Determination of Combining Ability in Ethiopian Mustard (Brassica carinata) Parents According to Line x Tester Analysis

Year 2025, Volume: 30 Issue: 2, 257 - 265, 28.12.2025

Cansu Dolgun , Mehmet Sincik

https://doi.org/10.17557/tjfc.1714416

Abstract

This research was conducted to determine the parents and hybrids with superior general and specific combining ability in 20 F1 hybrids including 5 female lines and 4 male testers. In study, 5 advanced lines were used as female parents and 4 registered varieties were used as male parents. The nine parents were crossed to procedure 20 F1 hybrids according to line x tester mating design during 2021-2022 growing season. The 20 crosses and 9 parents were evaluated in randomized complete block design with three replications during 2022-2023 growing season. According to results, it was determined that BC10 and BC22 lines in seed yield, Awassa tester in pod number per plant and seed yield had given the highest general combining ability. However, BC10 x Saryan, BC13 x Awassa and BC21 x Awassa hybrid combinations showed the highest specific combining ability in seed yield. It was stated that both additive and non-additive gene effects were efficacious for yield and some important yield components in the hybrid population studied. The promising hybrid combinations BC10 x Saryan (48.72**), BC21 x Awassa (126.74**) and BC13 x Awassa (127.83**) possessed positive significant heterosis for seed yield.

Keywords

General combining ability , Heterosis , Mustard , Specific combining ability , Yield components

