PHYSIOLOGICAL RESPONSES TO HIGH EXOGENOUS SUCROSE IN TUBER INDUCTION OF POTATO IN VITRO

Year 2016, Volume: 21 Issue: 2, 254 - 260, 15.12.2016

Xueqin He Meilian Meng

https://doi.org/10.17557/tjfc.55137

Cited By: 1

Abstract

Microtubers are valuable tools to be used in the researches of potato. Among the factors sucrose is the most
critical stimulus for potato microtuber production in vitro. The aim of our study was to define whether only
sucrose was enough to induce the tuber and how it impacted on physiological responses in potato in vitro.
Among sucrose concentrations chosen 80 and 100g/l sucrose could induce maximum tubers in three cultivars
of Solanum tuberosum L., Atlantic, Desiree and 851 in vitro at 22 oC under 16-h photoperiod. Meanwhile tuber
induction percentage showed no difference in MS with 80 and 100g/l sucrose among 0, 16 and 24-h
photoperiod and cuttings with no nodes induced no tubers in cv. Atlantic. Therefore, 80 or 100g/l of exogenous
sucrose concentrations and cuttings with node were essential to in vitro tuber induction in potato. The
endogenous sucrose content and acid invertase in induced cuttings were higher than corresponding tubers and
dramatically increased with the increase of sucrose concentration in MS. Starch content was completely
opposite to endogenous sucrose content and decreased with the increase of sucrose concentration in MS.
Sucrose synthase (Susy) and sucrose phosphate synthase (SPS) accumulated only in tubers, but no significant
differences of Susy and SPS were between 80g/l and 100g/l sucrose. In conclusion, high levels of exogenous
sucrose resulted in a dramatic high gradient of endogenous sucrose content between inducing cuttings and the
corresponding tubers, which was beneficial to trigger differential activity expression of sugar metabolizing
enzymes and to accumulate starch in tubers in vitro. 

Keywords

Microtuber, potato, sucrose, sugar metabolic enzymes

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