res 2015;48:344-8.
  • Gao Q, Wei G, Wu Y, Yao N, Zhou C, Wang K, et al. Paeoniflorin prevents postoperative peritoneal adhesion formation in an experimental rat model. Oncotarget 2017;8:93899-911.
  • Goret CC, Goret NE, Kiraz A, Ozkan OF, Karaayvaz M. The effect of pycnogenol on lymphatic nodes and adhesion during in a peritoneal adhesion model in rats. Acta Cir Bras 2018;33:134-43.
  • Hellebrekers BW, Trimbos Kemper TC, Trimbos JB, Kooistra T. Use of fibrinolytic agents in the prevention of postoperative adhesion formation. Fertil Steril 2000;4:203-12.
  • diZerega GS, Verco SJS, Young P, Kettel M, Kobak W, Sanfilippo J, et al. A randomized, controlled pilot study of the safety and efficacy of 4% icodextrin solution in the reduction of adhesions following laparoscopic gynaecological surgery. Hum Reprod 2002;17: 1031-8.
  • Duffy DM, GS diZerega. Adhesion controversies: pelvic pain as a cause of adhesions, crystalloids in preventing them. J Reprod Med 1996;41:19-26.
  • DeCherney AH, GS diZerega. Clinical problem of intraperitoneal postsurgical adhesion formation following general surgery and the use of adhesion prevention barriers. Surg Clin North Am 1997;77:671-88.
  • Nair SK, Bhat K, Aurora AL. Role of proteolyticenzyme in the prevention of postoperative intraperitoneal adhesions. Arch Surg 1974;108:849–53.
  • Zühlke HV, Lorenz EM, Straub EM, Savvas V. Pathophysiology and classification of adhesions. Langenbecks Arch Chir Suppl Verh Dtsch Ges Chir 1990;1009–16.
  • Stangel JJ, JD Nisbet, Settles H. Formation and prevention of postoperative abdominal adhesions. J Reprod Med 1984;29:143-56.
  • Flessner MF, Dedrick RL, Reynolds JC. Bidirectional peritoneal transport of immunoglobulin in rats: tissue concentration profiles. Am J Physiol 1992; 263: F15-23.
  • Garrett CG, Soto J, Riddick J, Billante CR, Reninisch L. Effect of mitomycin-C on vocal fold healing in a canine model. Ann Otol Rhinol Laryngol 2001;110:25-30.
  • Graziani F, Ivanoski, Cei S, Ducci F, Tonetti M, Gabriele M. The in vitro effect of different PRP concentrations on osteoblasts and fibroblasts. Clin Oral Implants Res 2006;17:212-9.

The Role of 50% Dekstrose For Preventing Postoperative Peritoneal Adhesions in Adhesive Intestinal Obstruction in Rats

Yıl 2022, Cilt: 16 Sayı: 3, 220 - 224, 30.05.2022
https://doi.org/10.12956/tchd.888601

Öz

Objective: Various methods have been investigated to prevent postoperative peritoneal adhesions. It is thought that hypertonic solutions could be used to prevent peritoneal adhesions with waterisation. The aim of this study was to investigate the role of 50% dekstrose solution in the formation of PPA.

Material and Methods: Rats were divided into 3 groups, each consisting of twelve rats. Groups were classified as; 50% dekstrose, sham and control group. The rats were sacrified on the 14th day and relaparotomies were performed. The results were evaluated macroscopically and microscopically according to predetermined classifications.


Results:
When the groups treated with 50% dekstrose were compared with the control groups according to the previously described macroscopic (Nair) and microscopic (Zuhlke) classifications, there was no significant difference in PPA formation both microscopically and macroscopically.


Conclusion:
In the experimentally created adhesion model, the effect of 50% dekstrose on preventing PPA formation could not be proved.

