AGRONOMIC CHARACTERIZATION OF AN EMS-MUTAGENIZED POPULATION FOR SELECTING HIGH-YIELDING AND GLUTENENRICHED INDUSTRIAL WHEAT

Abstract

A mutant population was generated using a popular wheat cultivar of Bangladesh; BARI GOM-28, with ethyl methanesulfonate (EMS) to create diversity in terms of different agronomic characteristics and grain quality parameters. An EMS concentration ranging from 0.2% to 1.2% was used and the optimum was found to be 0.8%. This study was initiated with 16,000 seeds, where 1,581 lines survived under greenhouse conditions up to M4 generation. Through 3 subsequent field trials, 3 promising lines, namely, 0037/17, 0020/17 and 0023/17 were selected with enhanced spike length, number of spikes per plant, number of spikelets per spike, number of grains per spike, grains weight per plant and 1000-grain weight. Such improved agronomic traits contributed towards a greater yield potential of 0037/17 (5.94-6.10 t ha-1), 0020/17 (5.47-5.54 t ha-1) and 0023/17 (4.97-5.20 t ha-1) than BARI GOM-28 (3.63-3.69 t ha-1) in the multi-location trial. Improvement in certain bread-making qualities like wet gluten content (>28%) and total protein content (~13%) was also observed and compared to BARI GOM-28 which had 22% wet gluten and 11% total protein. Therefore, these mutant lines could be used as a valuable resource for genetic studies to dissect the function of the genes controlling such desired parameters as well as superior breeding lines.

Keywords

• Gluten
• Mutation Breeding
• Mutant population
• Triticum aestivum L.
• EMS

References

1. Aisawi, K.A.B., M.P. Reynolds, R.P. Singh and M.J. Foulkes. 2015. The Physiological Basis of the Genetic Progress in Yield Potential of CIMMYT Spring Wheat Cultivars from 1966 to 2009. Crop Sci. 55(4):1749-1764.
2. Azad, A.K., M.A. Wahab, M.G. Shaha, J. Nesa, M.L. Rahman, M.H.H. Rahman and M. Al-Amin. 2019. Krishi Prajukti Hatboi. Bangladesh Agricultural Research Institute, Gazipur-1701, Bangladesh.
3. Barma, N.C.D., A. Hossain, M.A. Hakim,K.A. Mottaleb, M.A. Alam, M.M.A. Reza and M.M. Rohman. 2019. Progress and Challenges of Wheat Production in the Era of Climate Change: A Bangladesh Perspective. In: Hasanuzzaman M, Nahar K, Hossain MA (eds) Wheat Production in Changing Environments: Responses, Adaptation and Tolerance. Springer Singapore. pp. 615-679.
4. Bojnanska. T. and H. Francakova. 2002. The use of spelt wheat (Triticum spelta L.) for baking applications. Rostl Vyroba. 48(4):141-147.
5. Carson. G.R. and N.M. Edwards. 2009. Criteria of Wheat and Flour Quality. In: Khan K, Shewry PR (eds) Wheat (Fourth Edition). AACC International Press. pp. 97-118.
6. Chen. L., L. Huang., D. Min., A. Phillips., S. Wang., P.J. Madgwick, M.A.J. Parry and Y.G. Hu. 2012. Development and Characterization of a New TILLING Population of Common Bread Wheat (Triticum aestivum L.). PLoS One.7(7): e41570.
7. Clare. M.E.N., N. Wellner., L.A. Salt., J. Robertson and J.A. Jenkins 2012. Wheat proteins and bread quality. In: Cauvain SP (ed) Breadmaking (Second Edition). Woodhead Publishing. pp. 100-122.
8. Doyle. J.J. and J.L. Doyle 1990. Isolation of plant DNA from fresh tissue. Focus. 12: 13-15.

