In this study, a nanofiber surface loaded with apheresis platelet concentrate (APC) was produced for the first time to develop a bioactive wound dressing design. Nanofiber surface (n=5) consisting of polyurethane polymer outer layer, polyvinyl alcohol polymer middle layer, and polycaprolactone polymer matrix inner layer were produced via the electrospinning method. The surface morphologies of the produced nanofiber surfaces were examined by scanning electron microscopy. Quantitative analyzes of growth factors released from the APC-loaded composite nanofiber surfaces into phosphate-buffered saline at certain time intervals were performed with the ELISA. When the release amounts between bFGF, EGF, and PDGF-AA groups were compared, a significant difference was found in all periods (p<0.05). When the time-dependent release changes of each group were examined, there was no statistically significant difference in the bFGF group (p>0.05), but there was a significant difference between the EGF and PDGF-AA growth factors (p<0.05).
1. Jiang Y, Huang S, Fu X, Liu H, Ran X, Lu S, Hu D, Li Q, Zhang H, Li Y, Wang R. 2011. Epidemiology of chronic cutaneous wounds in China. Wound Repair and Regeneration 19(2), 181-188.
2. Sen CK, Gordillo GM, Roy S, Kirsner R, Lambert L, Hunt TK, Gottrup F, Gurtner GC, Longaker MT. 2009. Human skin wounds: A major and snowballing threat to public health and the economy. Wound Repair and Regeneration 17(6), 763-771.
3. Broussard KC, Powers JG. 2013. Wound dressings: Selecting the most appropriate type. American Journal of Clinical Dermatology 14(6), 449-59.
4. Simões D, Miguel SP, Ribeiro MP, Coutinho P, Mendonça AG, Correia IJ. 2018. Recent advances on antimicrobial wound dressing: A review. European Journal of Pharmaceutics and Biopharmaceutics 1(127), 130-141.
5. Chang CJ, Kazemzadeh-Narbat M. 2021. Innovation in wound care products: a FDA regulatory perspective. Journal of Wound Care 30(Sup2), 3-4.
6. Chermnykh ES, Kiseleva EV, Rogovaya OS, Rippa AL, Vasiliev AV, Vorotelyak EA. 2018. Tissue-engineered biological dressing accelerates skin wound healing in mice via formation of provisional connective tissue. Histology and Histopathology from Cell Biology to Tissue Engineering 33,1189-1199.
7. Stupin VA, Gabitov RB, Sinelnikova TG, Silina EV. 2018. Biological mechanisms of chronic wound and diabetic foot healing: The role of collagen. Serbian Journal of Experimental and Clinical Research 19(4), 373-82.
8. Langer C, Mahajan V. 2014. Platelet-rich plasma in dermatology. JK Science 16(4), 147.
9. Foster TE, Puskas BL, Mandelbaum BR, Gerhardt MB, Rodeo SA. 2009. Platelet-rich plasma: From basic science to clinical applications. The American Journal of Sports Medicine 37(11):2259-2272.
10. Gholami GA, Mohammadi M, Abrishami MR. 2014. Platelet rich plasma: Review of literature. Journal of Dental School 32(3), 176-186.
11. Dvorak HF, Brown LF, Detmar M, Dvorak AM. 1995. Vascular permeability factor/vascular endothelial growth factor, microvascular hyperpermeability, and angiogenesis. The American Journal of Pathology 146(5), 1029.
12. Cohen S, Carpenter G. 1975. Human epidermal growth factor: isolation and chemical and biological properties. Proceedings of the National Academy of Sciences 72(4), 1317-1321.
13. Norouzi M, Boroujeni SM, Omidvarkordshouli N, Soleimani M. 2015. Advances in skin regeneration: application of electrospun scaffolds. Advanced Healthcare Materials 4(8), 1114-1133.
14. Garcia-Orue I, Gainza G, Gutierrez FB, Aguirre JJ, Evora C, Pedraz JL, Hernandez RM, Delgado A, Igartua M. 2017. Novel nanofibrous dressings containing rhEGF and Aloe vera for wound healing applications. International Journal of Pharmaceutics 523(2), 556-566.
