INTEGRATION OF NOVEL SSR MARKERS INTO THE LENTIL (Lens culinaris Medik.) GENOME

Year 2024, Volume: 29 Issue: 1, 40 - 45, 21.06.2024

Brian Wakimwayi Koboyi Melike Bakır

https://doi.org/10.17557/tjfc.1415823

Abstract

The development of Simple Sequence Repeat markers (SSRs) for lentil has played a pivotal role in enhancing the comprehension of the lentil genome through genetic mapping. The study aimed to determine the relative positions of newly developed microsatellites to the lentil genome using an F7-derived Recombinant Inbred Lines population (RIL) of 71 individuals developed from a cross between Eston and PI320937. Molecular analysis was performed with 100 newly developed lentil SSR markers and a linkage map was constructed using MapMaker/EXP 3.0b and MapChart 2.2 software. Among the 100 SSR markers, 12 markers exhibited polymorphism, 54 markers were identified as monomorphic, and 34 markers remained unamplified. While 10 out of the 12 polymorphic markers successfully integrated into two linkage groups, covering a cumulative length of 19.2cM, two markers remained unlinked. Linkage group-1, comprised of 8 markers, spanned 4.8cM, and linkage group-2 extended over a length of 14.4cM with two markers. Despite only partially representing 2 out of the 7 chromosomes in the lentil genome, this map holds promise for future mapping studies. Through the addition of markers, it could facilitate marker-assisted selection and the identification of QTLs associated with specific agronomic traits.

Keywords

Linkage map, Microsatellites, Polymorphism, Recombinant Inbred Lines, Segregation Distortion

Ethical Statement

The authors declare no conflict of interest.

Project Number

FCD-2021-10106

Thanks

The authors gratefully acknowledge the financial support from the Erciyes University Scientific Research Unit (Project No: FCD-2021-10106). We are also thankful to Erciyes University Faculty of Agriculture and the Genome and Stem Cell Centre (Genkök) for availing laboratory equipment to enable the smooth implementation of this research.

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