Bazı Fumaria L. (Papaveraceae) Türlerinin Metanolik Ekstraktlarının Kanser Hücre Hatları Üzerindeki İn Vitro Sitotoksik Etkileri

Öz

Fumaria cinsi (Papaveraceae) halk arasında “Şahtere” olarak bilinir. Son derece zengin bileşiklere sahip olduğu kanıtlanmış olan cins, halk arasında birçok rahatsızlığın tedavisinde kullanılmaktadır. Bu çalışmada, Fumaria asepala Boiss. ve Fumaria schleicheri Soy.-Will. subsp. microcarpa Boiss. ex Hausskn. taksonlarının toprak üstü kısımlarının metanolik ekstraktının Beas-2B, sh-sy5, HCT116 ve A549 hücre hatlarına karşı sitotoksik özellikleri araştırıldı. Kanser hücrelerinin canlılığındaki değişiklikler 3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolyum bromür (MTT) yöntemi kullanılarak tespit edildi. Sonuçlar, F. asepala ve F. schichleiri subsp. microcarpa’nın metanolik ekstraktının özellikle kolon (HCT-116) kanseri hücrelerinin hücre canlılığında istatistiksel olarak anlamlı bir azalma olduğunu gösterirken, akciğer (A549) kanseri ve beyin (sh-sy5) kanseri hücreleri üzerinde sitotoksik etkinlik sağlamamıştır. Ayrıca bitkisel ekstraktlar, sağlıklı akciğer hücreleri (Beas-2B) üzerinde ise herhangi bir toksik etki yaratmamıştır.

Anahtar Kelimeler

• Fumaria
• Sitotoksik
• Beas-2B
• sh-sy5
• HCT116
• A549

In Vitro Cytotoxic Effects of Some Fumaria L. (Papaveraceae) Species Methanolic Extracts on Cancer Cell Lines

Abstract

The Fumaria L. species (Papaveraceae), is known colloquially as “Şahtere”. This species, which has been proven to contain significantly rich components, is used by people to treat a number of different diseases. This study investigated the cytotoxic effects of the methanolic extracts of above-ground portions of Fumaria asepala Boiss. and Fumaria schleicheri Soy.-Will. subsp. microcarpa Boiss. ex Hausskn. taxons against Beas-2B, sh-sy5, HCT116 and A549 cell lines. The changes in cancer cell vitality were identified using the 3-(4.5-dimethylthiazol -2-il)-2.5-diphenyltetrazolium bromide (MTT). While the results showed that F. asepala and F. schleicheri subsp. microcarpa's methanolic extracts presented a meaningful decrease in the vitality of colon (HCT-116) cancer cells, no cytotoxic effect was achieved on lung (A549) cancer and brain (sh-sy5) cancer cells. Also, the herbal extracts did not create any toxic effect on healthy lung cells (Beas-2B).

Keywords

• Fumaria
• Cytotoxic
• Beas-2B
• sh-sy5
• HCT116
• A549

Kaynakça

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