15. Wang Z, Qian Y, Li L, Pan L, Njunge LW, Dong L, Yang L. 2016. Evaluation of emulsion electrospun polycaprolactone/hyaluronan/ epidermal growth factor nanofibrous scaffolds for wound healing. Journal of Biomaterials Applications 30(6), 686-698.
16. Jang, C. S., Kim, S. I., Kim, H. K., Kweon, C. O., Kim, B. W., Kim, D. C., ... & Ryu, J. K. (2014). Plateletpheresis: The process, devices, and indicators of product quality. Journal of Life Science 24(9), 1030-1038.
17. McCullough, J. 2010. Overview of platelet transfusion. In Seminars in Hematology 47(3), 235-242. WB Saunders.
18. El-Timamy A, El Sharaby F, Eid F, El Dakroury A, Mostafa Y, Shaker O. 2020. Effect of platelet-rich plasma on the rate of orthodontic tooth movement: a split-mouth randomized trial. The Angle Orthodontist 90(3), 354-361.
19. Li J, Chen M, Wei X, Hao Y, Wang J. 2017. Evaluation of 3D-printed polycaprolactone scaffolds coated with freeze-dried platelet-rich plasma for bone regeneration. Materials 10(7), 831.
20. Zhang X, Yao D, Zhao W, Zhang R, Yu B, Ma G, Li Y, Hao D, Xu FJ. 2021. Engineering Platelet‐Rich Plasma Based Dual‐Network Hydrogel as a Bioactive Wound Dressing with Potential Clinical Translational Value. Advanced Functional Materials 31(8), 2009258.
21. Farzamfar S, Esmailpour F, Rahmati M, Vaez A, Mirzaii M, Garmabi B, Shayannia A, Ebrahimi E, Vahedi H, Salehi M. 2017. Poly-lactic acid/gelatin nanofiber (PLA/GTNF) conduits containing platelet-rich plasma for peripheral nerve regeneration. International Journal of Health Studies 3(2), 18.
22. Miroshnichenko S, Timofeeva V, Permyakova E, Ershov S, Kiryukhantsev-Korneev P, Dvořaková E, Shtansky DV, Zajíčková L, Solovieva A, Manakhov A. 2019. Plasma-coated polycaprolactone nanofibers with covalently bonded platelet-rich plasma enhance adhesion and growth of human fibroblasts. Nanomaterials 9(4), 637.
23. Unnithan AR, Barakat NA, Pichiah PT, Gnanasekaran G, Nirmala R, Cha YS, Jung CH, El-Newehy M, Kim HY. 2012. Wound-dressing materials with antibacterial activity from electrospun polyurethane–dextran nanofiber mats containing ciprofloxacin HCl. Carbohydrate Polymers 90(4), 1786-1793.
24. Alavarse AC, de Oliveira Silva FW, Colque JT, da Silva VM, Prieto T, Venancio EC, Bonvent JJ. 2017. Tetracycline hydrochloride-loaded electrospun nanofibers mats based on PVA and chitosan for wound dressing. Materials Science and Engineering: C 77, 271-281.
25. Choi JI, Kim MS, Chung GY, Shin HS. 2017. Spirulina extract-impregnated alginate-PCL nanofiber wound dressing for skin regeneration. Biotechnology and Bioprocess Engineering 22, 679-685.
26. Hsieh YL. 2000. Mat Characteristics of Polyester Fibers. Pastore CM, Kiekens P (Ed), Mat Characteristics of Fibers and Textiles. New York: Markel Dekker Inc. p.33-57.
27. Cordenonsi LM, Faccendini A, Rossi S, Bonferoni MC, Malavasi L, Raffin R, ... & Ferrari F. 2019. Platelet lysate loaded electrospun scaffolds: Effect of nanofiber types on wound healing. European Journal of Pharmaceutics and Biopharmaceutics, 142, 247-257.
28. Karuppannan SK, Dowlath MJ, Ramalingam R, Musthafa SA, Ganesh MR, Chithra V, Ravindran B, Arunachalam KD. 2022. Quercetin functionalized hybrid electrospun nanofibers for wound dressing application. Materials Science and Engineering: B 285, 115933.
