Bitki patojeni funguslara karşı Grindelia robusta Nutt., Tanacetum praeteritum subsp. praeteritum ve Alchemilla vulgaris L. ekstraktlarının antifungal aktivitelerinin değerlendirilmes

Year 2021, Volume: 2 Issue: 1, 57 - 63, 29.01.2021

Oğuz Özbek , Yakup Budak , Çiğdem Özyiğit , Yusuf Yanar

Abstract

Evaluation of antifungal activities of Grindelia robusta Nutt., Tanacetum praeteritum subsp. praeteritum and Alchemilla vulgaris L. extracts against plant pathogenic fungi

Abstract

Purpose: Present study, we were carried out to evaluate the antifungal activities of extracts of Grindelia robusta Nutt., Tanacetum praeteritum subsp. praeteritum and Alchemilla vulgaris L. against Fusarium oxysporum f. sp. lycopersici, Botrytis cinerea Macrophamina phaseolina, Rhizoctania solani, Alternaria alternata, Cylindrocarpon destructans and Phytopthora infestans.
Material and Methods: The screening of the antifungal potential of three extract forms from three different plant species was performed in vitro by using Agar-diffusion method on Potato Dextrose Agar media (PDA). One doses (1.0%, 1.5% and 2,0%) of the three diffent extracts were tested. Experimental results were obtained in three (n=3) replicates.
Results: All plant extracts prepared in the study exhibited significant activity against plant pathogenic fungi. In particular, Tanacetum praeteritum subsp. praeteritum prevented the growth of micelles between 73.5 - 100% in five different fungal species, while Grindelia robusta Nutt. had a 100% effect on two of the four different fungus species.
Conclusion: The extracts exhibited an antifungal activity against all the tested fungi. The analysis shown that the all extracts caused significantly inhibited the mycelium growth of fungi. This study paves the way for the development of new methods for environmentally friendly and economically viable phytosanitary practices.

