Atatürk Baraj Gölü’nde (Türkiye) Yaşayan Carassius gibelio (Bloch, 1782) türünün mtDNA COI ve cyt b Analizi

Abstract

Amaç: Bu çalışmanın amacı, Atatürk Baraj Gölü’nde yaşayan C. gibelio türünün mtDNA COI ve Cyt b gen bölgelerine ait sekanslara dayalı olarak gen bankasındaki sekanslarla karşılaştırılması ve filogenetik analizlerin yapılmasıdır.
Materyal ve Yöntem: Atatürk Baraj Gölü Bozova bölgesinde yaşayan hedef türe ait bireyler balıkçılardan rastgele seçilerek satın alınmıştır. Total DNA izolasyonu, GeneJET Genomic DNA Purification Kit (Thermo Scientific) kullanılarak kas dokusundan protokol talimatları doğrultusunda yapılmıştır. Daha sonra mtDNA COI ve Cyt-b bölgelerine ait özgül primerler kullanılarak ilgili bölgeler PCR yöntemi ile çoğaltılmıştır. Elde edilen PCR ürünleri Agaroz jelde yürütülmüş ve ürün oluşturan örnekler seçilerek hizmet alımı şeklinde ticari firmaya gönderilmiş ve 3500 XL Genetic Analyzer cihazı ile dizi analizi yaptırılmıştır.
Bulgular: mtDNA COI ve cyt b gen bölgeleri için sırayla 620 ve 580 bp uzunlukta sekanslar elde edilmiştir. mtDNA COI bölgesi için daha önce Türkiye’de yaşayan bireylerle aynı haplotipler tespit edilmesine rağmen mtDNA Cyt b gen bölgesi için daha önce tanımlanmayan yeni bir haplotip tespit edilmiştir.
Sonuç: Atatürk Baraj Gölü’nde yaşayan C. gibelio türüne ait bireylerin mtDNA COI ve Cyt b gen bölgelerine ait sekans analizleri ilk defa bu çalışmada yapılmış ve gen bankasındaki verilerle karşılaştırılmıştır.

Keywords

• Atatürk Baraj Gölü
• Carassius gibelio
• mtDNA
• COI
• cyt b

mtDNA COI and cyt b Analysis of Carassius gibelio (Bloch, 1782) Living in Atatürk Dam Lake (Turkey)

Abstract

Objective: The aim of this study is to compare the C. gibelio species living in Atatürk Dam Lake with the sequences in the gene bank based on the sequences of the mtDNA COI and Cyt b gene regions and to perform phylogenetic analyzes.
Material and Methods: Individuals of the target species living in the Bozova region of Atatürk Dam Lake were randomly selected from fishermen and purchased. Total DNA isolation was performed from muscle tissue using the GeneJET Genomic DNA Purification Kit (Thermo Scientific) according to the protocol instructions. Then, using specific primers of mtDNA COI and Cyt-b regions, the relevant regions were amplified by PCR method. The obtained PCR products were carried out in Agarose gel and the samples forming the product were selected and sent to the commercial firm as service procurement and sequence analysis was performed with the 3500 XL Genetic Analyzer device.
Results: Sequences of 620 and 580 bp in length were obtained for the mtDNA COI and cyt b gene regions, respectively. Although the same haplotype was detected for the mtDNA COI region with individuals living in Turkey before, a new haplotype was detected for the mtDNA cyt b gene region that was not previously defined.
Conclusion: Sequence analyzes of mtDNA COI and cyt b gene regions of individuals belonging to the C. gibelio species living in Atatürk Dam Lake were performed for the first time in this study and compared with the data in the gene bank.

Keywords

• Atatürk Dam Lake
• Carassius gibelio
• mtDNA
• COI
• cyt b

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