29. Tanha S, Rafiee‐Tehrani M, Abdollahi M, Vakilian S, Esmaili Z, Naraghi ZS, Seyedjafari E, Javar HA. 2017. G‐CSF loaded nanofiber/nanoparticle composite coated with collagen promotes wound healing in vivo. Journal of Biomedical Materials Research Part A 105(10), 2830-2842.
30. Kim SE, Heo DN, Lee JB, Kim JR, Park SH, Jeon SH, Kwon IK. 2009. Electrospun gelatin/polyurethane blended nanofibers for wound healing. Biomedical Materials 4(4), 044106.
31. Yang Y, Hu H. 2017. Spacer fabric-based exuding wound dressing–Part II: Comparison with commercial wound dressings. Textile Research Journal 87(12), 1481-1493.
32. Zhang C, Yuan X, Wu L, Han Y, Sheng J. 2005. Study on morphology of electrospun poly (vinyl alcohol) mats. European Polymer Journal 41(3), 423-432.
33. Hashmi M, Ullah S, Ullah A, Khan MQ, Hussain N, Khatri M, Bie X, Lee J, Kim IS. 2020. An optimistic approach “from hydrophobic to super hydrophilic nanofibers” for enhanced absorption properties. Polymer Testing 90, 106683.
34. Zhao JH, Xu L, Liu Q. 2015. Effect of ethanol post-treatment on the bubble-electrospun poly (vinyl alcohol) nanofiber. Thermal Science 19(4), 1353-1356.
35. Selçuk E, Calapoğlu NŞ. 2022. Overview of primary messengers and their receptors. Suleyman Demirel University Journal of Health Sciences 13(3), 559-566.
36. Fredenberg S, Wahlgren M, Reslow M, Axelsson A. 2011. The mechanisms of drug release in poly (lactic-co-glycolic acid)-based drug delivery systems--a review. International Journal of Pharmaceutics 415(1-2), 34–52.
37. Shah SS, Cha Y, Pitt CG. 1992. Poly (glycolic acid-co-dl-lactic acid): diffusion or degradation-controlled drug delivery? Journal of Controlled Release 18(3), 261-270.
38. Kajdič S, Planinšek O, Gašperlin M, Kocbek P. 2019. Electrospun nanofibers for customized drug-delivery systems. Journal of Drug Delivery Science and Technology 51, 672-681.
39. Laha A, Sharma CS, Majumdar S. 2017. Sustained drug release from multi-layered sequentially crosslinked electrospun gelatin nanofiber mesh. Materials Science and Engineering: C 76, 782-786.
40. Li T, Ding X, Tian L, Hu J, Yang X, Ramakrishna S. 2017. The control of beads diameter of bead-on-string electrospun nanofibers and the corresponding release behaviors of embedded drugs. Materials Science and Engineering: C 74, 471-477.
41. Ceylan M, Yang SY, Asmatulu R. 2017. Effects of gentamicin-loaded PCL nanofibers on growth of Gram positive and Gram-negative bacteria. International Journal of Applied Microbiology and Biotechnology Research 5, 40-51.
42. Arnoczky SP, Shebani-Rad S. 2013. The basic science of platelet-rich plasma (PRP): What clinicians need to know? Sports Medicine and Arthroscopy Review 21(4), 180-185.
43. Wang Q, Qian Z, Liu B, Liu J, Zhang L, Xu J. 2019. In vitro and in vivo evaluation of new PRP antibacterial moisturizing dressings for infectious wound repair. Journal of Biomaterials Science, Polymer Edition 30(6), 462-485.
44. Cheng H, Yang X, Che X, Yang M, Zhai G. 2018. Biomedical application and controlled drug release of electrospun fibrous materials. Materials Science and Engineering:C 90, 750-763.
45. Diaz-Gomez L, Alvarez-Lorenzo C, Concheiro A, Silva M, Dominguez F, Sheikh FA, Cantu T, Desai R, Garcia VL, Macossay J. 2014. Biodegradable electrospun nanofibers coated with platelet-rich plasma for cell adhesion and proliferation. Materials Science and Engineering:C 40, 180-188.
46. Üstündağ GC, Karaca E, Özbek SE, Çavuşoğlu İ. 2010. In vivo evaluation of electrospun poly (vinyl alcohol) /sodium alginate nanofibrous mat as wound dressing. Tekstil ve Konfeksiyon 20(4), 290-298
Year 2024,
Volume: 34 Issue: 3, 244 - 252, 30.09.2024
Bursa Uludağ Üniversitesi Bilimsel Araştırma Proje Koordinatörlüğü tarafından desteklenmiştir.