References

• Ahmad, H.B., Rauf, S., Chattha, W.S., Khaliq, A., Amin, A., & Ali, S. (2022). Genetic analysis of various traits in oilseed Brassicas. Indian Journal of Agricultural Research, 56(2), 115–121. DOI:10.18805/IJARe.A-625
• Aksoy, E., Özsoy, G., Dirim, M.S., & Tümsavaş. Z. (2001). Recent developments in remote sensing and GIS techniques in land use mapping: U.U. Dönücü area example. GAP II. Agriculture Congress (pp.1045-1052). Şanlıurfa, Türkiye. (In Turkish)
• Bozzini, A., Calcagno, F., & Soare, T. (2007). “Sincron”, a new Brassica carinata cultivar for biodiesel production. Helia, 46, 207-214. DOI:10.2298/HEL0746207B
• Bouaid, A., Diaz, Y., Martinez, M., & Aracil, J. (2005). Pilot plant studies of biodiesel production using Brassica carinata as raw material. Catalysis Today, 106(1-4), 193-196. DOI:10.1016/j.cattod.2005.07.163
• Cardone, M., Mazzoncini, M., Menini, S., Rocco, V., Senatore, A., Seggiani, M., & Vitolo, S. (2003). Brassica carinata as an alternative oil crop for the production of biodiesel in Italy: Agronomic evaluation, fuel production by transesterification and characterization. Biomass and Bioenergy, 25(6), 623-636. DOI:10.1016/S0961-9534(03)00058-8.
• Ceyhan, E., & Avcı, M.A. (2005). Combining ability and heterosis for grain yield and some yield components in pea (Pisum sativumL.). Pakistan Journal of Biological Sciences, 8(10),1447-1452. DOI: 10.3923/pjbs.2005.1447.1452
• Ceyhan, E., Avci, M.A., & Karadaş, S. (2008). Line x tester analysis in pea (Pisum sativum L.): Identification of superior parents for seed yield and its components. African Journal of Biotechnology, 7(16), 2810-2817.
• Ceyhan, E., Kahraman, A., Avcı M.A., & Dalgıç, H. (2014a). Combining ability of bean genotypes estimated by line x tester analysis under highly-calcareous soils. The Journal of Animal & Plant Sciences, 24(29), 579-584.
• Ceyhan, E., Harmankaya, M., & Kahraman, A. (2014b). Combining ability and heterosis for concentration of mineral elements and protein in common bean (Phaseolus vulgaris L.). Turkish Journal of Agriculture and Forestry, 38(5), 581-590. DOI:10.3906/tar-1307-56
• Ceyhan, E., & Şimşek, D. (2021). Determination of some agricultural characters and their heredity through line x tester method in dry bean (Phaseolus vulgaris L.). Atatürk University Journal of Agricultural Faculty, 52 (2), 148-159. https://doi.org/10.30910/turkjans.680042
• Chaudhari, V.A., Kamdi, S.R., Shinde, J., Baviskar, S.B., Meshram, M.P., & Tajne, D. (2022). Combining ability studies in different species of mustard. The Pharma Innovation Journal, 11(12), 3324-3328.
• Dholu, V.K., Sasidharan, N., Suthar, K., Bhusan, B., & Patel, J.N. (2014). Heterosis and combining ability analysis in Indian mustard [B. juncea (L.) Czern & Coss.]. Intl. J. Agril. Sci., 10, 102-107.
• Gan, Y., Malhi, S.S., Brandt, S.A., & McDonald, C.L. (2008). Assessment of seed shattering resistance and yield loss in five oilseed crops. Can. J. Plant. Sci., 88, 267–270. https://doi.org/10.4141/CJPS07028
• Istipliler D., Ilker, E., Tonk, F.A., Gizem, C.I., & Tosun, M. (2015). Line × tester analysis and estimating combining abilities for yield and some yield components in bread wheat. Turk. J. Field Crops, 20(1), 72-77. DOI:10.17557/.57234
• Kapadia, V.N., Sasidharan, N., Kalyanrao, D., & Kalola, A.D. (2020). Estimation of heterosis for yield related traits for single cross hybrids of Brassica species. Journal of Pharmacognosy and Phytochemistry, 9(1), 1766-1773.
• Kempthorne, O. (1957). An introduction to genetic statistics. John Wiley and Sons Inc., Newyork.
• Kose, A. (2017). Gene action and combining abitiy in line x tester population of safflower (Carthamus tinctorius L.). Turkish Journal of Field Crops, 22 (2), 197-203.
• Licata, M., La Bella, S., Leto, C., Bonsangue, G., Gennaro, M.C., Tuttolomondo, T., & Mario. L., (2017). Agronomic evaluation of Ethiopian mustard (Brassica carinata A. Braun) germplasm and physical-energy characterization of crop residues in a semi-arid area of Sicily (Italy). Chem. Eng. Trans., 58, 535–540. https://doi.org/10.3390/su12187492
• Meena, H.S., Kumar, A., Ram, B., Singh, V.V., Meena, P.D., Singh, B.K., & Singh, D. (2015). Combining ability and heterosis for seed yield and its components in Indian mustard (Brassica juncea L.). J. Agr. Sci. Tech., 17, 1861-1871.
• Sincik, M., Goksoy, A.T., & Turan, Z.M. (2011). The heterosis and combining ability of diallel crosses of rapeseed inbred lines. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 39(2), 242-248. https://doi.org/10.15835/nbha3926375
• Mulvaney, M.J., Leon, R.G., Seepaul, R., Wright, D.L., & Hoffman, T.L. (2019). Brassica carinata seeding rate and row spacing effects on morphology, yield, and oil. Agronomy Journal, 111(2), 528-535. DOI:10.2134/agronj2018.05.0316
