GENETIC VARIATION AMONG EGYPTIAN WHITE LUPIN (LUPINUS ALBUS L.) GENOTYPES

Year 2016, Volume: 21 Issue: 1, 148 - 155, 01.06.2016

Ehab El-harty Azzam Ashrıe Azzam Ashrıe Megahed Ammar Salem Alghamdı

https://doi.org/10.17557/tjfc.95532

Cited By: 3

Abstract

Field Evaluation of eighteen lupin genotypes was carried out during 2012/13 and 2013/14 at Giza Agriculture
Research Station, Egypt, to assess the genetic variation on phenological parameters. Simultaneously,
molecular diversity assessment was performed using 11 SRAP primer pair combinations. High significant
differences among genotypes were observed for field performance during the two seasons and their combined
data. The Australian genotype 75 B9.10 and landrace Fayed1 recorded the highest seed yield/plant while the
Egyptian landraces Sohag2, Fayed1 and the cultivar Giza1 were superiors in seed yield/hectare (2.8, 2.6 and
2.6t, respectively). Only the first two principle components explained 97 % of variability. Characters, number
of pods, seed yield/plant and seed yield/hectare were grouped on the positive PC1 axis of the biplot with
genotypes Sohag2, 75B9.10 and Fayed1. The molecular analysis revealed coherent results. The 11 SRAP
primers generated 3286 amplified fragments represents 337 genetic loci across the lupin genome. The average
detected loci per primer pair was 30.64 with 322 polymorphic loci across the studied genotypes (96.05%). The
polymorphism information content (PIC) values were generally high and ranged from 0.883 to 0.981. The high
PIC values highlight the power of SRAP markers in detecting the molecular diversity in lupin genotypes. The
genotypes tended to cluster based on their origin and genetic background.