Project Number
THIZ-2021-471
Thanks
Bursa Uludağ Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğüne ve Komisyon üyelerine (THIZ-2021-471) finansal destek sağladıkları için;
TEŞEKKÜRLERİMİ sunarım.
References
1. Jiang Y, Huang S, Fu X, Liu H, Ran X, Lu S, Hu D, Li Q, Zhang H, Li Y, Wang R. 2011. Epidemiology of chronic cutaneous wounds in China. Wound Repair and Regeneration 19(2), 181-188.
2. Sen CK, Gordillo GM, Roy S, Kirsner R, Lambert L, Hunt TK, Gottrup F, Gurtner GC, Longaker MT. 2009. Human skin wounds: A major and snowballing threat to public health and the economy. Wound Repair and Regeneration 17(6), 763-771.
3. Broussard KC, Powers JG. 2013. Wound dressings: Selecting the most appropriate type. American Journal of Clinical Dermatology 14(6), 449-59.
4. Simões D, Miguel SP, Ribeiro MP, Coutinho P, Mendonça AG, Correia IJ. 2018. Recent advances on antimicrobial wound dressing: A review. European Journal of Pharmaceutics and Biopharmaceutics 1(127), 130-141.
5. Chang CJ, Kazemzadeh-Narbat M. 2021. Innovation in wound care products: a FDA regulatory perspective. Journal of Wound Care 30(Sup2), 3-4.
6. Chermnykh ES, Kiseleva EV, Rogovaya OS, Rippa AL, Vasiliev AV, Vorotelyak EA. 2018. Tissue-engineered biological dressing accelerates skin wound healing in mice via formation of provisional connective tissue. Histology and Histopathology from Cell Biology to Tissue Engineering 33,1189-1199.
7. Stupin VA, Gabitov RB, Sinelnikova TG, Silina EV. 2018. Biological mechanisms of chronic wound and diabetic foot healing: The role of collagen. Serbian Journal of Experimental and Clinical Research 19(4), 373-82.
8. Langer C, Mahajan V. 2014. Platelet-rich plasma in dermatology. JK Science 16(4), 147.
9. Foster TE, Puskas BL, Mandelbaum BR, Gerhardt MB, Rodeo SA. 2009. Platelet-rich plasma: From basic science to clinical applications. The American Journal of Sports Medicine 37(11):2259-2272.
10. Gholami GA, Mohammadi M, Abrishami MR. 2014. Platelet rich plasma: Review of literature. Journal of Dental School 32(3), 176-186.
11. Dvorak HF, Brown LF, Detmar M, Dvorak AM. 1995. Vascular permeability factor/vascular endothelial growth factor, microvascular hyperpermeability, and angiogenesis. The American Journal of Pathology 146(5), 1029.
12. Cohen S, Carpenter G. 1975. Human epidermal growth factor: isolation and chemical and biological properties. Proceedings of the National Academy of Sciences 72(4), 1317-1321.
13. Norouzi M, Boroujeni SM, Omidvarkordshouli N, Soleimani M. 2015. Advances in skin regeneration: application of electrospun scaffolds. Advanced Healthcare Materials 4(8), 1114-1133.
14. Garcia-Orue I, Gainza G, Gutierrez FB, Aguirre JJ, Evora C, Pedraz JL, Hernandez RM, Delgado A, Igartua M. 2017. Novel nanofibrous dressings containing rhEGF and Aloe vera for wound healing applications. International Journal of Pharmaceutics 523(2), 556-566.
15. Wang Z, Qian Y, Li L, Pan L, Njunge LW, Dong L, Yang L. 2016. Evaluation of emulsion electrospun polycaprolactone/hyaluronan/ epidermal growth factor nanofibrous scaffolds for wound healing. Journal of Biomaterials Applications 30(6), 686-698.
16. Jang, C. S., Kim, S. I., Kim, H. K., Kweon, C. O., Kim, B. W., Kim, D. C., ... & Ryu, J. K. (2014). Plateletpheresis: The process, devices, and indicators of product quality. Journal of Life Science 24(9), 1030-1038.