Keywords

Anti-fungal activity, plant extract, plant pathogens, fungicide

References

• Bagcı, E. (2009). Composition of the essential oils of Tanacetum densum (Lab.) Schultz Bip. subsp. amani and T. densum (Lab.) Schultz Bip. subsp. laxum (Asteraceae) from Turkey. Asian Journal of Chemistry, 21(8), 6547-6554.
• Boroja, T., Mihailović, V., Katanić, J., Pan, S. P., Nikles, S., Imbimbo, P. et al. (2018). The biological activities of roots and aerial parts of Alchemilla vulgaris L. South African Journal of Botany, 116, 175-184. https://doi.org/10.1016/j.sajb.2018.03.007
• Brown, A. M. G., Edwards, C. M., Davey, M. R., Power, J. B., Lowe, K. C. (1997). Effects of extracts of Tanacetum species on human polymorphonuclear leucocyte activity in vitro. Phytotherapy Research: An International Journal Devoted to Medical and Scientific Research on Plants and Plant Products, 11(7), 479-484. https://doi.org/10.1002/(SICI)1099-1573(199711)11:7<479::AID-PTR129>3.0.CO;2-W
• Copping, L. G., Menn, J. J. (2000). Biopesticides: a review of their action, applications and efficacy. Pest Management Science: Formerly Pesticide Science, 56(8), 651-676. https://doi.org/10.1002/1526-4998(200008)56:8<651::AID-PS201>3.0.CO;2-U
• Deans, S. G., Sobada, K. P. (1990). Antimicrobial Properties of Marjoram (Origanum marjorana L.) Volatile Oil, Flavour and Fragrance Journal, 5, 187-190. https://doi.org/10.1002/ffj.2730050311
• El-Shamy, A. M., El-Hawary, S. S., El-Shabrawy, A. O., El-Hefnawy, H. M., Glasl, H. (2000). Essential oil composition of three Grindelia species. Journal of essential oil research, 12(5), 631-634. https://doi.org/10.1080/10412905.2000.9712175
• Ferreres, F., Grosso, C., Gil-Izquierdo, A., Valentão, P., Azevedo, C., Andrade, P. B. (2014). HPLC-DAD-ESI/MSn analysis of phenolic compounds for quality control of Grindelia robusta Nutt. and bioactivities. Journal of Pharmaceutical and Biomedical Analysis, 94, 163-172. http://dx.doi.org/10.1016/j.jpba.2014.01.046
• Fraternale, D., Giamperi, L., Bucchini, A., Ricci, D. (2007). Essential oil composition and antioxidant activity of aerial parts of Grindelia robusta from Central Italy. Fitoterapia, 78(6), 443-445. https://dx.doi.org/10.1016/j.fitote.2007.04.011
• Gören, N. (1995). Sesquiterpene lactones from Tanacetum praeteritum. Phytochemistry, 38(5), 1261-1264. https://doi.org/10.1016/0031-9422(94)00746-G
• Gürdere, M. B., Aydın, A., Yencilek, B., Ertürk, F., Özbek, O., Erkan, S., Budak, Y., Ceylan, M. (2020). Synthesis, antiproliferative, cell cytotoxicity activity, DNA binding features and molecular d ocking study of novel enamine derivatives. Chemistry & Biodiversity, 17(7), e2000139. https://doi.org/10.1002/cbdv.202000139
• Iqbal, J., Yousaf, U., Zia, S., Asgher, A., Afzal, R., Ali, M., et al. (2019). Pulses Diseases “Important limiting factor in yield’’ and their Managements. Asian Journal of Research in Crop Science, 1-21. https://doi.org/10.9734/ajrcs/2019/v3i230045
• Jurić, T., Stanković, J. S. K., Rosić, G., Selaković, D., Joksimović, J., Mišić, D., et al. (2020). Protective effects of Alchemilla vulgaris L. extracts against cisplatin-induced toxicological alterations in rats. South African Journal of Botany, 128, 141-151. https://doi.org/10.1016/j.sajb.2019.09.010
• Krenn, L., Wollenweber, E., Steyrleuthner, K., Görick, C., Melzig, M. F. (2009). Contribution of methylated exudate flavonoids to the anti-inflammatory activity of Grindelia robusta. Fitoterapia, 80(5), 267-269. https://dx.doi.org/10.1016/j.fitote.2009.03.001
• Neagu, E., Paun, G., Albu, C., Radu, G. L. (2015). Assessment of acetylcholinesterase and tyrosinase inhibitory and antioxidant activity of Alchemilla vulgaris and Filipendula ulmaria extracts. Journal of the Taiwan Institute of Chemical Engineers, 52, 1-6. http://dx.doi.org/10.1016/j.jtice.2015.01.026
• Nowak, S., Rychlińska, I. (2012). Phenolic acids in the flowers and leaves of Grindelia robusta Nutt. and Grindelia squarrosa Dun.(Asteraceae). Acta poloniae pharmaceutica, 69(4), 693.
• Özbek, O., Gürdere, M. B. (2020). A review on the synthesis and applications of molecules as anticonvulsant drug agent candidates. Medicinal Chemistry Research, 29(9), 1553-1578. https://doi.org/10.1007/s00044-020- 02595-4
• Özbek, O., Usta, N. C., Gürdere, M. B., Aslan, O. N., Budak, Y., Ceylan, M. (2017). Synthesis and antibacterial screening of novel 2-(4-(aryl)thiazol-2-yl)-3a,4,7,7a-tetrahydro-1H-4,7-ethanoisoindole-1,3(2H)-dione derivatives. Phosphorus, Sulfur, and Silicon and the Related Elements, 192(10), 1153-1157. https://doi.org/10.1080/10426507.2017.1354209
• Özbek, O., Budak, Y., Berkel, C., Özyigit, C., Yanar, Y. (2020). The use of Marrubium vulgare L. plant extracts in the control of fungal plant pathogens. International Journal of Agriculture Environment and Food Sciences, 4(4), 476-482. https://doi.org/10.31015/jaefs.2020.4.11
• Tasić-Kostov, M., Arsić, I., Pavlović, D., Stojanović, S., Najman, S., Naumović, S., Tadić, V. (2019). Towards a modern approach to traditional use: in vitro and in vivo evaluation of Alchemilla vulgaris L. gel wound healing potential. Journal of ethnopharmacology, 238, 111789. https://doi.org/10.1016/j.jep.2019.03.016
• Zerbe, P., Rodriguez, S. M., Mafu, S., Chiang, A., Sandhu, H. K., O'Neil‐Johnson, M., et al. (2015). Exploring diterpene metabolism in non‐model species: transcriptome‐enabled discovery and functional characterization of labda‐7,13E‐dienyl diphosphate synthase from Grindelia robusta. The Plant Journal, 83(5), 783-793. https://doi.org/10.1111/tpj.12925