IDENTIFICATION OF NEW QTL CONFERRING RESISTANCE TO ANTHRACNOSE (ASCOCHYTA RABIEI) IN A RIL POPULATION OF CHICKPEA

Year 2018, , 93 - 99, 15.12.2018

Arzu Yıldırım Abdullah Kahraman , Frederick John Muehlbauer Bahattin Tanyolac

https://doi.org/10.17557/tjfc.467465

Abstract

Ascochyta blight (AB) is a consistent problem affecting large growing areas of chickpea in all countries where
this crop is cultivated. This disease is capable of causing large yield losses under conducive environmental
conditions. To characterize the genetics of resistance to AB in chickpea, a population consisting of 77
recombinant inbred lines (RILs) derived from an inter-specific cross of Cicer arietinum (FLIP84-92C, resistant
parent) x Cicer reticulatum Lad. (PI 599072, susceptible parent) was used. Each RIL and the parents were
inoculated with blight spores by spraying. The RILs were scored for disease reactions under greenhouse
conditions at 20 °C in a 12 h photoperiod. A linkage map was constructed using RAPD markers. Eleven
linkage groups were obtained, of which three were small. The map spanned 889.1 cM with an average marker
density of 10.1 cM. Two QTL were detected on linkage groups 1 and 4, which together explained 31% of the
total phenotypic variation for AB resistance. These markers can improve precision of molecular breeding in
this population. 