17. McCullough, J. 2010. Overview of platelet transfusion. In Seminars in Hematology 47(3), 235-242. WB Saunders.
18. El-Timamy A, El Sharaby F, Eid F, El Dakroury A, Mostafa Y, Shaker O. 2020. Effect of platelet-rich plasma on the rate of orthodontic tooth movement: a split-mouth randomized trial. The Angle Orthodontist 90(3), 354-361.
19. Li J, Chen M, Wei X, Hao Y, Wang J. 2017. Evaluation of 3D-printed polycaprolactone scaffolds coated with freeze-dried platelet-rich plasma for bone regeneration. Materials 10(7), 831.
20. Zhang X, Yao D, Zhao W, Zhang R, Yu B, Ma G, Li Y, Hao D, Xu FJ. 2021. Engineering Platelet‐Rich Plasma Based Dual‐Network Hydrogel as a Bioactive Wound Dressing with Potential Clinical Translational Value. Advanced Functional Materials 31(8), 2009258.
21. Farzamfar S, Esmailpour F, Rahmati M, Vaez A, Mirzaii M, Garmabi B, Shayannia A, Ebrahimi E, Vahedi H, Salehi M. 2017. Poly-lactic acid/gelatin nanofiber (PLA/GTNF) conduits containing platelet-rich plasma for peripheral nerve regeneration. International Journal of Health Studies 3(2), 18.
22. Miroshnichenko S, Timofeeva V, Permyakova E, Ershov S, Kiryukhantsev-Korneev P, Dvořaková E, Shtansky DV, Zajíčková L, Solovieva A, Manakhov A. 2019. Plasma-coated polycaprolactone nanofibers with covalently bonded platelet-rich plasma enhance adhesion and growth of human fibroblasts. Nanomaterials 9(4), 637.
23. Unnithan AR, Barakat NA, Pichiah PT, Gnanasekaran G, Nirmala R, Cha YS, Jung CH, El-Newehy M, Kim HY. 2012. Wound-dressing materials with antibacterial activity from electrospun polyurethane–dextran nanofiber mats containing ciprofloxacin HCl. Carbohydrate Polymers 90(4), 1786-1793.
24. Alavarse AC, de Oliveira Silva FW, Colque JT, da Silva VM, Prieto T, Venancio EC, Bonvent JJ. 2017. Tetracycline hydrochloride-loaded electrospun nanofibers mats based on PVA and chitosan for wound dressing. Materials Science and Engineering: C 77, 271-281.
25. Choi JI, Kim MS, Chung GY, Shin HS. 2017. Spirulina extract-impregnated alginate-PCL nanofiber wound dressing for skin regeneration. Biotechnology and Bioprocess Engineering 22, 679-685.
26. Hsieh YL. 2000. Mat Characteristics of Polyester Fibers. Pastore CM, Kiekens P (Ed), Mat Characteristics of Fibers and Textiles. New York: Markel Dekker Inc. p.33-57.
27. Cordenonsi LM, Faccendini A, Rossi S, Bonferoni MC, Malavasi L, Raffin R, ... & Ferrari F. 2019. Platelet lysate loaded electrospun scaffolds: Effect of nanofiber types on wound healing. European Journal of Pharmaceutics and Biopharmaceutics, 142, 247-257.
28. Karuppannan SK, Dowlath MJ, Ramalingam R, Musthafa SA, Ganesh MR, Chithra V, Ravindran B, Arunachalam KD. 2022. Quercetin functionalized hybrid electrospun nanofibers for wound dressing application. Materials Science and Engineering: B 285, 115933.
29. Tanha S, Rafiee‐Tehrani M, Abdollahi M, Vakilian S, Esmaili Z, Naraghi ZS, Seyedjafari E, Javar HA. 2017. G‐CSF loaded nanofiber/nanoparticle composite coated with collagen promotes wound healing in vivo. Journal of Biomedical Materials Research Part A 105(10), 2830-2842.
30. Kim SE, Heo DN, Lee JB, Kim JR, Park SH, Jeon SH, Kwon IK. 2009. Electrospun gelatin/polyurethane blended nanofibers for wound healing. Biomedical Materials 4(4), 044106.