Keywords

biplot, cluster analysis, lupin, marker, SRAP

References

• Alghamdi, S., S. Al-Faifi, H. Migdadi, M. Khan, E. EL-Harty and M. Ammar 2012. Molecular diversity assessment using sequence related amplified polymorphism (SRAP) markers in Vicia faba L. Int. J. Mol. Sci. 13 :16457–16471.
• Aneja, B., N.R. Yadav, V. Chawla and R.C. Yadav 2012. Sequence-related amplified polymorphism (SRAP) molecular marker system and its applications in crop improvement. Molecular Breeding 30 :1635–1648.
• Annicchiarico, P. 2008. Adaptation of cool-season grain legume species across climatically-contrasting environments of southern Europe. Agron. J. 100 :1647–1654.
• Annicchiarico, P., N. Harzic and A.M. Carroni 2010. Adaptation,diversity, and exploitation of global white lupin (Lupinus albus L.) landrace genetic resources. Field Crops Research, 119 :114-124.
• Arnoldi, A. 2005. The Healthy-Profood Project: optimized process for preparing healthy and added value food ingredients from lupin kernels, the European protein-rich legume. In: Final Conference of Healthy-Profood :1-8 Milan 9-18 November.
• Bhardwaj, H. L. and A.A. Hamama 2012. Yield and Nutritional Quality Traits of White Lupin Sprouts. Journal of Agricultural Science 4 :58-61.
• Budak, H., R.C. Shearman and D. Parmaksiz 2004. Comparative analysis of seeded and vegetative biotype buffalo grasses based o phylogenetic relationship using ISSRs, SSRs, RAPDs, and SRAPs. Theor. Appl. Genet. 109 :280-288.
• Christiansen, J.L., S. Raza, B. Jornsgard, S.A. Mahmoud and R. Ortiz 2000. Potential of landrace germplasm for genetic enhancement of white lupin in Egypt. Genetic Resources and Crop Evolution 47 :425–430.
• El-Sayad, Z. S. and E. El-Barougy 2002. Development of some white lupin mutant lines high yielding, early maturing and resistant to Fusarium wilt. Annals Agric. Sci. 47 : 641- 657.
• EL-Sherif, N.A., A.A. Mohamed, M.E. Saad, H. Barakat and S. Aly 2014. Genetic variation in Egyptian white lupin (Lupinus albus L.) genotypes based on combined data of ISSR and fluorescence-based AFLP markers. Egypt. J. Genet. Cytol. 43 :1-23.
• Esposito, M.A., E.A. Martin, V.P. Cravero and E. Cointry 2007. Characterization of pea accessions by SRAP’s markers. Sci. Hortic. 113 :329-335.
• FAOSTAT, 2014. http://faostat3.fao.org/home/E Faligowska, A. and J. Szukała 2015. The effect of various longterm tillage systems on yield and yield component of yellow and narrow-leaved lupin. Turk J Field Crops 20 (2) :188- 193.
• Ferrio, M., B. Pico and F. Nuez 2003. Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP markers. Theor. Appl. Genet. 107 :271-282.
• Fu, X., G. Ning, L. Gao and M. Bao 2008. Genetic diversity of Dianthus accessions as assessed using two molecular marker systems (SRAPs and ISSRs) and morphological traits. Sci. Hortic. 117 :263-270.
• Gilbert, G.A., J.D. Knight, C.P. Vance and D.L. Allan 1999. Acid phosphatase activity in phosphorus -deficient white lupin roots. Plant Cell and Environment 22 :801-810.
• Gladstones, J.S. 1998. Distribution, Origin, Taxonomy, History and Importance. In: Lupin as Crop Plants. eds. J.S.
• Gladstones, C.A. Atkins and J. Hamblin et al. :1-39. Biology, Production and Utilization. CAB International, Oxon, UK.
• Gonzalez-Andres, F., P. A. Casquero, C. San-Pedro and E. Hernandez-Sanchez 2007. Diversity in white lupin (Lupinus albus L.) landraces from northwest Iberian plateau. Genetic Resources and Crop Evolution 54 :27–44.
• Hefany, M. M. 2013. Use of genetic variability estimates and interrelationships of agronomic and biochemical characters for selection of lupin genotypes under different irrigation regimes. African Crop Science Journal 21 :97 – 108.
• Hill, G. D. 1977. The composition and nutritive value of lupin seed. Live-stock feeds & feeding 47: 511-529.
• Hondelmann, W. 1984. The lupin: Ancient and modern crop plant. Theor. Appl. Genet., 68 :1–9.
• Jaccard, P. 1908. Nouvelles recherches sur la distribution florale. Bull. Soc. Vaudoise Sci. Nat. 44 :223-270.
• Jansen, P. C. M. 2006. Lupinus albus (L.) Record from Protabase. In: PROTA (Plant Resources of Tropical Africa. eds. Brink, M. and G. Belay, Wageningen, Netherlands.