• Nassimi, A.H., Raziuddin, A.S., Hassan, G., & Ali, N. (2006). Combining ability analysis for maturity and other traits in rapeseed (Brassica napus L.). Journal of Agronomy, 5(3), 523-526. https://doi.org/10.3923/JA.2006.523.526
• Ozcan, K., & Acikgoz, N. (1999). Development of a statistical package program for population genetics. In: 3rd Symposium on Computer Applications in Agriculture, Adana, Turkey.
• Rai, S.K., & Verma, A. (2005). Heterosis study in Indian mustard (B. juncea). Indian J. Genet., 65 (3), 217-18. Rakow, G. (1995). Developments in the breeding of edible oil in other Brassica species. In: Proceedings of the 9th Int. Rapeseed Conference (pp. 410-412), Cambridge, Henry Ling Ltd. Dochester, UK.
• Rameeh, V. (2012). Combining ability analysis of plant height and yield components in spring type of rapeseed varieties (Brassica napus L.) using line × tester analysis. Int. J. Agri. Forest., 2(1), 58-62. https://doi.org/10.29329/ijiaar.2019.217.15
• Rashid, M., Cheema, A.A., & Ashraf, M. (2007). Line x tester analysis in Basmati rice. Pakistan Journal of Botany, 39(6), 2035-2042.
• Sincik, M., Goksoy, A.T., & Turan, Z.M. (2011). The heterosis and combining ability of diallel crosses of rapeseed inbred lines. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 39(2), 242-248. DOI:10.15835/nbha3926375
• Sincik M., Sözen, E., Falk, C.F.K., Göksoy, A.T., & Açıkgöz, E. (2014). Heterosis and combining ability in a diallel cross of turnip rape genotypes. Turkish Journal of Field Crops, 19(2), 219-225. DOI:10.17557/tjfc.27610
• Singh, K.B., & Chaudhary, B.D. (1977). Biometrical methods in quantitative genetic analysis. Kalyani Publishers, New Delhi, India. DOI: 10.4236/ajps.2014.59125
• Singh, M., Srivastava, R.L., Prasad, L., & Dixit, R.K. (2003). Studies on heritability and genetic advance in Indian mustard (B. juncea). Advances in Plant Science. 16 (1), 263-66.
• Singh, A.K., Singh, B., & Sachan, J.N. (2005). Choice of parent and combining ability for yield and yield component characters in Indian mustard [Brassica juncea (L.) Czern and Coss.]. Agric. Sci. Digest, 25(2), 90-94.
• Singh, K.H., Singh, L., Parmar, N., Kumar, S., Nanjundan, J., & Singh, G. (2022). Molecular characterization and genetic diversity analysis in Indian mustard (Brassica juncea L. Czern & Coss.) varieties using SSR markers. Plos One, 17(8), e0272914. https://doi.org/10.1371/journal.pone.0272914
• Sprague G.F., & Tatum, L.A. (1942). General vs. specific combining ability in single crosses of corn. J. Am. Soc. Agron., 34, 923–932. https://doi.org/10.2134/agronj1942.00021962003400100008x
• Steel, R.G.D., & Torrie, J.H. (1980). Principles and procedures of statistics. A biometrical approach. Mc Grow-Hill Book Co. New York. DOI: 10.4236/jmp.2016.79090
• Surin, S., Kumar, A., Kumari, S., Kumar, Y., Tuti, A., & Suman, A. (2018). Heterosis for yield and its component in Indian mustard (Brassica juncea (L.) Czern & Coss). International Journal of Current Microbiology and Applied Science, 7, 3866–3871.
• Synrem, G.J., Rangare, N.R., Ibadaiahun, M., Bhusal, T.N., & Bahadure, D.M. (2015). Estimation of heterosis for seed yield and yield attributing traits in Indian mustard. Electr. J. Plant Breed., 6(1), 274-281.
• Tomar, A., Singh, M., & Tiwari, L.P. (2018). Combining ability (GCA & SCA), heterosis and inbreeding depression analysis for quantitative traits in yellow sarson (Brassica rapa var. yellow sarson). Journal of Pharmacognosy and Phytochemistry, 7(3), 2165-2170. DOI: https://doi.org/10.33545/2664844X
• Verma, O.P., Singh, S., Pradhan, S., Kar, G., & Rautaray, S.K. (2018). Irrigation, nitrogen and sulphur fertilization response on productivity, water use efficiency and quality of Ethiopian mustard (Brassica carinata) in a semi-arid environment. Journal of Applied and Natural Science, 10(2), 593-600. DOI: https://doi.org/10.31018/jans.v10i2.1741
• Verocai M., Gonz´alez-Barrios, P., & Mazzilli, S.R. (2024). A comparative study of yield components and their trade-off in oilseed crops (Brassica napus L. and Brassica carinata A. Braun). European Journal of Agronomy, 161, 127377. DOI:10.1016/j.eja.2024.127377
• Yadava, D.K., Singh, N., Vasudev, S., Singh, R., Singh, S., Giri, S.C., Dwivedi, V.K., & Prabhu, K.V. (2012). Combining ability and heterobeltiosis for yield and yield-contributing traits in Indian mustard (Brassica juncea). Indian J. Agri. Sci., 82(7), 563–567. DOI:10.56093/ijas.v82i7.21632
• Zanetti, F., Vamerali, T., & Mosca, G. (2009). Yield and oil variability in modern varieties of high-erucic winter oilseed rape (Brassica napus L, var, oleifera) and Ethiopian mustard (Brassica carinata A, Braun) under reduced agricultural inputs. Industrial Crops and Products, 30(2), 265-270. DOI:10.1016/j.indcrop.2009.05.002