G-proteins play important roles in a number of cellular and developmental processes in plants. In this study, we have cloned a 317-bp fragment encoding a part of barley G protein -subunit into -genome of barley stripe mosaic virus (BSMV). Barley seedlings were inoculated with this construct denoted as pSL038-1/G at 3-leaf stage, and assayed for their growth, leaf area and membrane ion leakage. Silencing was achieved after 2 weeks of inoculation and confirmed by the decrease in G subunit mRNAs in barley leaves. There was a significant decrease in plant growth in terms of plant height and leaf area following the viral infection. Leaf area in silenced plants were decreased by >10% compared to control plants (wild-type or inoculated by BSMV-PDS constructs). In conclusion, silencing resulted in etiolated phenotypes and growth retardation. Our optimized VIGS protocol in barley could be adopted to evaluate extensive physiological parameters and molecular changes in G silenced plants.
BSMV G-proteins Ion Leakage Phytoene desaturase (PDS) Virus induced gene silencing (VIGS)
Birincil Dil | İngilizce |
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Bölüm | Articles |
Yazarlar | |
Yayımlanma Tarihi | 26 Kasım 2015 |
Yayımlandığı Sayı | Yıl 2015 Cilt: 20 Sayı: 2 |
Turkish Journal of Field Crops is published by the Society of Field Crops Science and issued twice a year.
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Ege University, Faculty of Agriculture,Department of Field Crops
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