• Kurlovich, B.S. 2002. Lupins: Geography, Classification, Genetic Resources and Breeding. St. Petersburg, Publiching house " Intan".
• Lagunes-Espinoza, L.C., C. Huyghe and J. Papineau 2000. Genetic variation for pod wall proportion in Lupinus albus L. Plant Breed. 119 :421-425.
• Liu, J., Z.R. Wang, C. Li, Y.B. Bian and Y. Xiao 2015. Genetic diversity of Pleurotus pulmonarius revealed by RAPD, ISSR, and SRAP fingerprinting. J. Basic. Microbiol. 55 :749-60.
• Lopez-Bellido, L., M. Fuentes and J.E. Castillo 2000. Growth and yield of white lupin under Mediterranean conditions: Effect of plant density. Agronomy Journal 92 :200-205.
• Mut, H., A. Gulumser, I. Ayan, Z. Acar, U. Basaran and O. Onal-Asci 2012. Effects of cultivar, inoculation, and sowing date in seed yield and yield components of lupin. Journal of Plant Nutrition, 35 (9) :1290-1302.
• Mulayim, M., A. Tamkoc and M. Babaoglu 2002. Sweet white lupins versus local bitter genotype: agronomic characteristics as affected by different planting densities in the Goller region of Turkey. European Journal of Agronomy 17 :181– 189.
• Noffsinger, S.L., C. Huyghe and E. van Santen 2000. Analysis of grain-yield components and inflorescence levels in wintertype white lupin. Agron. J. 92 :1195–1202.
• Omer, M. A. M., E. A. Mohamed, I. A. M. Ahmed, A. A. Yagoub and E. E. Babiker 2016. Effect of different processing methods on anti-nutrients content and protein quality of improved lupin (Lupinus albus L.) cultivar seeds. Turkish Journal of Agriculture - Food Science and Technology, 4 (1): 9-16.
• Raman, R., D.J. Luckett and H. Raman 2008. Estimation of genetic diversity in albus lupin (Lupinus albus L.) using DART and genic markers. In :Lupins for Health and Wealth Proceedings of the 12th International Lupin Conference. eds. J.A. Palta and J.B. Berger, Fremantle, Western Australia. International Lupin Association, Canterbury, New Zealand. 14-18 Sept.
• Raza, S. and B. Jornsgard 2002. Screening of white lupin accessions for morphological and yield traits. African Crop Science Journal 13 :135–141.
• Robarts, D. W. H. and A.D. Wolfe 2014. Sequence-related amplified polymorphism (SRAP) markers: A potential resource for studies in plant molecular biology. Appl Plant Sci. 2 : http://dx.doi.org/10.3732/apps.1400017.
• Rocio, M., E. Esteban, P. Zornoza and M.J. Sierra 2013. Could an abandoned mercury mine area be cropped? Environmental Research 125 :150-159.
• Rohlf, F. 2005. Ntsys-pc, Numerical taxonomy and multivariate analysis system, version 2.2. Exeter Publishing Ltd., New York.
• Rubio, J., J.I. Cubero, L.M. Martyn, M.J. Suso and F. Flores 2004. Biplot analysis of trait relations of white lupin in Spain. Euphytica 135: 217-224.
• Sabaghnia, N. 2015. Investigation of some morphological traits in studied lentil (Lens culinaris Medik.) genotypes grown with foliar application of nanosized ferric oxide. Annales UMCS, Biologia. 69 :29–38.
• Sipsas, S. 2008. Lupin products - Concepts and reality. In Lupins for Health and Wealth. In :Proceedings of the 12th International Lupin Conference : 506-513 eds. J.A. Palta and J.B. Berger, Freemantle, Australia 14-18 September.
• Steel, R.G.D. and J.H. Torrie 1980. Principles and Procedures of Statistics: A Biometrical Approach. 2 ed. New York: McGraw-Hill Publ. Company.
• Tabrett S, D. Blyth, N. Bourne and B. Glencross 2012. Digestibility of Lupinus albus lupin mean in barramundi (Lates calcarifer). Aquaculture 364 :1-5.
• Wang, T., J. Xu, X.X. Zhang and L.J. Zhao 2012. Genetic relationship of 43 cultivars of Viola tricolor and Viola cornuta using SRAP marker. Scientia Agricultura Sinica 45 :496–502.
• Wilkins, R. J. and R. Jones 2000. Alternative home-grown protein sources for ruminants in the United Kingdom. Animal Feed Science and Technology 85 :23–32.
• Yorgancilar, M., M. Babaoglu, E.E. Hakki and E. Atalay 2009. Determination of the relationship among Old World Lupin (Lupinus sp.) species using RAPD and ISSR markers. African Journal of Biotechnology 8 :3524-3530.
• Zhukovsky, P.M. 1929. A contribution to the knowledge of genus Lupinus Tourn. Bull. Apll. Bot. Gen. Pl.-Breed. 3:16- 294.