9. FAOSTAT, 2020. Countries by Commodities. http://www.fao.org/faostat/en/#rankings/countries_by_commodity_imports. (Accessed August 15, 2022).
10. Flohr B.M., J.R. Hunt, J.A. Kirkegaard, J.R. Evans, A. Swan and B. Rheinheimer. 2018. Genetic gains in NSW wheat cultivars from 1901 to 2014 as revealed from synchronous flowering during the optimum period. Eur J Agron. 98: 1-13.
11. Gaju O., M.P. Reynolds, D.L. Sparkes and M.J. Foulkes. 2009. Relationships between Large-Spike Phenotype, Grain Number, and Yield Potential in Spring Wheat. Crop sci. 49 (3): 961-973.
12. Jankowicz-Cieslak J., C. Mba, B.J. Till. 2017. Mutagenesis for Crop Breeding and Functional Genomics. In: Jankowicz-Cieslak J, Tai TH, Kumlehn J, Till BJ (eds) Biotechnologies for Plant Mutation Breeding: Protocols. Springer International Publishing, Cham. pp. 3-18.
13. Karim M., M. Awal and M. Akter. 2010. Forecasting of Wheat Production in Bangladesh. Bangladesh J Agr Res 35: 17-28.
14. Kong W., L. Wang, P. Cao, X. Li, J. Ji, P. Dong, X. Yan, C. Wang, H. Wang and J. Sun. 2020. Identification and genetic analysis of EMS-mutagenized wheat mutants conferring lesion-mimic premature aging. BMC Genet. 21 (1): 88.
15. Kumar R., V. Singh, S.K. Pawar, P.K. Singh, A. Kaur and D. Sharma. 2019. Abiotic Stress and Wheat Grain Quality: A Comprehensive Review. In: Hasanuzzaman M, Nahar K, Hossain MA (eds) Wheat Production in Changing Environments: Responses, Adaptation and Tolerance. Springer Singapore, Singapore. pp. 63-87.
16. Lethin J., S.S.M. Shakil, S. Hassan, N. Sirijovski, M. Töpel, O. Olsson and H. Aronsson. 2020. Development and characterization of an EMS-mutagenized wheat population and identification of salt-tolerant wheat lines. BMC Plant Biol. 20 (1):18.
17. Mughal, I., Y. Shah, S. Tahir, W. Haider, M. Fayyaz, T. Yasmin, M. Ilyas and S. Farrakh. 2020. Protein quantification and enzyme activity estimation of Pakistani wheat landraces. PLoS One 15 (9): e0239375.
18. Naruoka, Y., L.E. Talbert, S.P. Lanning, N.K. Blake, J.M. Martin and J.D. Sherman. 2011. Identification of quantitative trait loci for productive tiller number and its relationship to agronomic traits in spring wheat. Theor. Appl. Genet. 123 (6):1043.
19. OECD. 2021. Wheat in Bangladesh, https://oec.world/en/profile/bilateral-product/wheat/reporter/bgd#:~:text=About&text=Exports%20In%202020%2C%20Bangladesh%20exported,)%20and%20Singapore%20(%24143). (Accessed by August 15, 2022).
20. Oikonomou, N.A., S. Bakalis, M.S. Rahman and M.K. Krokida. 2015. Gluten Index for Wheat Products: Main Variables in Affecting the Value and Nonlinear Regression Model. Int. J. Food Prop. 18(1): 1-11.
21. Oladosu, Y., M.Y. Rafii, N. Abdullah, G. Hussin, A. Ramli, H.A. Rahim, G. Miah and M. Usman. 2016. Principle and application of plant mutagenesis in crop improvement: a review. Biotechnol. Equip. 30 (1): 1-16.
22. Roychowdhury, R. and J. Tah. 2013. Mutagenesis—A Potential Approach for Crop Improvement. In: Hakeem KR, Ahmad P, Ozturk M (eds) Crop Improvement: New Approaches and Modern Techniques. Springer US, Boston, MA, pp 149-187.
23. Sakin, M.A., A. Yildirim and S. Gökmen. 2005. Determining some yield and quality characteristics of mutants induced from a durum wheat (Triticum durum Desf.) cultivar. Turk J Agric For. 29(1): 61-67.
24. Shirazy, B., A. Islam, M. Dewan and S. Shahidullah. 2017. Crops and Cropping Systems in Dinajpur Region. Bangladesh Rice J. 21(2): 143-156.
25. Slade, A.J., S.I. Fuerstenberg, D. Loeffler, M.N. Steine and D. Facciotti. 2005. A reverse genetic, nontransgenic approach to wheat crop improvement by TILLING. Nat Biotechnol. 23 (1): 75-81.
26. Tonmoy, W. 2022. Wheat consumption to rise 6pc: USDA, https://www.thedailystar.net/business/economy/news/wheat-consumption-rise-6pc-usda-2955416. (Accessed August 15, 2022).
27. Uthayakumaran, S. and C. Wrigley. 2017. Chapter 5 - Wheat: Grain-Quality Characteristics and Management of Quality Requirements. In: Wrigley C, Batey I, Miskelly D (eds) Cereal Grains (Second Edition). Woodhead Publishing, pp 91-134.
28. Venske, E., R.S. dos Santos, C. Busanello, P. Gustafson and A. Costa de Oliveira. 2019. Bread wheat: a role model for plant domestication and breeding. Hereditas. 156(1): 16.
29. Wang, R., Y. Liu, K. Isham, W. Zhao, J. Wheeler, N. Klassen, Y. Hu, J.M. Bonman and J. Chen. 2018. QTL identification and KASP marker development for productive tiller and fertile spikelet numbers in two high-yielding hard white spring wheat cultivars. Mol Breed. 38(11): 135.
30. Yamagata, H., T. Tanisaka and Y. Okumoto. 1989. Induction of Extremely-early-heading Mutants in Wheat : Studies on the Utility of Artificial Mutations in Plant Breeding XVII. Jpn J Breed. 39(1): 89-99.