Kaynakça

  • Herrick SE, Mutsaers SE, Ozua P, Sulaiman H, Omer A, Boulos P, et al. Human peritoneal adhesions are highly cellular, innervated, and vascularized. J Pathol 2000;192:67-72.
  • Makarchian HR, Kasraianfard A, Ghaderzadeh P, Reza Javadi SM, Ghorbanpoor M. The effectiveness of heparin, platelet-rich plasma (PRP), and silver nanoparticles on prevention of postoperative peritoneal adhesion formation in rats. Acta Cir Bras 2017; 32:22-7.
  • Arung W, P Drion, O Detry. Sepramesh and postoperative peritoneal adhesions in a rat model. Acta Chir Belg 2016;116:357-61.
  • Chen CH, Chen SH, Mao SH, Tsai MJ, Chou PY, Liao CH, et al. Injectable thermosensitive hydrogel containing hyaluronic acid and chitosan as a barrier for prevention of postoperative peritoneal adhesion. Carbohydr Polym 2017;173:721-31.
  • Du XH, Liu JQ, Xin K, Liu GH. Dexamethasone and sodium carboxymethyl cellulose prevent postoperative intraperitoneal adhesions in rats. Braz J Med Biol Res 2015;48:344-8.
  • Gao Q, Wei G, Wu Y, Yao N, Zhou C, Wang K, et al. Paeoniflorin prevents postoperative peritoneal adhesion formation in an experimental rat model. Oncotarget 2017;8:93899-911.
  • Goret CC, Goret NE, Kiraz A, Ozkan OF, Karaayvaz M. The effect of pycnogenol on lymphatic nodes and adhesion during in a peritoneal adhesion model in rats. Acta Cir Bras 2018;33:134-43.
  • Hellebrekers BW, Trimbos Kemper TC, Trimbos JB, Kooistra T. Use of fibrinolytic agents in the prevention of postoperative adhesion formation. Fertil Steril 2000;4:203-12.
  • diZerega GS, Verco SJS, Young P, Kettel M, Kobak W, Sanfilippo J, et al. A randomized, controlled pilot study of the safety and efficacy of 4% icodextrin solution in the reduction of adhesions following laparoscopic gynaecological surgery. Hum Reprod 2002;17: 1031-8.
  • Duffy DM, GS diZerega. Adhesion controversies: pelvic pain as a cause of adhesions, crystalloids in preventing them. J Reprod Med 1996;41:19-26.
  • DeCherney AH, GS diZerega. Clinical problem of intraperitoneal postsurgical adhesion formation following general surgery and the use of adhesion prevention barriers. Surg Clin North Am 1997;77:671-88.
  • Nair SK, Bhat K, Aurora AL. Role of proteolyticenzyme in the prevention of postoperative intraperitoneal adhesions. Arch Surg 1974;108:849–53.
  • Zühlke HV, Lorenz EM, Straub EM, Savvas V. Pathophysiology and classification of adhesions. Langenbecks Arch Chir Suppl Verh Dtsch Ges Chir 1990;1009–16.
  • Stangel JJ, JD Nisbet, Settles H. Formation and prevention of postoperative abdominal adhesions. J Reprod Med 1984;29:143-56.
  • Flessner MF, Dedrick RL, Reynolds JC. Bidirectional peritoneal transport of immunoglobulin in rats: tissue concentration profiles. Am J Physiol 1992; 263: F15-23.
  • Garrett CG, Soto J, Riddick J, Billante CR, Reninisch L. Effect of mitomycin-C on vocal fold healing in a canine model. Ann Otol Rhinol Laryngol 2001;110:25-30.
  • Graziani F, Ivanoski, Cei S, Ducci F, Tonetti M, Gabriele M. The in vitro effect of different PRP concentrations on osteoblasts and fibroblasts. Clin Oral Implants Res 2006;17:212-9.
Toplam 17 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Cerrahi
Bölüm ORIGINAL ARTICLES
Yazarlar

Gökhan Demirtaş 0000-0003-0787-2330

Süleyman Arif Bostancı 0000-0002-7512-3895

Doğuş Güney 0000-0001-7168-2123

Sema Hücümenoğlu Bu kişi benim 0000-0002-6898-4101

Tuğrul Tiryaki 0000-0002-9544-1137

Yayımlanma Tarihi 30 Mayıs 2022
Gönderilme Tarihi 14 Haziran 2021
Yayımlandığı Sayı Yıl 2022 Cilt: 16 Sayı: 3

Kaynak Göster

Vancouver Demirtaş G, Bostancı SA, Güney D, Hücümenoğlu S, Tiryaki T. Ratlarda Deneysel Olarak Oluşturulan Postoperatif Peritoneal Adezyonları Önlemede %50 Dekstrozun Rolü. Türkiye Çocuk Hast Derg. 2022;16(3):220-4.

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