31. Yang Y, Hu H. 2017. Spacer fabric-based exuding wound dressing–Part II: Comparison with commercial wound dressings. Textile Research Journal 87(12), 1481-1493.
32. Zhang C, Yuan X, Wu L, Han Y, Sheng J. 2005. Study on morphology of electrospun poly (vinyl alcohol) mats. European Polymer Journal 41(3), 423-432.
33. Hashmi M, Ullah S, Ullah A, Khan MQ, Hussain N, Khatri M, Bie X, Lee J, Kim IS. 2020. An optimistic approach “from hydrophobic to super hydrophilic nanofibers” for enhanced absorption properties. Polymer Testing 90, 106683.
34. Zhao JH, Xu L, Liu Q. 2015. Effect of ethanol post-treatment on the bubble-electrospun poly (vinyl alcohol) nanofiber. Thermal Science 19(4), 1353-1356.
35. Selçuk E, Calapoğlu NŞ. 2022. Overview of primary messengers and their receptors. Suleyman Demirel University Journal of Health Sciences 13(3), 559-566.
36. Fredenberg S, Wahlgren M, Reslow M, Axelsson A. 2011. The mechanisms of drug release in poly (lactic-co-glycolic acid)-based drug delivery systems--a review. International Journal of Pharmaceutics 415(1-2), 34–52.
37. Shah SS, Cha Y, Pitt CG. 1992. Poly (glycolic acid-co-dl-lactic acid): diffusion or degradation-controlled drug delivery? Journal of Controlled Release 18(3), 261-270.
38. Kajdič S, Planinšek O, Gašperlin M, Kocbek P. 2019. Electrospun nanofibers for customized drug-delivery systems. Journal of Drug Delivery Science and Technology 51, 672-681.
39. Laha A, Sharma CS, Majumdar S. 2017. Sustained drug release from multi-layered sequentially crosslinked electrospun gelatin nanofiber mesh. Materials Science and Engineering: C 76, 782-786.
40. Li T, Ding X, Tian L, Hu J, Yang X, Ramakrishna S. 2017. The control of beads diameter of bead-on-string electrospun nanofibers and the corresponding release behaviors of embedded drugs. Materials Science and Engineering: C 74, 471-477.
41. Ceylan M, Yang SY, Asmatulu R. 2017. Effects of gentamicin-loaded PCL nanofibers on growth of Gram positive and Gram-negative bacteria. International Journal of Applied Microbiology and Biotechnology Research 5, 40-51.
42. Arnoczky SP, Shebani-Rad S. 2013. The basic science of platelet-rich plasma (PRP): What clinicians need to know? Sports Medicine and Arthroscopy Review 21(4), 180-185.
43. Wang Q, Qian Z, Liu B, Liu J, Zhang L, Xu J. 2019. In vitro and in vivo evaluation of new PRP antibacterial moisturizing dressings for infectious wound repair. Journal of Biomaterials Science, Polymer Edition 30(6), 462-485.
44. Cheng H, Yang X, Che X, Yang M, Zhai G. 2018. Biomedical application and controlled drug release of electrospun fibrous materials. Materials Science and Engineering:C 90, 750-763.
45. Diaz-Gomez L, Alvarez-Lorenzo C, Concheiro A, Silva M, Dominguez F, Sheikh FA, Cantu T, Desai R, Garcia VL, Macossay J. 2014. Biodegradable electrospun nanofibers coated with platelet-rich plasma for cell adhesion and proliferation. Materials Science and Engineering:C 40, 180-188.
46. Üstündağ GC, Karaca E, Özbek SE, Çavuşoğlu İ. 2010. In vivo evaluation of electrospun poly (vinyl alcohol) /sodium alginate nanofibrous mat as wound dressing. Tekstil ve Konfeksiyon 20(4), 290-298
Yılmaz, H., Aras, C., Karaçay, M., Altuntuğ Cesur, M. İ., et al. (2024). Investigation of the Release of Growth Factors from Apheresis Platelet Concentrate (APC) Loaded Three Layered Composite Nanofiber Surface. Textile and Apparel, 34(3), 244-252. https://doi.org/10.32710/tekstilvekonfeksiyon.1309432
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