Keywords

Ascochyta blight, chickpea, linkage map, QTL analysis

References

• Anbessa Y., B. Taran, T. D. Warkentin, A. Tullu, A. Vandenberg. 2009. Genetic analyses and conservation of QTL for ascochyta blight resistance in chickpea (Cicer arietinum L.) Theo Appl Genet. 119: 757–765.
• Arumuganathan, K. and E. D. Earle. 1991. Nuclear DNA content of some important plant species. lant Mol Biol Rep. 9: 208– 218.
• Barve, M. P., T. Arie, S. S. Salimath, F. J. Muehlbauer, T. L. Peever. 2003. Cloning and characterization of the mating type (MAT) locus from Ascochyta rabiei (Teleomorph: Didymella rabiei) and a MAT phylogeny of legumeassociated Ascochyta ssp. Fungal Genetics and Biology 39: 151-167.
• Bayraktar, H., F. S. Dolar, S. Maden. 2007. Mating type groups of Ascochyta rabiei (Teleomorph: Didymella rabiei), the causal agent of chickpea blight in central Anatolia. Turk. J. Agric. Forestry. 31: 41-46.
• Cho, S. and F. J. Muehlbauer. 2004. Genetic effect of differentially regulated fungal response genes on resistance to necrotropic fungal pathogens in chickpea (Cicer arietinum L.). Physiological and Molecular Plant Pathology 64: 57-66.
• Chowdhury M. A, C. P. Andrahennadi, A. E. Slinkard, A. Vandeberg. 2001. RAPD and SCAR markers for resistance to ascochyta blight in lentil. Euphytica 118:331–337.
• Cobos, M. J., J. Rubio, R. N. Strange, M. T. Moreno, J. Gil, T. Millan. 2006. A new QTL for ascochyta blight resistance in an RIL population derived from an inter specific cross in chickpea. Euphytica 149: 105-111.
• Collard, B. C. Y., P. K. Ades, E. C. K. Pang, J. B. Brouwer, P. W. J. Taylor. 2001. Prospecting for sources of resistance to ascochyta blight in wild Cicer species. Aust Plant Pathol. 30: 271–276.
• Dey, S. K. and G. Singh., 1993. Resistance to Ascochyta blight in chickpea – genetic basis. Euphytica 68: 147-153. Doyle J.J. and J.L. Doyle. 1990. Isolation of plant DNA from fresh tissue. Focus 12: 13–14.
• Eser, D. 1976. Heritability of some important plant characteristics, their relationship with plant yield and inheritance of Ascochyta blight resistance in chickpea (Cicer arietinum L.) Ankara University, Faculty of Agriculture press 620, Ankara, Turkey.
• Flandez-Galvez, H., P. K. Ades, R. Ford, E. C. K. Pang, P. W. J. Taylor. 2003. QTL analysis for Ascochyta blight resistance in an intra-specific population of chickpea (Cicer arietinum L.). Theo Appl Genet. 107: 1257-1265.
• Iruela, M., J. Rubio, F. Barro, J. I. Cubero, T. Millan, J. Gil. 2006. Detection of two quantitative trait loci for resistance to ascochyta blight in an intra-specific cross of chickpea (Cicer arietinum L.): Development of SCAR markers associated with resistance. Theo Appl Genet. 112: 278-287.
• Jamil, F. F., N. Sarwar, M. Sarwar, J. A. Khan, J. Geistlinger, G. Kahl. 2000. Genetic and pathogenic diversity within Ascochyta rabiei (Pass.) Lab populations in Pakistan causing blight of chickpea (Cicer arietinum L.). Physiological and Molecular Plant Pathology 57: 243-254.
• Kosambi, D. D. 1994.The estimation of map distances from recombination values. Eugene 12: 172-175.
• Kusmenoglu, I. 1990. Ascochyta blight of chickpea: Inheritance and relationship to seed size, morphological traits and isozyme variation. M.S. Thesis, Washington State Univ., Pullman.
• Lander, E. S., P. Green, J. Abrahamson, A. Barlow, M. J. Daly, S. E. Lincoln, L. Newburg. 1987. MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1: 174- 181.
• Millan, T., J. Rubio, M. Iruela, K. Daly, J. I. Cubero, J. Gil., 2003. Markers associated with ascochyta blight resistance in chickpea and their potential in marker-assisted selection. Field Crops Res. 84: 373–384.
• Morjane, H., J. Geistliner, M. Harrabi, K. Weising, G. Kahl. 1994. Oligonucleotide fingerprinting detects genetic diversity among Ascochyta rabiei from a single chickpea field in Tunisia. Curr Genet. 26: 191-197.
• Muehlbauer, F. J. and W. J. Kaiser. 1994.Using host plant resistance to manage biotic stress in cool season grain legumes. Euphytica 73: 1-10.
• Nelson, J. C. 1997. Qgene, Software for marker based genomic analysis and breeding. Mol. Breeding. 3: 239-245.
• Tewari, S.K. and M.P. Pandey. 1986. Genetics of resistance to ascochyta blight in chickpea (Cicer arietinum L.). Euphytica 35: 211-215.
• Ozkilinc, H., O. Frenkel, S. Abbo, R. Eshed, A. Sherman, D. Shtienberg, R. Ophir, C. Can. 2010. A comparative study of Turkish and Israeli populations of Didymella rabiei, the ascochyta blight pathogen of chickpea, Plant Pathology. 59: 492–503
• Paran I., D. Gidoni, R. Jacobsohn. 1997. Variation between and within broomrape (Orobanche) species revealed by RAPD markers. Heredity 78: 68–74.
• Rakshit, S., P. Winter, M. Tekeoglu, J. Juarez Munoz, T. Pfaff, A. M. Benko-Iseppon, F. J. Muehlbauer, G. Kahl. 2003. DAF marker tightly linked to a major locus for Ascochyta blight resistance in chickpea (Cicer arietinum L.). Euphytica 132: 23-30.
• Santra, D. K., M. Tekeoglu, M. Ratnaparkhe, W. J. Kaiser, F. J. Muehlbauer. 2000. Identification and mapping of QTLs conferring resistance to ascochyta blight in chickpea. Crop Sci. 40: 1606-1612.
• Singh, K. B. and M.V. Reddy. 1983. Inheritance of resistance to ascochyta blight in chickpea. Crop Science. 23: 9-10.
• Tanyolac B. 2003. Inter-simple sequence repeat (ISSR) and RAPD variation among wild barley (Hordeum. vulgare subsp. spontaneum) populations from west Turkey. Genetic Resources and Crop Evolution 50: 611–614.
• Tekeoglu, M., D. K. Santra, W. J. Kaiser, F. J. Muehlbauer. 2000. Ascochyta blight resistance inheritance in three chickpea recombinant inbred line population. Crop Sci. 40: 1251-1256.
• Tekeoglu, M., P. N. Rajesh, F. J. Muehlbauer. 2002. Integration of sequence tagged microsatellite sites to the chickpea genetic map. Theo Appl Genet 105: 847-854.
• Udupa, S. M. and M. Baum., 2003. Genetic dissection of pathotype specific resistance to ascochyta blight disease in chickpea (Cicer arietinum L.) using microsatellite markers. Theo Appl Genet. 106: 1196-1202.
• Vir, S., J. S. Grewal, V. P. Gupta. 1975. Inheritance of resistance to ascochyta blight in chickpea. Euphytica 24: 209-211.
• Winter, P., A M. Benko-Iseppon, B. Huttel, M. Ratnaparkhe, A. Tulu, G. Sonnante, T. Pfaff, M. Tekeoglu, D. Santra, V. J. Sant, P. N. Rajesh, G. Kahl, F. J. Muehlbauer. 2000. A linkage map of the chickpea (Cicer arietinum L.) genome based on recombinant inbred lines from a C. arietinum x C. reticulatum cross: localization of resistance genes for fusarium wilt races 4 and 5. Theo Appl Genet. 101(7): 1155- 1163.
• Young, N. D. 1996. QTL mapping and quantitative disease resistance in plants. Annu. Rev. Phytopathol. 34: 479-501